Basic Information
ID: ALA3705868
Type: Binding
Description: Biochemical Assay: The FASN enzyme was isolated from SKBr3 cells. SKBr3 is a human breast cancer cell-line with high levels of FASN expression. It is estimated that FASN comprises about 25% of the cytosolic proteins in this cell line. SKBr3 cells were homogenized in a dounce homogenizer then centrifuged for 15 minutes at 4C. to remove particulate matter. The supernatant was then analyzed for protein content, diluted to the appropriate concentration, and used to measure FASN activity. The presence of FASN was confirmed by western blot analysis. A similar method for isolation of FASN from SKBr3 cells is described in Teresa, P. et al. (Clin. Cancer Res. 2009; 15(24), 7608-7615).FASN activity of the SKBr3 cell extract was determined by measuring either NADPH oxidation or the amount of thiol-containing coenzyme A (CoA) released during the fatty acid synthase reaction. The dye CPM (7-diethylamino-3-(4â-maleimidyl-phenyl)-4-methylcoumarin) contains a thiol reactive group that increases its fluorescence emission.
Cell type: Cancer cell lines
Activity Charts
Bioactivity
Activity Types for Assay ALA3705868
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 493.61 | 5.13 | 5.13 |
| 522.65 | 5.22 | 5.22 |
| 504.64 | 5.73 | 5.73 |
| 513.62 | 4.54 | 4.54 |
| 513.62 | 4.54 | 4.54 |
| 490.61 | 5.34 | 5.34 |
| 490.61 | 5.34 | 5.34 |
| 492.58 | 4.01 | 4.01 |
| 519.65 | 4.58 | 4.58 |
| 533.68 | 4.89 | 4.89 |
| 519.65 | 4.54 | 4.54 |
| 520.64 | 4.97 | 4.97 |
| 490.61 | 5.43 | 5.43 |
| 476.58 | 5.12 | 5.12 |
| 490.61 | 5.43 | 5.43 |
| 421.50 | 4.82 | 4.82 |
| 420.52 | 5.43 | 5.43 |
| 426.52 | 4.35 | 4.35 |
| 481.60 | 4.49 | 4.49 |
| 439.56 | 4.23 | 4.23 |
| 467.57 | 4.18 | 4.18 |
| 503.63 | 3.59 | 3.59 |
| 545.71 | 4.68 | 4.68 |
| 458.54 | 4.80 | 4.80 |
| 546.70 | 3.75 | 3.75 |
| 483.53 | 5.80 | 5.80 |
| 495.63 | 4.44 | 4.44 |
| 458.54 | 4.74 | 4.74 |
| 449.98 | 5.44 | 5.44 |
| 481.60 | 4.05 | 4.05 |
| 440.55 | 4.66 | 4.66 |
| 499.53 | 5.68 | 5.68 |
| 490.61 | 5.34 | 5.34 |
| 508.60 | 5.42 | 5.42 |
| 509.59 | 4.82 | 4.82 |
| 455.95 | 5.48 | 5.48 |
| 506.61 | 5.13 | 5.13 |
| 501.59 | 4.99 | 4.99 |
