Basic Information
ID: ALA3705762
Type: Binding
Description: IMAP Assay : Condition 1: Activated ERK2 activity was also determined in the IMAP assay format using the procedure outlined above. 1 μl of 25× compound was added to 140 of kinase buffer containing 0.25 ng fully phosphorylated, active Mouse ERK2 protein. Following a 30 minute incubation, the reactions were initiated by addition of 10 μl of kinase buffer containing 1 μM ERK2 IMAP substrate peptide (0.9 μM unlabeled IPTTPITTTYFFFK-CONH2 and 100 nM IPTTPITTTYFFFK(5-carboxyfluorescein)-CONH2) and 30 μM ATP. Reactions proceeded for 30 minutes before termination by addition of 60 μl IMAP detection beads in binding buffer. Plates were read as above after 30 minute binding equilibration. Active compounds were reconfirmed in an independent assay.
Organism: Mus musculus
Activity Charts
Bioactivity
Activity Types for Assay ALA3705762
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 463.94 | 4.81 | 4.81 |
| 486.59 | 5.55 | 5.55 |
| 487.58 | 4.95 | 4.95 |
| 470.55 | 4.70 | 4.70 |
| 397.48 | 3.96 | 3.96 |
| 411.51 | 4.02 | 4.02 |
| 429.50 | 4.16 | 4.16 |
| 411.51 | 4.02 | 4.02 |
| 429.50 | 4.16 | 4.16 |
| 411.51 | 3.84 | 3.84 |
| 453.55 | 3.95 | 3.95 |
| 520.56 | 5.58 | 5.58 |
| 477.57 | 4.43 | 4.43 |
| 453.55 | 3.95 | 3.95 |
| 477.57 | 4.43 | 4.43 |
| 459.58 | 4.02 | 4.02 |
| 495.63 | 4.63 | 4.63 |
| 488.54 | 4.84 | 4.84 |
| 492.58 | 5.31 | 5.31 |
| 600.70 | 3.83 | 3.83 |
| 429.50 | 4.16 | 4.16 |
| 411.51 | 4.02 | 4.02 |
| 487.58 | 4.95 | 4.95 |
| 452.56 | 4.56 | 4.56 |
| 470.55 | 4.70 | 4.70 |
| 425.54 | 4.58 | 4.58 |
| 438.53 | 4.04 | 4.04 |
| 452.56 | 4.38 | 4.38 |
| 440.50 | 3.83 | 3.83 |
| 452.52 | 3.28 | 3.28 |
| 454.57 | 4.89 | 4.89 |
| 482.63 | 5.62 | 5.62 |
| 468.60 | 5.23 | 5.23 |
| 455.56 | 4.29 | 4.29 |
| 469.59 | 4.63 | 4.63 |
| 483.62 | 5.02 | 5.02 |
| 538.65 | 5.69 | 5.69 |
| 436.52 | 3.98 | 3.98 |
| 455.56 | 4.29 | 4.29 |
| 459.53 | 4.17 | 4.17 |
| 469.56 | 5.04 | 5.04 |
| 457.55 | 4.72 | 4.72 |
| 443.53 | 4.47 | 4.47 |
| 497.50 | 5.18 | 5.18 |
| 500.57 | 4.71 | 4.71 |
| 443.53 | 4.40 | 4.40 |
| 505.62 | 5.05 | 5.05 |
| 441.54 | 4.03 | 4.03 |
| 473.55 | 4.48 | 4.48 |
| 461.52 | 4.61 | 4.61 |
| 501.56 | 4.43 | 4.43 |
| 531.59 | 4.44 | 4.44 |
| 519.55 | 4.57 | 4.57 |
| 513.60 | 4.30 | 4.30 |
| 483.62 | 5.12 | 5.12 |
| 514.63 | 5.99 | 5.99 |
| 498.56 | 5.52 | 5.52 |
| 498.56 | 4.65 | 4.65 |
| 565.67 | 4.02 | 4.02 |
| 515.59 | 4.73 | 4.73 |
| 500.57 | 4.97 | 4.97 |
| 500.57 | 4.97 | 4.97 |
| 483.55 | 4.64 | 4.64 |
| 483.55 | 4.64 | 4.64 |
| 536.63 | 4.18 | 4.18 |
| 531.59 | 4.28 | 4.28 |
| 481.63 | 5.54 | 5.54 |
| 459.98 | 4.98 | 4.98 |
