Basic Information
ID: ALA3705917
Type: Binding
Description: In vitro Competitive Displacement Binding Assays : Modified SMAC peptides and compounds were tested for their ability to displace the fluorescent tracer from either XIAP, clAP-1 or clAP-2. BIR3 domains of clAP-1 , clAP-2 and XIAP were incubated with test compounds or SMAC based peptides and their respective peptide probes (Peptide Protein Research) in assay buffer (50mM Hepes pH 7.5, 0.025% Tween-20, 0.01 % BSA, and 1 mM DTT). Positive controls consisted of BIR3 proteins and tracer (no inhibition) and negative controls contained tracer only (100% inhibition). The samples were incubated at room temperature for 1 hr (XIAP and clAP-2) or 3hrs (clAP-1 ) prior to being read in the BMG Pherastar in Fluorescence Polarization mode (FP 485nm, 520nm, 520nm).
Format: BAO_0000357
Target: Inhibitor of apoptosis protein 3(ALA4198)
Document: ALA3639218
BindingDB Source: 6885
Activity Charts
Bioactivity
Activity Types for Assay ALA3705917
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 422.57 | 2.61 | 2.61 |
| 293.80 | 1.52 | 1.52 |
| 327.35 | 1.89 | 1.89 |
| 337.45 | 0.27 | 0.27 |
| 294.79 | 0.92 | 0.92 |
| 321.85 | 2.26 | 2.26 |
| 365.90 | 2.27 | 2.27 |
| 364.49 | 2.64 | 2.64 |
| 277.34 | 1.01 | 1.01 |
| 330.43 | 0.57 | 0.57 |
| 353.44 | 2.68 | 2.68 |
| 431.36 | 0.54 | 0.54 |
| 445.38 | 0.88 | 0.88 |
| 352.46 | -0.22 | -0.22 |
| 343.35 | 1.77 | 1.77 |
| 349.48 | 2.46 | 2.46 |
| 366.49 | 0.12 | 0.12 |
| 350.47 | 1.86 | 1.86 |
| 388.87 | 2.72 | 2.72 |
| 351.88 | 1.62 | 1.62 |
| 417.51 | 1.84 | 1.84 |
| 407.56 | 2.56 | 2.56 |
| 356.30 | 2.91 | 2.91 |
| 445.56 | 2.58 | 2.58 |
| 473.61 | 3.36 | 3.36 |
| 438.57 | 1.53 | 1.53 |
| 411.54 | 1.93 | 1.93 |
| 319.84 | 2.01 | 2.01 |
| 378.90 | 1.37 | 1.37 |
| 502.61 | 1.69 | 1.69 |
| 451.63 | 2.05 | 2.05 |
| 362.47 | 1.02 | 1.02 |
| 438.57 | 1.83 | 1.83 |
| 388.56 | 2.21 | 2.21 |
| 424.55 | 2.81 | 2.81 |
| 476.62 | 2.03 | 2.03 |
| 440.56 | 2.74 | 2.74 |
| 447.58 | 2.48 | 2.48 |
| 457.02 | 3.26 | 3.26 |
| 440.56 | 2.74 | 2.74 |
| 398.55 | 2.58 | 2.58 |
| 406.57 | 3.37 | 3.37 |
| 396.54 | 2.02 | 2.02 |
| 491.64 | 1.82 | 1.82 |
| 415.54 | 1.14 | 1.14 |
| 357.46 | 0.89 | 0.89 |
| 412.58 | 2.97 | 2.97 |
| 442.00 | 3.50 | 3.50 |
| 434.97 | 1.49 | 1.49 |
| 458.99 | 3.46 | 3.46 |
| 396.51 | 2.54 | 2.54 |
| 414.59 | 3.06 | 3.06 |
| 428.53 | 3.22 | 3.22 |
| 412.54 | 1.42 | 1.42 |
| 460.53 | 3.09 | 3.09 |
| 454.37 | 1.54 | 1.54 |
| 452.60 | 2.75 | 2.75 |
| 489.66 | 2.97 | 2.97 |
| 463.02 | 2.27 | 2.27 |
| 418.54 | 2.52 | 2.52 |
| 422.57 | 2.61 | 2.61 |
| 418.54 | 2.52 | 2.52 |
| 418.54 | 2.52 | 2.52 |
| 447.97 | 2.63 | 2.63 |
| 476.02 | 3.28 | 3.28 |
| 458.55 | 3.15 | 3.15 |
| 487.60 | 1.73 | 1.73 |
| 487.60 | 1.73 | 1.73 |
| 454.58 | 2.77 | 2.77 |
| 485.58 | 2.60 | 2.60 |
| 491.64 | 1.82 | 1.82 |
| 480.62 | 2.81 | 2.81 |
| 312.42 | 1.48 | 1.48 |
| 485.58 | 2.33 | 2.33 |
| 474.55 | 2.12 | 2.12 |
| 501.58 | 1.56 | 1.56 |
| 440.38 | 2.01 | 2.01 |
| 449.60 | 2.05 | 2.05 |
| 488.63 | 3.54 | 3.54 |
| 513.02 | 3.85 | 3.85 |
| 396.48 | 2.13 | 2.13 |
| 522.60 | 2.49 | 2.49 |
| 449.55 | 2.10 | 2.10 |
| 463.57 | 2.49 | 2.49 |
| 525.64 | 3.25 | 3.25 |
| 522.60 | 2.49 | 2.49 |
| 326.44 | 1.86 | 1.86 |
| 515.62 | 3.28 | 3.28 |
| 513.59 | 2.69 | 2.69 |
| 475.58 | 2.49 | 2.49 |
| 513.63 | 2.57 | 2.57 |
| 515.62 | 3.55 | 3.55 |
| 484.60 | 2.94 | 2.94 |
| 491.63 | 2.59 | 2.59 |
| 477.63 | 3.96 | 3.96 |
| 495.64 | 2.43 | 2.43 |
| 479.62 | 2.59 | 2.59 |
| 497.63 | 3.14 | 3.14 |
| 479.62 | 2.59 | 2.59 |
| 509.64 | 2.22 | 2.22 |