Basic Information
ID: ALA3705824
Type: Binding
Description: Radioligand Binding Assay: Human Embryonic Kidney 293 cells (HEK293) stably expressing rat orexin 1 receptor (Genebank accession number NM001525) or Chinese ovary cells (CHO) stably expressing human orexin 1 receptor (Genebank accession number NM001526) were grown to confluency in DMEM (Hyclone, cat #SH30022), 10% FBS, 1x Pen/Strep, 1x sodium pyruvate, 10 mM HEPES, 600 ug/mL G418 and DMEM/F12 (Gibco, Cat #11039), 10% FBS, 1x Pen/Strep, 600 ug/mL G418 media, respectively on 150 cm2 tissue culture plates, washed with 5 mM EDTA in PBS (HyClone Dulbecco's Phosphate Buffered Saline 1x with Calcium and Magnesium, Cat # SH30264.01, hereafter referred to simply as PBS) and scraped into 50 ml tubes. After centrifugation (2K xG, 5 min at 4 C.), the supernatant was aspirated and the pellets frozen and stored at -800 C. Cells were resuspended in PBS in the presence of 1 tablet of protease inhibitor cocktail (Roche, Cat. #11836145001) per 50 mL.
Organism: Homo sapiens
Cell type: ovary
Activity Charts
Bioactivity
Activity Types for Assay ALA3705824
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 404.45 | 3.75 | 3.75 |
| 401.47 | 3.31 | 3.31 |
| 390.45 | 2.44 | 2.44 |
| 408.44 | 2.58 | 2.58 |
| 407.45 | 3.18 | 3.18 |
| 408.50 | 5.02 | 5.02 |
| 458.44 | 3.46 | 3.46 |
| 457.46 | 4.06 | 4.06 |
| 457.46 | 4.06 | 4.06 |
| 457.46 | 4.06 | 4.06 |
| 403.49 | 3.35 | 3.35 |
| 403.49 | 3.35 | 3.35 |
| 403.49 | 3.35 | 3.35 |
| 418.50 | 3.05 | 3.05 |
| 508.50 | 4.61 | 4.61 |
| 393.42 | 2.87 | 2.87 |
| 377.42 | 3.84 | 3.84 |
| 376.43 | 4.45 | 4.45 |
| 390.46 | 4.75 | 4.75 |
| 392.43 | 3.48 | 3.48 |
| 392.43 | 4.55 | 4.55 |
| 420.48 | 4.84 | 4.84 |
| 441.50 | 4.86 | 4.86 |
| 411.41 | 3.01 | 3.01 |
| 422.44 | 3.89 | 3.89 |
| 422.44 | 3.89 | 3.89 |
| 418.47 | 4.06 | 4.06 |
| 426.48 | 3.28 | 3.28 |
| 444.47 | 3.42 | 3.42 |
| 440.51 | 3.59 | 3.59 |
| 444.47 | 3.42 | 3.42 |
| 451.53 | 4.47 | 4.47 |
| 418.50 | 3.05 | 3.05 |
| 418.50 | 4.13 | 4.13 |
| 431.54 | 4.72 | 4.72 |
| 381.48 | 3.56 | 3.56 |
| 433.49 | 3.76 | 3.76 |
| 433.49 | 3.76 | 3.76 |
| 429.52 | 3.93 | 3.93 |
| 419.55 | 4.89 | 4.89 |
| 469.34 | 3.20 | 3.20 |
| 404.47 | 2.75 | 2.75 |
| 390.45 | 2.44 | 2.44 |
| 404.47 | 2.75 | 2.75 |
| 408.43 | 3.65 | 3.65 |
| 419.46 | 3.45 | 3.45 |
| 423.45 | 3.35 | 3.35 |
| 421.48 | 3.49 | 3.49 |
| 410.49 | 3.60 | 3.60 |
| 422.46 | 3.96 | 3.96 |
| 407.45 | 3.18 | 3.18 |
| 390.45 | 2.44 | 2.44 |
| 403.48 | 4.10 | 4.10 |
| 388.47 | 3.55 | 3.55 |
| 403.48 | 4.10 | 4.10 |
| 389.46 | 3.71 | 3.71 |
| 402.49 | 4.71 | 4.71 |
| 405.52 | 4.59 | 4.59 |
| 390.44 | 3.51 | 3.51 |
| 404.47 | 3.82 | 3.82 |
| 403.49 | 3.35 | 3.35 |
| 392.50 | 3.46 | 3.46 |
