Basic Information
ID: ALA3705863
Type: Binding
Description: Enzyme Inhibition Assay: Experimental Procedure for Kinetic Analyses: Enzymatic reactions were carried out in PBS buffer (pH 7.4) using pNP-GlcNAc as a substrate (0.5 mM) and monitored continuously at 37 C. at 400 nm using a Cary 3E UV-VIS spectrophotometer equipped with a Peltier temperature controller. Reactions were pre-heated in a 500 μL quartz cuvette for approximately 5 minutes followed by addition of 10 μL enzyme via syringe (final enzyme concentration 0.002 mg/mL). Reaction velocities were determined by linear regression of the linear region of the reaction progress curve between the first and third minutes.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705863
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 237.25 | -1.09 | -1.09 |
| 255.24 | -0.80 | -0.80 |
| 273.23 | -0.50 | -0.50 |