| 511.03 | 5.77 | 5.77 |
| 560.58 | 6.02 | 6.02 |
| 464.57 | 5.34 | 5.34 |
| 441.54 | 4.18 | 4.18 |
| 415.50 | 4.07 | 4.07 |
| 495.63 | 5.74 | 5.74 |
| 513.62 | 5.74 | 5.74 |
| 541.63 | 4.76 | 4.76 |
| 543.64 | 5.01 | 5.01 |
| 554.67 | 4.68 | 4.68 |
| 511.60 | 5.13 | 5.13 |
| 526.62 | 4.78 | 4.78 |
| 527.64 | 6.00 | 6.00 |
| 499.59 | 5.44 | 5.44 |
| 482.59 | 4.75 | 4.75 |
| 482.59 | 4.75 | 4.75 |
| 469.55 | 3.84 | 3.84 |
| 481.56 | 3.53 | 3.53 |
| 499.55 | 3.62 | 3.62 |
| 511.60 | 5.08 | 5.08 |
| 503.58 | 4.36 | 4.36 |
| 517.99 | 5.17 | 5.17 |
| 555.65 | 5.01 | 5.01 |
| 557.67 | 5.26 | 5.26 |
| 543.60 | 3.55 | 3.55 |
| 557.63 | 3.80 | 3.80 |
| 556.64 | 4.41 | 4.41 |
| 478.57 | 4.58 | 4.58 |
| 478.57 | 4.58 | 4.58 |
| 464.54 | 4.19 | 4.19 |
| 492.60 | 4.83 | 4.83 |
| 492.60 | 4.83 | 4.83 |
| 464.54 | 4.27 | 4.27 |
| 464.54 | 4.27 | 4.27 |
| 448.54 | 5.03 | 5.03 |
| 451.50 | 3.27 | 3.27 |
| 450.51 | 3.88 | 3.88 |
| 466.51 | 3.58 | 3.58 |
| 454.48 | 3.71 | 3.71 |
| 464.54 | 4.13 | 4.13 |
| 504.48 | 4.59 | 4.59 |
| 464.54 | 4.27 | 4.27 |
| 438.50 | 3.74 | 3.74 |
| 465.53 | 4.61 | 4.61 |
| 506.58 | 4.45 | 4.45 |
| 451.50 | 4.31 | 4.31 |
| 510.64 | 4.62 | 4.62 |
| 486.62 | 4.49 | 4.49 |
| 433.53 | 4.93 | 4.93 |
| 493.63 | 4.19 | 4.19 |
| 445.56 | 4.79 | 4.79 |
| 524.59 | 5.64 | 5.64 |
| 507.64 | 4.88 | 4.88 |
| 474.58 | 4.76 | 4.76 |
| 456.55 | 4.36 | 4.36 |
| 538.69 | 4.95 | 4.95 |
| 502.63 | 5.39 | 5.39 |
| 415.54 | 4.79 | 4.79 |
| 497.60 | 4.71 | 4.71 |
| 495.63 | 4.88 | 4.88 |
| 509.65 | 5.13 | 5.13 |
| 429.56 | 5.09 | 5.09 |
| 438.58 | 5.46 | 5.46 |
| 440.55 | 4.66 | 4.66 |
| 446.94 | 4.69 | 4.69 |
| 467.62 | 5.04 | 5.04 |
| 483.57 | 4.40 | 4.40 |
| 496.58 | 5.72 | 5.72 |
| 522.63 | 5.81 | 5.81 |
| 494.57 | 5.17 | 5.17 |
| 478.55 | 5.44 | 5.44 |
| 435.53 | 5.13 | 5.13 |
| 505.62 | 5.44 | 5.44 |
| 477.57 | 4.84 | 4.84 |
| 459.55 | 5.61 | 5.61 |
| 491.60 | 5.25 | 5.25 |
| 465.56 | 5.14 | 5.14 |
| 451.53 | 4.43 | 4.43 |
| 507.59 | 4.91 | 4.91 |
| 504.64 | 5.73 | 5.73 |
| 484.60 | 4.96 | 4.96 |
| 435.52 | 3.40 | 3.40 |
| 481.60 | 5.16 | 5.16 |
| 522.65 | 4.41 | 4.41 |
| 480.57 | 4.22 | 4.22 |
| 405.50 | 3.75 | 3.75 |
| 403.49 | 3.60 | 3.60 |