| 423.45 | 2.88 | 2.88 |
| 410.42 | 3.82 | 3.82 |
| 407.45 | 3.18 | 3.18 |
| 424.44 | 2.28 | 2.28 |
| 411.41 | 3.01 | 3.01 |
| 411.41 | 3.01 | 3.01 |
| 411.41 | 3.01 | 3.01 |
| 427.87 | 3.53 | 3.53 |
| 407.45 | 3.18 | 3.18 |
| 418.47 | 4.06 | 4.06 |
| 422.44 | 3.89 | 3.89 |
| 407.45 | 3.18 | 3.18 |
| 423.45 | 2.37 | 2.37 |
| 390.44 | 3.51 | 3.51 |
| 390.45 | 2.44 | 2.44 |
| 393.42 | 2.87 | 2.87 |
| 390.45 | 2.44 | 2.44 |
| 459.43 | 2.85 | 2.85 |
| 459.43 | 2.85 | 2.85 |
| 459.43 | 3.93 | 3.93 |
| 462.41 | 3.29 | 3.29 |
| 404.47 | 2.75 | 2.75 |
| 404.47 | 2.75 | 2.75 |
| 404.47 | 3.82 | 3.82 |
| 407.45 | 3.18 | 3.18 |
| 404.47 | 2.75 | 2.75 |
| 404.47 | 2.75 | 2.75 |
| 404.47 | 3.82 | 3.82 |
| 407.45 | 3.18 | 3.18 |
| 458.44 | 3.46 | 3.46 |
| 458.44 | 3.46 | 3.46 |
| 458.44 | 4.53 | 4.53 |
| 461.42 | 3.89 | 3.89 |
| 441.50 | 2.99 | 2.99 |
| 441.49 | 4.06 | 4.06 |
| 444.47 | 3.42 | 3.42 |
| 419.49 | 2.45 | 2.45 |
| 419.49 | 2.45 | 2.45 |
| 419.49 | 3.52 | 3.52 |
| 422.46 | 2.89 | 2.89 |
| 432.32 | 3.71 | 3.71 |
| 397.48 | 3.35 | 3.35 |
| 370.42 | 3.69 | 3.69 |
| 441.50 | 2.99 | 2.99 |
| 392.44 | 2.91 | 2.91 |
| 421.48 | 2.92 | 2.92 |
| 461.42 | 3.32 | 3.32 |
| 460.44 | 3.93 | 3.93 |
| 472.47 | 3.77 | 3.77 |
| 429.41 | 2.93 | 2.93 |
| 444.42 | 2.64 | 2.64 |
| 444.42 | 2.64 | 2.64 |
| 443.43 | 3.24 | 3.24 |
| 457.43 | 4.55 | 4.55 |
| 454.46 | 4.12 | 4.12 |
| 428.42 | 3.54 | 3.54 |
| 407.45 | 2.67 | 2.67 |
| 407.45 | 2.67 | 2.67 |
| 407.45 | 2.67 | 2.67 |
| 411.47 | 3.07 | 3.07 |
| 426.48 | 2.77 | 2.77 |
| 403.49 | 3.59 | 3.59 |
| 437.51 | 3.65 | 3.65 |
| 394.87 | 3.17 | 3.17 |
| 434.89 | 3.92 | 3.92 |
| 465.36 | 3.86 | 3.86 |
| 454.33 | 2.99 | 2.99 |
| 429.41 | 2.93 | 2.93 |
| 458.42 | 3.95 | 3.95 |
| 456.47 | 4.90 | 4.90 |
| 459.43 | 2.94 | 2.94 |
| 459.43 | 2.94 | 2.94 |
| 458.42 | 3.95 | 3.95 |
| 477.49 | 4.92 | 4.92 |
| 443.43 | 3.24 | 3.24 |
| 457.46 | 4.30 | 4.30 |
| 444.42 | 2.64 | 2.64 |
| 470.46 | 3.82 | 3.82 |
| 447.40 | 3.07 | 3.07 |
| 495.41 | 3.54 | 3.54 |
| 462.41 | 3.85 | 3.85 |
| 459.43 | 2.94 | 2.94 |
| 447.40 | 3.07 | 3.07 |
| 447.40 | 3.07 | 3.07 |
| 447.40 | 3.07 | 3.07 |
| 447.39 | 4.15 | 4.15 |
| 458.42 | 3.95 | 3.95 |
| 454.46 | 4.12 | 4.12 |
| 458.42 | 3.95 | 3.95 |
| 443.43 | 4.32 | 4.32 |
| 458.42 | 3.95 | 3.95 |
| 447.40 | 3.07 | 3.07 |
| 458.42 | 3.95 | 3.95 |
| 443.43 | 3.24 | 3.24 |
| 454.46 | 4.12 | 4.12 |
| 502.28 | 4.27 | 4.27 |
| 404.45 | 3.24 | 3.24 |
| 457.43 | 4.55 | 4.55 |
| 454.46 | 4.12 | 4.12 |
| 443.43 | 4.32 | 4.32 |
| 447.39 | 4.15 | 4.15 |
| 430.39 | 2.33 | 2.33 |
| 470.46 | 3.82 | 3.82 |
| 458.42 | 3.95 | 3.95 |
| 457.46 | 4.30 | 4.30 |
| 453.47 | 4.72 | 4.72 |
| 446.41 | 3.68 | 3.68 |
| 442.45 | 3.85 | 3.85 |
| 442.44 | 4.92 | 4.92 |