| 506.61 | 4.94 | 4.94 |
| 453.59 | 4.94 | 4.94 |
| 478.60 | 4.82 | 4.82 |
| 478.60 | 4.82 | 4.82 |
| 476.56 | 3.99 | 3.99 |
| 498.56 | 4.42 | 4.42 |
| 513.57 | 5.22 | 5.22 |
| 513.57 | 5.22 | 5.22 |
| 465.56 | 5.07 | 5.07 |
| 512.42 | 5.77 | 5.77 |
| 481.63 | 5.54 | 5.54 |
| 427.51 | 3.12 | 3.12 |
| 456.55 | 3.73 | 3.73 |
| 451.57 | 4.82 | 4.82 |
| 487.58 | 4.72 | 4.72 |
| 512.59 | 4.67 | 4.67 |
| 512.59 | 4.67 | 4.67 |
| 526.62 | 4.90 | 4.90 |
| 526.62 | 4.90 | 4.90 |
| 503.58 | 4.10 | 4.10 |
| 529.64 | 5.69 | 5.69 |
| 487.54 | 4.00 | 4.00 |
| 512.55 | 3.95 | 3.95 |
| 531.59 | 3.87 | 3.87 |
| 444.51 | 3.86 | 3.86 |
| 446.58 | 4.09 | 4.09 |
| 461.59 | 4.40 | 4.40 |
| 542.57 | 3.73 | 3.73 |
| 439.56 | 4.89 | 4.89 |
| 427.51 | 3.12 | 3.12 |
| 528.59 | 3.64 | 3.64 |
| 457.55 | 4.51 | 4.51 |
| 507.56 | 5.39 | 5.39 |
| 471.54 | 4.15 | 4.15 |
| 524.60 | 4.66 | 4.66 |
| 512.56 | 4.79 | 4.79 |
| 454.57 | 4.89 | 4.89 |
| 459.53 | 3.46 | 3.46 |
| 415.47 | 4.16 | 4.16 |
| 473.55 | 4.56 | 4.56 |
| 461.52 | 4.69 | 4.69 |
| 443.53 | 4.55 | 4.55 |
| 426.52 | 3.80 | 3.80 |
| 439.56 | 4.45 | 4.45 |
| 481.93 | 4.95 | 4.95 |
| 425.54 | 4.41 | 4.41 |
| 491.50 | 4.40 | 4.40 |
| 495.51 | 4.41 | 4.41 |
| 483.47 | 4.54 | 4.54 |
| 321.38 | 2.11 | 2.11 |
| 453.59 | 4.84 | 4.84 |
| 473.55 | 4.00 | 4.00 |
| 566.56 | 5.21 | 5.21 |
| 566.56 | 5.21 | 5.21 |
| 628.83 | ||
| 514.56 | 3.39 | 3.39 |
| 516.55 | 4.37 | 4.37 |
| 379.42 | 1.65 | 1.65 |
| 457.55 | 4.59 | 4.59 |
| 469.55 | 3.56 | 3.56 |
| 499.55 | 3.81 | 3.81 |
| 513.58 | 4.12 | 4.12 |
| 499.55 | 3.81 | 3.81 |
| 487.58 | 4.86 | 4.86 |
| 487.58 | 4.86 | 4.86 |
| 493.53 | 5.06 | 5.06 |
| 475.54 | 4.92 | 4.92 |
| 489.62 | 5.61 | 5.61 |
| 457.55 | 4.59 | 4.59 |
| 515.61 | 5.38 | 5.38 |
| 527.60 | 4.38 | 4.38 |
| 414.51 | 3.48 | 3.48 |
| 538.63 | 5.20 | 5.20 |
| 403.45 | 2.40 | 2.40 |
| 465.48 | 4.40 | 4.40 |
| 447.49 | 4.26 | 4.26 |
| 457.55 | 4.59 | 4.59 |
| 469.55 | 3.56 | 3.56 |
| 453.55 | 4.09 | 4.09 |
| 516.60 | 4.78 | 4.78 |
| 500.53 | 4.31 | 4.31 |
| 567.55 | 4.83 | 4.83 |
| 515.61 | 5.38 | 5.38 |
| 512.55 | 3.95 | 3.95 |
| 462.56 | 4.27 | 4.27 |
| 482.59 | 3.48 | 3.48 |
| 469.59 | 4.46 | 4.46 |
| 544.66 | 4.70 | 4.70 |
| 482.59 | 3.52 | 3.52 |
| 552.72 | 5.18 | 5.18 |
| 465.60 | 4.94 | 4.94 |
| 454.53 | 2.79 | 2.79 |
| 454.53 | 2.79 | 2.79 |
| 543.67 | 5.94 | 5.94 |
| 534.54 | 4.66 | 4.66 |
| 522.51 | 4.80 | 4.80 |
| 498.54 | 4.55 | 4.55 |
| 510.57 | 4.42 | 4.42 |
| 468.56 | 3.13 | 3.13 |
| 477.52 | 4.27 | 4.27 |
| 475.54 | 4.88 | 4.88 |
| 517.56 | 3.71 | 3.71 |
| 529.64 | 5.63 | 5.63 |
| 568.55 | 5.58 | 5.58 |
| 568.55 | 5.58 | 5.58 |
| 489.55 | 3.53 | 3.53 |
