Basic Information
ID: ALA3705746
Type: Binding
Description: In Vitro Inhibition Assay: An in vitro assay showed inhibition of CXCR2-mediated intracellular calcium release. Briefly, human neutrophils were suspended in HBSS- (without Ca2+ and Mg2+) containing 10 mM HEPES and FLIPR Calcium 3 dye (3.1x107 cells in total volume 1.7 mL). Cells were aliquoted (200 uL of the cell suspension per tube, 8 tubes total) and 2 uL of the designated compound (with appropriate dilutions) were added to each of 6 tubes. As controls, 2 uL of DMSO (1% final concentration) were added to 2 other tubes. Cells were incubated for 30 min at 37 C. After dye loading, tubes were centrifuged at 6,000 rpm for 1 min, supernatant was removed and the cell pellet was re-suspended in 200 uL of HBSS+ (with Ca2+ and Mg2+) containing 10 mM HEPES. The test compound or DMSO (control) was added again at the same concentrations that were used during cell loading. The cell suspension was aliquoted into a 96-well Reading Plate (Corning) in a volume of 90 uL (105 cells/well).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705746
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 382.23 | ||
| 330.35 | 2.28 | 2.28 |
| 402.47 | 4.00 | 4.00 |
| 307.31 | 2.80 | 2.80 |
| 398.40 | 4.79 | 4.79 |
| 346.43 | 4.13 | 4.13 |
| 435.55 | 6.20 | 6.20 |
| 339.40 | 4.16 | 4.16 |
| 479.58 | 5.61 | 5.61 |
| 346.43 | 3.99 | 3.99 |
| 420.47 | 5.12 | 5.12 |
| 396.37 | 5.38 | 5.38 |
| 332.40 | 3.74 | 3.74 |
| 370.45 | 4.15 | 4.15 |
| 373.37 | 4.27 | 4.27 |
| 343.38 | 4.06 | 4.06 |
| 419.48 | 5.73 | 5.73 |
| 419.48 | 5.73 | 5.73 |
| 503.55 | 7.22 | 7.22 |
| 435.55 | 6.20 | 6.20 |
| 338.41 | 4.77 | 4.77 |
| 383.40 | 4.67 | 4.67 |
| 422.40 | 5.66 | 5.66 |
| 422.40 | 5.66 | 5.66 |
| 420.53 | 6.49 | 6.49 |
| 405.46 | 4.35 | 4.35 |
| 420.47 | 5.12 | 5.12 |
| 404.47 | 4.95 | 4.95 |
| 435.55 | 6.20 | 6.20 |
| 403.48 | 5.56 | 5.56 |
| 403.48 | 5.56 | 5.56 |
| 370.42 | 5.21 | 5.21 |
| 407.30 | 6.07 | 6.07 |
| 354.41 | 4.47 | 4.47 |
| 396.44 | 4.55 | 4.55 |
| 396.44 | 4.55 | 4.55 |
| 382.42 | 4.46 | 4.46 |
| 396.44 | 4.55 | 4.55 |
| 382.42 | 4.46 | 4.46 |
| 406.45 | 3.95 | 3.95 |
| 464.37 | ||
| 448.23 | ||
| 408.24 | ||
| 432.27 | ||
| 365.22 | ||
| 380.23 | ||
| 383.21 | ||
| 473.34 | ||
| 473.34 | ||
| 479.39 | ||
| 440.26 | ||
| 466.22 | ||
| 412.25 | ||
| 442.28 | ||
| 348.21 | ||
| 382.23 | ||
| 400.22 | ||
| 426.23 | ||
| 396.25 | ||
| 382.23 | ||
| 396.44 | 4.69 | 4.69 |
| 438.40 | 5.37 | 5.37 |
| 480.44 | 5.59 | 5.59 |
| 478.40 | ||
| 516.45 | ||
| 482.36 | ||
| 548.37 | ||
| 464.37 | ||
| 420.53 | 6.49 | 6.49 |
| 399.40 | 4.38 | 4.38 |