| 513.62 | 5.74 | 5.74 |
| 443.55 | 4.63 | 4.63 |
| 415.50 | 3.82 | 3.82 |
| 401.47 | 3.51 | 3.51 |
| 469.59 | 4.56 | 4.56 |
| 443.55 | 4.17 | 4.17 |
| 429.52 | 4.13 | 4.13 |
| 455.56 | 4.83 | 4.83 |
| 442.52 | 3.34 | 3.34 |
| 456.55 | 3.68 | 3.68 |
| 455.56 | 4.83 | 4.83 |
| 469.59 | 4.87 | 4.87 |
| 469.59 | 4.70 | 4.70 |
| 424.51 | 4.05 | 4.05 |
| 438.54 | 4.44 | 4.44 |
| 455.56 | 4.17 | 4.17 |
| 482.63 | 4.65 | 4.65 |
| 469.59 | 4.74 | 4.74 |
| 468.61 | 4.61 | 4.61 |
| 480.62 | 4.75 | 4.75 |
| 496.62 | 4.18 | 4.18 |
| 457.58 | 4.42 | 4.42 |
| 475.57 | 4.50 | 4.50 |
| 475.57 | 4.85 | 4.85 |
| 469.59 | 4.74 | 4.74 |
| 489.60 | 5.24 | 5.24 |
| 439.56 | 5.25 | 5.25 |
| 483.62 | 5.13 | 5.13 |
| 469.59 | 5.09 | 5.09 |
| 455.56 | 4.43 | 4.43 |
| 501.61 | 5.21 | 5.21 |
| 526.60 | 6.28 | 6.28 |
| 457.55 | 5.33 | 5.33 |
| 482.55 | 6.39 | 6.39 |
| 453.59 | 5.50 | 5.50 |
| 471.58 | 5.58 | 5.58 |
| 467.62 | 6.06 | 6.06 |
| 485.61 | 6.14 | 6.14 |
| 493.53 | 5.96 | 5.96 |
| 475.54 | 5.88 | 5.88 |
| 493.53 | 5.96 | 5.96 |
| 457.55 | 5.27 | 5.27 |
| 439.56 | 5.19 | 5.19 |
| 443.53 | 5.02 | 5.02 |
| 487.58 | 4.90 | 4.90 |
| 495.63 | 5.79 | 5.79 |
| 513.62 | 5.87 | 5.87 |
| 495.63 | 5.79 | 5.79 |
| 453.59 | 5.64 | 5.64 |
| 467.62 | 5.89 | 5.89 |
| 463.97 | 4.51 | 4.51 |
| 478.00 | 4.77 | 4.77 |
| 490.01 | 5.08 | 5.08 |
| 461.54 | 4.25 | 4.25 |
| 481.64 | 6.14 | 6.14 |
| 483.62 | 4.99 | 4.99 |
| 469.59 | 4.95 | 4.95 |
| 501.61 | 5.42 | 5.42 |
| 483.62 | 5.34 | 5.34 |
| 485.61 | 5.84 | 5.84 |
| 515.63 | 5.46 | 5.46 |
| 497.64 | 5.38 | 5.38 |
| 467.62 | 5.58 | 5.58 |
| 455.56 | 4.70 | 4.70 |
| 457.58 | 4.42 | 4.42 |
| 469.59 | 4.74 | 4.74 |
| 471.61 | 4.98 | 4.98 |
| 443.55 | 4.38 | 4.38 |
| 469.59 | 5.09 | 5.09 |
| 457.58 | 4.94 | 4.94 |
| 457.58 | 4.77 | 4.77 |
| 471.61 | 4.98 | 4.98 |
| 457.58 | 4.94 | 4.94 |
| 457.58 | 4.68 | 4.68 |
| 443.55 | 4.63 | 4.63 |
| 425.54 | 4.94 | 4.94 |
| 443.53 | 5.02 | 5.02 |
| 457.58 | 4.89 | 4.89 |
| 429.52 | 4.38 | 4.38 |
| 459.55 | 4.01 | 4.01 |
| 471.56 | 4.15 | 4.15 |
| 443.55 | 4.50 | 4.50 |
| 471.61 | 4.68 | 4.68 |
| 483.62 | 4.99 | 4.99 |
| 484.60 | 4.07 | 4.07 |
| 472.59 | 4.06 | 4.06 |
