Assay Report Card

Basic Information

ID: ALA3705773

Type: Binding

Description: Calcium Flux Assay: Experiments were conducted using the FLIPRTETRA®. On the day prior to the experiment, recombinant HEK293 cells that stably express human and mouse TRPV3 were removed from tissue culture flasks and plated in growth medium at 20,000 cells/well into black-walled clear-bottom 384-well Biocoat poly-D-lysine assay plates (BD Biosciences, Bedford, Mass.) using a Multidrop® dispenser (ThermoScientific, Waltham, Mass.). On the day of the experiment, growth medium was removed, and the no-wash FLIPR® Calcium-4 dye (λEX=470-495 nm, λEM=515-575 nm; Molecular Devices, Sunnyvale, Calif.) was added to each well using the Multidrop® dispenser. Cells were incubated for 90-120 minutes in the dark. Compounds were dissolved in DMSO to prepare a 10 mM stock solution. The intensity of the fluorescence was captured and digitally transferred to an interfaced PC.

Organism: Homo sapiens

Cell type: HEK293

Activity Charts

Bioactivity

Activity Types for Assay ALA3705773

IC50

Total(291)

Compound Summary

Parent Molecular Weight	ALogP	Polar Surface Area
350.38	4.16	4.16
352.40	4.26	4.26
338.37	3.87	3.87
350.38	4.16	4.16
350.38	4.16	4.16
350.38	4.16	4.16
350.38	4.16	4.16
338.37	3.88	3.88
277.41	2.18	2.18
300.40	2.77	2.77
306.36	3.13	3.13
306.36	3.13	3.13
322.81	3.65	3.65
356.36	4.01	4.01
356.36	4.01	4.01
372.36	3.89	3.89
372.36	3.89	3.89
357.26	4.30	4.30
357.26	4.30	4.30
424.36	5.03	5.03
364.44	3.92	3.92
346.45	3.78	3.78
364.44	3.92	3.92
306.36	3.13	3.13
357.35	3.41	3.41
356.36	4.01	4.01
357.35	3.41	3.41
289.35	2.39	2.39
339.36	3.27	3.27
305.81	2.90	2.90
357.35	3.41	3.41
307.34	2.53	2.53
348.39	3.34	3.34
323.80	3.04	3.04
395.50	2.24	2.24
330.40	3.20	3.20
387.50	3.48	3.48
322.81	3.65	3.65
331.44	3.06	3.06
373.47	2.83	2.83
399.51	3.62	3.62
413.54	3.87	3.87
390.81	4.67	4.67
435.57	3.17	3.17
357.47	3.59	3.59
409.53	2.55	2.55
423.55	3.02	3.02
427.56	4.40	4.40
386.39	3.94	3.94
437.54	2.02	2.02
374.46	2.23	2.23
415.55	4.25	4.25
347.43	3.18	3.18
435.54	4.27	4.27
434.46	3.60	3.60
421.54	2.78	2.78
437.58	3.41	3.41
417.55	3.18	3.18
341.43	3.49	3.49
341.43	3.49	3.49
363.40	3.11	3.11
363.40	3.11	3.11
349.37	2.72	2.72
339.36	3.27	3.27
343.45	2.89	2.89
339.41	3.54	3.54
357.35	3.41	3.41
358.34	3.00	3.00
355.34	3.16	3.16
385.36	3.08	3.08
342.34	3.77	3.77
343.32	3.16	3.16
354.35	3.91	3.91
368.37	4.30	4.30
382.40	4.69	4.69
328.31	3.38	3.38
356.36	4.16	4.16
396.43	4.94	4.94
370.39	4.40	4.40
342.34	3.77	3.77
357.35	3.55	3.55
397.42	4.33	4.33
371.38	3.80	3.80
343.32	3.16	3.16
339.36	3.27	3.27
331.38	2.56	2.56
347.41	2.99	2.99
347.41	2.99	2.99
363.86	3.50	3.50
397.42	3.87	3.87
361.44	3.03	3.03
361.44	3.03	3.03
361.44	3.03	3.03
377.89	3.54	3.54
411.44	3.91	3.91
411.44	3.91	3.91
384.42	4.79	4.79
385.41	4.19	4.19
370.39	4.40	4.40
371.38	3.80	3.80
357.35	3.41	3.41
373.81	3.92	3.92
353.39	3.58	3.58
369.39	3.28	3.28
358.34	2.80	2.80
388.37	2.73	2.73
357.35	3.55	3.55
369.36	3.51	3.51
370.35	2.90	2.90
407.36	4.29	4.29