| 419.49 | 4.65 | 4.65 |
| 410.47 | 4.93 | 4.93 |
| 396.44 | 4.41 | 4.41 |
| 426.47 | 4.42 | 4.42 |
| 389.46 | 5.31 | 5.31 |
| 389.46 | 5.31 | 5.31 |
| 403.48 | 5.62 | 5.62 |
| 491.90 | 6.99 | 6.99 |
| 422.45 | 2.66 | 2.66 |
| 480.48 | 4.16 | 4.16 |
| 494.50 | 4.55 | 4.55 |
| 522.56 | 5.33 | 5.33 |
| 392.46 | 5.92 | 5.92 |
| 396.47 | 4.77 | 4.77 |
| 430.92 | 5.42 | 5.42 |
| 396.47 | 4.77 | 4.77 |
| 380.40 | 4.30 | 4.30 |
| 380.40 | 4.30 | 4.30 |
| 392.46 | 4.65 | 4.65 |
| 395.48 | 5.38 | 5.38 |
| 428.94 | 6.63 | 6.63 |
| 368.43 | 4.64 | 4.64 |
| 468.55 | 5.72 | 5.72 |
| 517.61 | 5.96 | 5.96 |
| 346.38 | 3.13 | 3.13 |
| 380.44 | 4.76 | 4.76 |
| 434.41 | 5.47 | 5.47 |
| 394.47 | 5.06 | 5.06 |
| 445.31 | 5.21 | 5.21 |
| 445.31 | 5.21 | 5.21 |
| 400.86 | 5.10 | 5.10 |
| 384.41 | 4.59 | 4.59 |
| 414.89 | 5.41 | 5.41 |
| 435.31 | 5.75 | 5.75 |
| 445.86 | 5.01 | 5.01 |
| 402.40 | 4.73 | 4.73 |
| 494.63 | 6.75 | 6.75 |
| 436.55 | 6.18 | 6.18 |
| 448.54 | 6.18 | 6.18 |
| 451.52 | 4.28 | 4.28 |
| 396.44 | 4.46 | 4.46 |
| 411.41 | 4.36 | 4.36 |
| 391.43 | 4.32 | 4.32 |
| 391.43 | 4.32 | 4.32 |
| 434.46 | 3.63 | 3.63 |
| 444.51 | 3.85 | 3.85 |
| 450.41 | 5.35 | 5.35 |
| 432.42 | 5.05 | 5.05 |
| 434.41 | 5.47 | 5.47 |
| 434.41 | 5.47 | 5.47 |
| 502.41 | 6.49 | 6.49 |
| 476.62 | 6.80 | 6.80 |
| 504.67 | 7.36 | 7.36 |
| 367.40 | 3.84 | 3.84 |
| 367.40 | 3.84 | 3.84 |
| 367.40 | 3.84 | 3.84 |
| 356.38 | 4.04 | 4.04 |
| 406.44 | 5.19 | 5.19 |
| 406.44 | 5.19 | 5.19 |
| 485.33 | 5.96 | 5.96 |
| 406.44 | 5.19 | 5.19 |
| 372.45 | 4.51 | 4.51 |
| 372.45 | 4.51 | 4.51 |
| 448.54 | 6.18 | 6.18 |
| 449.53 | 5.57 | 5.57 |
| 422.51 | 5.66 | 5.66 |
| 422.51 | 5.66 | 5.66 |
| 501.40 | 6.43 | 6.43 |
| 422.51 | 5.66 | 5.66 |
| 436.53 | 5.97 | 5.97 |
| 450.56 | 6.28 | 6.28 |
| 447.49 | 5.41 | 5.41 |
| 470.98 | 6.62 | 6.62 |
| 433.46 | 5.10 | 5.10 |
| 467.91 | 5.76 | 5.76 |
| 502.35 | 6.41 | 6.41 |
| 502.35 | 6.41 | 6.41 |
| 415.40 | 3.22 | 3.22 |
| 401.38 | 3.13 | 3.13 |
| 410.43 | 4.18 | 4.18 |
| 424.45 | 4.22 | 4.22 |
| 424.45 | 4.22 | 4.22 |
| 438.48 | 4.61 | 4.61 |
| 438.48 | 4.61 | 4.61 |
| 373.43 | 3.90 | 3.90 |
| 423.49 | 5.06 | 5.06 |
| 463.56 | 5.88 | 5.88 |
| 498.00 | 6.53 | 6.53 |
| 531.56 | 6.90 | 6.90 |
| 465.54 | 4.67 | 4.67 |
| 446.51 | 4.94 | 4.94 |
| 420.47 | 4.33 | 4.33 |
| 487.35 | 5.15 | 5.15 |
| 456.54 | 5.97 | 5.97 |
| 396.25 | ||
| 398.22 | ||
| 426.23 | ||
| 384.20 | ||
| 367.14 | ||
| 323.33 | 3.45 | 3.45 |
| 417.66 | ||
| 355.39 | 3.18 | 3.18 |
| 371.39 | 2.84 | 2.84 |
| 371.39 | 2.88 | 2.88 |
| 387.39 | 2.55 | 2.55 |
| 455.39 | 3.78 | 3.78 |
| 471.39 | 3.45 | 3.45 |