Basic Information
ID: ALA3705845
Type: Binding
Description: Enzyme Assay: The procedure to determine CDA enzymatic activity is based on published methodologies (for example, Cacciamani, T. et al., Arch. Biochem. Biophys. 1991, 290, 285-92; Cohen R. et al., J. Biol. Chem., 1971, 246, 7566-8; Vincenzetti S. et al., Protein Expr. Purif. 1996, 8, 247-53). The assay follows the change in absorbance at 286 nm of the CDA-catalyzed deamination of cytidine to form uridine. The reaction is carried out in potassium phosphate buffer (pH 7.4, 20 mM, containing 1mM DTT) in a total volume of 200 μl in a 96-well plate format. The final reaction mixture contains cytidine (50 μM) and purified human recombinant CDA. Purified enzyme is diluted so as to produce an absorbance change of approximately 2 milli-absorbance units/minute. Base line measurements of absorbance change over time are made before CDA addition to insure no change of absorbance in the absence of CDA. After CDA addition, absorbance change is monitored for 20-30 minutes.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705845
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 268.22 | -1.57 | -1.57 |
| 250.23 | -1.86 | -1.86 |
| 268.22 | -1.57 | -1.57 |
| 250.23 | -1.86 | -1.86 |
| 250.23 | -1.86 | -1.86 |
| 250.23 | -1.86 | -1.86 |
| 268.22 | -1.57 | -1.57 |
| 250.23 | -1.86 | -1.86 |
| 250.23 | -1.86 | -1.86 |