| 470.58 | 3.82 | 3.82 |
| 454.58 | 4.58 | 4.58 |
| 483.62 | 5.59 | 5.59 |
| 469.59 | 5.20 | 5.20 |
| 487.58 | 5.28 | 5.28 |
| 501.61 | 5.67 | 5.67 |
| 469.59 | 5.34 | 5.34 |
| 487.58 | 5.42 | 5.42 |
| 530.57 | 6.57 | 6.57 |
| 455.56 | 4.95 | 4.95 |
| 473.55 | 5.03 | 5.03 |
| 498.55 | 6.09 | 6.09 |
| 516.54 | 6.18 | 6.18 |
| 473.55 | 4.89 | 4.89 |
| 487.58 | 5.28 | 5.28 |
| 469.59 | 5.20 | 5.20 |
| 455.56 | 4.81 | 4.81 |
| 454.57 | 5.77 | 5.77 |
| 489.57 | 5.75 | 5.75 |
| 455.56 | 4.29 | 4.29 |
| 497.60 | 4.06 | 4.06 |
| 402.49 | 4.85 | 4.85 |
| 452.60 | 6.11 | 6.11 |
| 456.57 | 5.93 | 5.93 |
| 500.62 | 5.81 | 5.81 |
| 482.63 | 5.73 | 5.73 |
| 486.59 | 5.77 | 5.77 |
| 468.60 | 5.69 | 5.69 |
| 424.55 | 5.54 | 5.54 |
| 438.58 | 5.85 | 5.85 |
| 486.59 | 5.51 | 5.51 |
| 468.60 | 5.42 | 5.42 |
| 452.60 | 6.11 | 6.11 |
| 468.60 | 5.69 | 5.69 |
| 486.59 | 5.77 | 5.77 |
| 500.62 | 6.08 | 6.08 |
| 452.60 | 6.16 | 6.16 |
| 438.58 | 5.85 | 5.85 |
| 492.55 | 6.56 | 6.56 |
| 458.99 | 6.20 | 6.20 |
| 449.56 | 5.42 | 5.42 |
| 415.54 | 4.51 | 4.51 |
| 459.59 | 4.53 | 4.53 |
| 429.56 | 4.90 | 4.90 |
| 454.57 | 5.44 | 5.44 |
| 445.59 | 4.96 | 4.96 |
| 498.63 | 5.27 | 5.27 |
| 431.54 | 3.80 | 3.80 |
| 417.51 | 3.41 | 3.41 |
| 479.58 | 4.83 | 4.83 |
| 444.58 | 3.62 | 3.62 |
| 458.61 | 3.96 | 3.96 |
| 431.54 | 3.65 | 3.65 |
| 469.59 | 4.66 | 4.66 |
| 497.60 | 3.96 | 3.96 |
| 495.58 | 3.71 | 3.71 |
| 522.65 | 4.29 | 4.29 |
| 494.60 | 4.25 | 4.25 |
| 413.53 | 4.80 | 4.80 |
| 399.50 | 4.23 | 4.23 |
| 456.55 | 3.87 | 3.87 |
| 456.55 | 3.87 | 3.87 |
| 474.54 | 3.95 | 3.95 |
| 470.58 | 4.18 | 4.18 |
| 464.53 | 5.08 | 5.08 |
| 436.52 | 4.90 | 4.90 |
| 428.54 | 4.87 | 4.87 |
| 464.53 | 5.08 | 5.08 |
| 415.50 | 4.58 | 4.58 |
| 436.52 | 4.90 | 4.90 |
| 385.47 | 4.35 | 4.35 |
| 479.59 | 4.97 | 4.97 |
| 493.62 | 5.72 | 5.72 |
| 481.60 | 5.16 | 5.16 |
| 479.58 | 4.92 | 4.92 |
| 479.58 | 4.92 | 4.92 |
| 467.57 | 4.77 | 4.77 |
| 522.65 | 4.41 | 4.41 |
| 414.51 | 4.31 | 4.31 |
| 436.53 | 4.75 | 4.75 |
| 408.46 | 2.14 | 2.14 |
| 480.57 | 4.14 | 4.14 |
| 406.49 | 3.15 | 3.15 |
| 405.50 | 3.81 | 3.81 |
| 417.51 | 3.96 | 3.96 |
| 431.54 | 4.35 | 4.35 |
| 447.54 | 3.58 | 3.58 |