| 447.49 | 4.30 | 4.30 |
| 475.54 | 4.73 | 4.73 |
| 503.58 | 4.18 | 4.18 |
| 459.53 | 4.17 | 4.17 |
| 530.63 | 5.02 | 5.02 |
| 579.60 | 6.58 | 6.58 |
| 513.58 | 4.06 | 4.06 |
| 514.56 | 4.55 | 4.55 |
| 529.64 | 5.63 | 5.63 |
| 416.49 | 2.15 | 2.15 |
| 503.58 | 4.18 | 4.18 |
| 516.58 | 3.28 | 3.28 |
| 483.57 | 3.81 | 3.81 |
| 477.52 | 3.58 | 3.58 |
| 459.53 | 3.44 | 3.44 |
| 459.53 | 3.44 | 3.44 |
| 469.56 | 5.00 | 5.00 |
| 499.59 | 5.01 | 5.01 |
| 530.60 | 3.62 | 3.62 |
| 502.55 | 3.02 | 3.02 |
| 558.66 | 4.40 | 4.40 |
| 570.67 | 4.55 | 4.55 |
| 556.64 | 4.16 | 4.16 |
| 606.65 | 4.79 | 4.79 |
| 544.63 | 3.93 | 3.93 |
| 572.69 | 4.71 | 4.71 |
| 592.62 | 4.32 | 4.32 |
| 556.64 | 4.08 | 4.08 |
| 586.67 | 3.70 | 3.70 |
| 650.75 | 5.18 | 5.18 |
| 574.66 | 3.61 | 3.61 |
| 558.66 | 4.37 | 4.37 |
| 584.70 | 4.90 | 4.90 |
| 560.63 | 2.95 | 2.95 |
| 600.74 | 5.49 | 5.49 |
| 628.79 | 6.27 | 6.27 |
| 558.66 | 4.32 | 4.32 |
| 572.69 | 4.71 | 4.71 |
| 560.65 | 4.84 | 4.84 |
| 532.60 | 4.06 | 4.06 |
| 580.59 | 4.45 | 4.45 |
| 514.61 | 3.92 | 3.92 |
| 542.66 | 4.70 | 4.70 |
| 562.60 | 4.31 | 4.31 |
| 502.64 | 3.85 | 3.85 |
| 550.64 | 4.24 | 4.24 |
| 530.70 | 4.63 | 4.63 |
| 583.67 | 4.12 | 4.12 |
| 611.72 | 4.90 | 4.90 |
| 631.66 | 4.50 | 4.50 |
| 600.72 | 5.15 | 5.15 |
| 638.73 | 5.12 | 5.12 |
| 510.64 | 3.83 | 3.83 |
| 538.70 | 4.61 | 4.61 |
| 558.63 | 4.22 | 4.22 |
| 565.70 | 4.83 | 4.83 |
| 628.77 | 5.93 | 5.93 |
| 648.71 | 5.54 | 5.54 |
| 572.67 | 4.54 | 4.54 |
| 516.58 | 3.33 | 3.33 |
| 531.59 | 2.83 | 2.83 |
| 559.65 | 3.44 | 3.44 |
| 685.80 | 4.92 | 4.92 |
| 593.10 | 4.59 | 4.59 |
| 619.14 | 5.07 | 5.07 |
| 560.63 | 3.86 | 3.86 |
| 545.62 | 3.09 | 3.09 |
| 585.68 | 3.28 | 3.28 |
| 656.76 | 3.72 | 3.72 |
| 530.60 | 3.59 | 3.59 |
| 604.71 | 2.97 | 2.97 |
| 531.59 | 3.02 | 3.02 |
| 562.62 | 3.93 | 3.93 |
| 598.60 | 4.52 | 4.52 |
| 559.65 | 3.80 | 3.80 |
| 579.58 | 3.41 | 3.41 |
| 572.64 | 4.01 | 4.01 |
| 586.67 | 4.40 | 4.40 |
| 599.71 | 3.62 | 3.62 |
| 627.72 | 3.53 | 3.53 |
| 643.72 | 3.75 | 3.75 |
| 657.75 | 4.14 | 4.14 |
| 659.76 | 4.48 | 4.48 |
| 673.79 | 4.88 | 4.88 |
| 683.79 | 4.68 | 4.68 |
| 702.90 | ||
| 588.68 | 4.69 | 4.69 |
| 701.84 | 5.61 | 5.61 |
| 697.81 | 5.07 | 5.07 |
| 671.77 | 4.53 | 4.53 |
| 713.81 | 4.30 | 4.30 |
| 425.54 | 3.92 | 3.92 |
| 514.58 | 4.69 | 4.69 |
| 528.61 | 5.08 | 5.08 |
| 475.54 | 4.50 | 4.50 |
| 515.57 | 4.15 | 4.15 |
| 515.57 | 4.15 | 4.15 |
| 500.55 | 4.45 | 4.45 |
| 500.55 | 4.45 | 4.45 |
| 501.54 | 3.84 | 3.84 |
| 502.53 | 4.34 | 4.34 |
| 502.53 | 4.34 | 4.34 |
| 517.61 | 5.41 | 5.41 |
| 517.61 | 5.41 | 5.41 |
| 518.59 | 4.81 | 4.81 |
| 464.57 | 4.21 | 4.21 |