370.35	2.90	2.90
372.32	1.63	1.63
359.32	2.13	2.13
369.36	3.69	3.69
369.36	3.69	3.69
314.40	3.39	3.39
332.39	3.53	3.53
292.40	3.28	3.28
316.42	3.48	3.48
334.41	3.62	3.62
357.35	3.41	3.41
337.35	3.17	3.17
351.37	3.56	3.56
365.40	3.95	3.95
323.32	2.78	2.78
325.33	3.02	3.02
311.31	2.63	2.63
325.33	3.02	3.02
387.40	3.86	3.86
339.36	3.41	3.41
353.39	3.66	3.66
353.39	3.66	3.66
341.33	2.26	2.26
337.35	3.02	3.02
379.43	4.19	4.19
353.39	3.66	3.66
325.33	3.02	3.02
341.33	1.99	1.99
341.33	1.99	1.99
341.33	1.99	1.99
341.33	1.99	1.99
403.40	3.35	3.35
403.40	3.35	3.35
339.36	3.27	3.27
339.36	3.27	3.27
351.37	3.41	3.41
365.40	3.80	3.80
379.43	4.19	4.19
387.40	4.38	4.38
387.40	4.38	4.38
387.40	4.38	4.38
365.40	3.80	3.80
365.40	3.80	3.80
365.40	3.80	3.80
365.40	3.80	3.80
351.37	3.56	3.56
351.37	3.56	3.56
337.35	3.17	3.17
337.35	3.17	3.17
325.33	3.02	3.02
323.32	2.78	2.78
351.37	3.56	3.56
365.40	3.95	3.95
365.28	3.18	3.18
311.31	2.63	2.63
325.33	3.02	3.02
373.38	3.99	3.99
311.31	2.63	2.63
340.35	2.66	2.66
366.39	3.34	3.34
326.32	2.42	2.42
342.32	1.65	1.65
338.33	2.42	2.42
356.35	1.78	1.78
326.32	2.42	2.42
342.32	1.39	1.39
340.35	2.66	2.66
366.39	3.34	3.34
326.32	2.42	2.42
342.32	1.65	1.65
338.33	2.42	2.42
342.32	1.39	1.39
308.44	3.62	3.62
322.47	4.01	4.01
322.47	4.01	4.01
330.39	3.62	3.62
296.39	2.22	2.22
395.52	3.44	3.44
403.43	2.54	2.54
429.46	3.22	3.22
389.40	2.30	2.30
405.40	1.53	1.53
419.43	1.66	1.66
405.40	1.27	1.27
350.52	4.65	4.65
352.45	2.73	2.73
352.45	2.73	2.73
362.41	4.16	4.16
342.46	3.60	3.60
296.39	2.22	2.22
395.52	3.44	3.44
280.39	2.98	2.98
282.36	1.83	1.83
381.49	3.05	3.05
360.39	4.07	4.07
328.38	3.53	3.53
270.38	2.86	2.86
342.56
390.67
324.35	3.27	3.27
344.46	4.21	4.21
332.37	1.96	1.96
332.39	3.64	3.64
342.41	2.74	2.74
332.46	3.65	3.65
328.53
330.38	2.87	2.87
318.44	3.26	3.26
368.50	4.02	4.02
308.42	3.36	3.36
342.34	3.41	3.41
342.34	3.41	3.41
342.34	3.41	3.41
324.35	3.27	3.27
341.43	3.49	3.49
341.43	3.49	3.49
364.42	1.56	1.56
278.33	2.59	2.59
292.36	1.73	1.73
388.49	2.53	2.53
387.50	2.14	2.14
344.46	4.21	4.21
328.39	3.74	3.74
362.41	4.16	4.16
362.41	4.16	4.16
533.64	6.31	6.31
354.43	3.18	3.18
333.41	1.85	1.85
312.39	2.73	2.73
330.38	2.87	2.87
334.82	3.94	3.94
356.36	4.01	4.01
368.37	4.30	4.30
313.38	2.87	2.87
406.37	4.89	4.89
427.52	4.73	4.73
327.40	3.48	3.48
413.54	3.87	3.87
385.48	3.23	3.23
415.55	4.26	4.26
323.80	3.04	3.04
331.37	3.00	3.00
320.38	3.44	3.44
374.35	4.15	4.15
374.35	4.15	4.15
354.37	3.28	3.28
330.38	2.87	2.87
312.39	2.73	2.73
328.84	3.24	3.24
385.51	4.43	4.43
346.49	4.04	4.04
312.39	2.73	2.73
360.36	2.75	2.75
357.35	3.41	3.41
424.36	5.03	5.03
340.80	3.79	3.79
263.38	1.79	1.79
376.42	4.26	4.26
280.41	2.76	2.76
354.35	3.77	3.77
387.38	3.33	3.33
329.42	2.85	2.85
308.44	3.77	3.77
498.48	4.93	4.93
373.38	3.99	3.99
353.34	2.26	2.26
381.40	3.04	3.04
377.41	3.66	3.66
377.41	3.66	3.66
386.37	1.70	1.70
322.47	4.15	4.15
345.44	3.60	3.60
352.40	3.39	3.39
328.84	3.71	3.71
376.93	4.69	4.69
418.38	5.18	5.18
418.38	5.18	5.18
362.39	4.07	4.07
306.43	3.23	3.23