Basic Information
ID: ALA3705951
Type: Binding
Description: Inhibition Assay: Jurkat cells were placed in a 96 well plate (0.5 million cells per well in 1% FBS medium) then a test compound of this invention was added at different concentrations. After 10 minutes, the cells were activated with PHA (final concentration 2.5 μg/mL) and incubated for 20 hours at 37° C. under CO2. The final volume was 200 μL. Following incubation, the cells were centrifuged and the supernatants collected and stored at −70° C. prior to assaying for IL-2 production. A commercial ELISA kit (IL-2 Eli-pair, Diaclone Research, Besancon, France) was used to detect production of IL-2, from which dose response curves were obtained.
Cell type: Jurkat
Activity Charts
Bioactivity
Activity Types for Assay ALA3705951
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 417.79 | 6.62 | 6.62 |
| 396.82 | 6.04 | 6.04 |
| 387.41 | 5.04 | 5.04 |
| 366.44 | 4.47 | 4.47 |
| 383.34 | 5.96 | 5.96 |
| 387.41 | 5.04 | 5.04 |
| 396.42 | 5.91 | 5.91 |
| 387.41 | 5.04 | 5.04 |
| 382.39 | 5.60 | 5.60 |