Basic Information
ID: ALA3705957
Type: Binding
Description: Enzyme Inhibition Assay: Enzyme activity is measured by observing the increase in fluorescence intensity caused by cleavage of a peptide substrate containing a fluorophore whose emission is quenched in the intact peptide.Assay buffer: 100 mM potassium phosphate pH 6.5, EDTA-Na 5 mM, Triton X-100 0.001%, DTT 5 mM.Enzymes (all at 1 nM): human and mouse Cathepsin S, Cat K, Cat B, Cat L.Substrate (20 μM): Z-Val-Val-Arg-AMC, except for Cat K which uses Z-Leu-Arg-AMC (both from Bachem).Z=Benzyloxycarbonyl.AMC=7-Amino-4-Methyl-Coumarin.DTT=dithiothreitol.Final volume: 100 μL.Excitation 360 nm, Emission 465 nm.Enzyme is added to the substance dilutions in 96-well microtitre plates and the reaction is started with substrate. Fluorescence emission is measured over 20 minutes, during which time a linear increase is observed in the absence of inhibitor.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705957
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 678.60 | 3.50 | 3.50 |
| 578.48 | 1.85 | 1.85 |
| 592.51 | 2.20 | 2.20 |
| 746.59 | 4.43 | 4.43 |
| 791.04 | 4.54 | 4.54 |
| 646.48 | 2.79 | 2.79 |
| 690.93 | 2.90 | 2.90 |
| 710.51 | 3.76 | 3.76 |
| 754.96 | 3.87 | 3.87 |
| 666.56 | 3.63 | 3.63 |
| 711.01 | 3.74 | 3.74 |
| 566.44 | 1.98 | 1.98 |
| 610.89 | 2.09 | 2.09 |
| 624.92 | 2.44 | 2.44 |
| 638.95 | 2.83 | 2.83 |
| 708.92 | 3.03 | 3.03 |
| 664.47 | 2.93 | 2.93 |
| 684.55 | 4.12 | 4.12 |
| 729.00 | 4.23 | 4.23 |
| 778.61 | 5.31 | 5.31 |
| 823.06 | 5.42 | 5.42 |
| 584.43 | 2.47 | 2.47 |
| 628.88 | 2.58 | 2.58 |
| 823.06 | 5.07 | 5.07 |
| 722.94 | 3.42 | 3.42 |
| 678.49 | 3.66 | 3.66 |
| 722.94 | 3.77 | 3.77 |
| 736.97 | 3.77 | 3.77 |
| 757.67 | 5.35 | 5.35 |
| 802.12 | 5.46 | 5.46 |
| 657.56 | 3.70 | 3.70 |
| 702.01 | 3.81 | 3.81 |
| 746.65 | 4.38 | 4.38 |
| 791.10 | 4.49 | 4.49 |
| 646.53 | 2.73 | 2.73 |
| 690.98 | 2.84 | 2.84 |
| 786.98 | 4.40 | 4.40 |
| 750.95 | 3.29 | 3.29 |
| 780.98 | 3.90 | 3.90 |
| 804.97 | 4.70 | 4.70 |