| 497.57 | 4.83 | 4.83 |
| 433.51 | 3.19 | 3.19 |
| 498.56 | 4.22 | 4.22 |
| 451.50 | 3.27 | 3.27 |
| 451.50 | 3.27 | 3.27 |
| 465.53 | 3.66 | 3.66 |
| 450.51 | 3.88 | 3.88 |
| 450.51 | 3.88 | 3.88 |
| 437.48 | 2.88 | 2.88 |
| 470.93 | 4.22 | 4.22 |
| 498.56 | 4.22 | 4.22 |
| 500.58 | 4.83 | 4.83 |
| 456.52 | 3.41 | 3.41 |
| 491.61 | 4.43 | 4.43 |
| 455.56 | 4.27 | 4.27 |
| 477.58 | 4.09 | 4.09 |
| 501.54 | 4.66 | 4.66 |
| 452.53 | 4.08 | 4.08 |
| 551.54 | 5.54 | 5.54 |
| 513.57 | 4.53 | 4.53 |
| 464.54 | 4.27 | 4.27 |
| 540.64 | 4.33 | 4.33 |
| 478.57 | 4.44 | 4.44 |
| 451.50 | 4.31 | 4.31 |
| 492.55 | 4.14 | 4.14 |
| 492.60 | 4.83 | 4.83 |
| 451.50 | 4.31 | 4.31 |
| 525.63 | 5.39 | 5.39 |
| 557.67 | 5.26 | 5.26 |
| 527.64 | 6.00 | 6.00 |
| 542.62 | 4.16 | 4.16 |
| 479.55 | 4.87 | 4.87 |
| 479.55 | 4.87 | 4.87 |
| 521.62 | 4.78 | 4.78 |
| 491.60 | 5.32 | 5.32 |
| 494.60 | 4.53 | 4.53 |
| 482.59 | 4.75 | 4.75 |
| 482.59 | 4.75 | 4.75 |
| 408.51 | 3.09 | 3.09 |
| 446.55 | 4.31 | 4.31 |
| 444.54 | 4.16 | 4.16 |
| 448.52 | 3.30 | 3.30 |
| 509.61 | 5.00 | 5.00 |
| 462.55 | 3.64 | 3.64 |
| 550.66 | 3.93 | 3.93 |
| 536.64 | 3.59 | 3.59 |
| 539.64 | 4.26 | 4.26 |
| 525.61 | 4.23 | 4.23 |
| 511.58 | 3.58 | 3.58 |
| 525.61 | 3.60 | 3.60 |
| 474.56 | 3.83 | 3.83 |
| 447.54 | 3.70 | 3.70 |
| 487.60 | 3.74 | 3.74 |
| 522.61 | 4.17 | 4.17 |
| 474.56 | 3.83 | 3.83 |
| 510.60 | 4.39 | 4.39 |
| 426.52 | 4.48 | 4.48 |
| 430.51 | 3.62 | 3.62 |
| 428.54 | 4.63 | 4.63 |
| 419.53 | 4.06 | 4.06 |
| 421.50 | 3.05 | 3.05 |
| 482.59 | 4.75 | 4.75 |
| 473.58 | 3.40 | 3.40 |
| 488.59 | 4.03 | 4.03 |
| 433.51 | 3.19 | 3.19 |
| 433.51 | 3.19 | 3.19 |
| 485.59 | 4.03 | 4.03 |
| 471.57 | 3.78 | 3.78 |
| 457.54 | 3.77 | 3.77 |
| 408.51 | 3.09 | 3.09 |
| 394.48 | 2.84 | 2.84 |
| 394.48 | 2.84 | 2.84 |
| 485.59 | 4.03 | 4.03 |
| 480.61 | 5.62 | 5.62 |
| 454.57 | 4.91 | 4.91 |
| 454.57 | 4.91 | 4.91 |
| 472.56 | 4.99 | 4.99 |
| 486.59 | 5.25 | 5.25 |
| 509.65 | 5.00 | 5.00 |
| 495.63 | 4.74 | 4.74 |
| 513.62 | 4.82 | 4.82 |
| 468.60 | 5.47 | 5.47 |
| 466.59 | 5.23 | 5.23 |
| 480.61 | 5.48 | 5.48 |
| 523.64 | 5.28 | 5.28 |
| 507.64 | 5.06 | 5.06 |
| 498.60 | 5.70 | 5.70 |
| 493.66 | 5.53 | 5.53 |
| 511.65 | 5.61 | 5.61 |
| 439.56 | 4.26 | 4.26 |
| 537.66 | 5.67 | 5.67 |
| 555.65 | 5.75 | 5.75 |
| 537.66 | 5.53 | 5.53 |
| 493.66 | 5.40 | 5.40 |
| 466.59 | 5.31 | 5.31 |
| 484.58 | 5.39 | 5.39 |
| 498.60 | 5.95 | 5.95 |
| 466.59 | 5.48 | 5.48 |
| 479.63 | 5.22 | 5.22 |
| 497.62 | 5.30 | 5.30 |
| 523.64 | 5.36 | 5.36 |
| 541.63 | 5.44 | 5.44 |
| 537.66 | 5.75 | 5.75 |
| 509.61 | 4.97 | 4.97 |
| 495.63 | 4.34 | 4.34 |
| 479.63 | 5.11 | 5.11 |
| 453.59 | 4.57 | 4.57 |
| 467.62 | 4.96 | 4.96 |
| 485.61 | 4.91 | 4.91 |
| 503.60 | 4.99 | 4.99 |
| 495.63 | 4.53 | 4.53 |
| 511.63 | 4.75 | 4.75 |
| 495.67 | 5.39 | 5.39 |
| 481.64 | 5.35 | 5.35 |
| 507.68 | 5.92 | 5.92 |
| 454.57 | 4.70 | 4.70 |
| 454.57 | 5.05 | 5.05 |
| 499.63 | 4.96 | 4.96 |
| 511.65 | 5.35 | 5.35 |
| 525.67 | 5.66 | 5.66 |
| 493.66 | 5.27 | 5.27 |
| 507.68 | 5.57 | 5.57 |
| 513.01 | 5.18 | 5.18 |
| 505.99 | 5.44 | 5.44 |
| 490.58 | 4.84 | 4.84 |
| 490.58 | 4.84 | 4.84 |
| 452.60 | 6.11 | 6.11 |
| 425.54 | 4.94 | 4.94 |
| 457.60 | 5.39 | 5.39 |
| 468.60 | 5.39 | 5.39 |
| 454.57 | 5.22 | 5.22 |
| 509.65 | 5.22 | 5.22 |
| 509.70 | 6.09 | 6.09 |
| 511.63 | 5.10 | 5.10 |
| 511.63 | 5.27 | 5.27 |
| 467.62 | 4.96 | 4.96 |
| 467.62 | 5.13 | 5.13 |
| 495.63 | 5.05 | 5.05 |
| 507.64 | 4.98 | 4.98 |
| 468.60 | 5.09 | 5.09 |
| 454.57 | 4.43 | 4.43 |
| 454.57 | 4.70 | 4.70 |
| 468.60 | 5.44 | 5.44 |
| 454.57 | 5.22 | 5.22 |
| 454.57 | 5.05 | 5.05 |
| 449.56 | 4.82 | 4.82 |
| 426.52 | 4.62 | 4.62 |
| 444.51 | 4.49 | 4.49 |
| 502.02 | 4.83 | 4.83 |
| 495.00 | 5.10 | 5.10 |
| 476.53 | 4.82 | 4.82 |
| 478.96 | 5.08 | 5.08 |
| 492.98 | 5.34 | 5.34 |
| 516.05 | 5.09 | 5.09 |
| 474.56 | 3.83 | 3.83 |
| 436.49 | 3.49 | 3.49 |
| 429.52 | 3.86 | 3.86 |
| 487.58 | 5.17 | 5.17 |
| 455.56 | 4.70 | 4.70 |
| 464.56 | 6.31 | 6.31 |
| 415.50 | 3.82 | 3.82 |
| 454.57 | 5.38 | 5.38 |
| 442.54 | 5.63 | 5.63 |
| 487.58 | 4.56 | 4.56 |
| 454.58 | 4.58 | 4.58 |
| 437.57 | 4.22 | 4.22 |
| 401.51 | 4.80 | 4.80 |
| 472.59 | 4.76 | 4.76 |
| 472.56 | 5.46 | 5.46 |
| 473.57 | 5.18 | 5.18 |
| 473.57 | 5.18 | 5.18 |
| 486.62 | 5.15 | 5.15 |
| 486.62 | 5.15 | 5.15 |
| 463.56 | 3.38 | 3.38 |