Basic Information
ID: ALA3705965
Type: Binding
Description: Spectrophotometric 384 Well Assay: Malonyl CoA formation by acetyl CoA carboxylases is stoichometrically linked to the consumption of ATP. ACC2 activity is measured in a NADH-linked kinetic method measuring ADP generated during the ACC reaction using a coupled lactate dehydrogenase/pyruvate kinase reaction. For biological testing, a human ACC2 construct which lacks the 128 amino acids at the N-terminus for increased solubility (nt 385-6966 in Genbank entry AJ575592) is cloned. The protein is then expressed in insect cells using a baculoviral expression system. Protein purification is performed by anion exchange.
Format: BAO_0000219
Organism: Homo sapiens
Target: Acetyl-CoA carboxylase 2(ALA4829)
Document: ALA3638991
BindingDB Source: 6168
Activity Charts
Bioactivity
Activity Types for Assay ALA3705965
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 402.45 | 3.31 | 3.31 |
| 366.51 | 4.15 | 4.15 |
| 378.52 | 4.29 | 4.29 |
| 364.49 | 3.90 | 3.90 |
| 378.52 | 4.15 | 4.15 |
| 367.49 | 3.54 | 3.54 |
| 380.49 | 3.08 | 3.08 |
| 379.51 | 3.11 | 3.11 |
| 352.48 | 3.76 | 3.76 |
| 364.53 | 4.66 | 4.66 |
| 359.47 | 3.91 | 3.91 |
| 396.53 | 4.15 | 4.15 |
| 367.49 | 3.54 | 3.54 |
| 366.46 | 3.13 | 3.13 |
| 380.49 | 3.52 | 3.52 |
| 378.52 | 4.29 | 4.29 |
| 366.50 | 4.15 | 4.15 |
| 392.54 | 4.54 | 4.54 |
| 380.53 | 4.53 | 4.53 |
| 338.45 | 3.89 | 3.89 |
| 352.43 | 3.26 | 3.26 |
| 378.52 | 4.81 | 4.81 |
| 367.50 | 3.49 | 3.49 |
| 364.49 | 4.28 | 4.28 |
| 364.49 | 4.42 | 4.42 |
| 350.46 | 4.03 | 4.03 |
| 352.48 | 4.28 | 4.28 |
| 352.43 | 3.26 | 3.26 |
| 338.45 | 3.89 | 3.89 |
| 366.46 | 3.65 | 3.65 |
| 433.47 | 3.93 | 3.93 |
| 401.46 | 3.92 | 3.92 |
| 381.48 | 2.11 | 2.11 |
| 371.46 | 3.62 | 3.62 |
| 419.45 | 4.06 | 4.06 |
| 389.30 | 2.91 | 2.91 |
| 352.48 | 3.10 | 3.10 |
| 420.94 | 4.47 | 4.47 |
| 364.49 | 3.42 | 3.42 |
| 441.54 | 4.35 | 4.35 |
| 391.44 | 4.16 | 4.16 |
| 413.48 | 4.09 | 4.09 |
| 402.50 | 3.95 | 3.95 |
| 363.38 | 3.90 | 3.90 |
| 363.38 | 3.90 | 3.90 |
| 378.50 | 4.01 | 4.01 |
| 336.44 | 3.15 | 3.15 |
| 419.51 | 4.15 | 4.15 |
| 416.47 | 3.18 | 3.18 |
| 364.37 | 3.30 | 3.30 |
| 431.54 | 4.87 | 4.87 |
| 416.53 | 4.29 | 4.29 |
| 416.57 | 5.22 | 5.22 |
| 504.66 | 5.29 | 5.29 |
| 498.63 | 4.79 | 4.79 |
| 404.55 | 4.70 | 4.70 |
| 429.56 | 4.43 | 4.43 |
| 406.57 | 4.93 | 4.93 |
| 410.53 | 4.48 | 4.48 |
| 392.54 | 4.54 | 4.54 |
| 428.52 | 4.78 | 4.78 |
| 414.50 | 4.39 | 4.39 |
| 443.59 | 4.70 | 4.70 |
| 396.51 | 4.10 | 4.10 |
| 518.68 | 5.16 | 5.16 |
| 408.54 | 3.77 | 3.77 |
| 445.56 | 4.74 | 4.74 |
| 417.55 | 4.43 | 4.43 |
| 392.50 | 3.68 | 3.68 |
| 420.55 | 4.29 | 4.29 |
| 429.56 | 4.83 | 4.83 |
| 408.54 | 4.29 | 4.29 |
| 434.58 | 4.68 | 4.68 |
| 441.58 | 4.89 | 4.89 |
| 447.60 | 5.34 | 5.34 |
| 404.55 | 5.06 | 5.06 |
| 434.58 | 4.83 | 4.83 |
| 403.53 | 4.42 | 4.42 |
| 431.54 | 3.55 | 3.55 |
| 408.54 | 4.29 | 4.29 |
| 490.63 | 4.99 | 4.99 |
| 392.54 | 4.91 | 4.91 |
| 410.49 | 3.99 | 3.99 |
| 433.55 | 3.39 | 3.39 |
| 415.54 | 4.56 | 4.56 |
| 483.61 | 5.53 | 5.53 |
| 422.54 | 5.00 | 5.00 |
| 461.63 | 5.64 | 5.64 |
| 433.58 | 5.03 | 5.03 |
| 447.60 | 5.34 | 5.34 |
| 449.60 | 4.17 | 4.17 |
| 378.52 | 4.67 | 4.67 |
| 430.55 | 5.47 | 5.47 |
| 405.45 | 3.54 | 3.54 |
| 417.55 | 4.81 | 4.81 |
| 410.53 | 5.00 | 5.00 |
| 446.51 | 5.45 | 5.45 |
| 442.56 | 4.29 | 4.29 |
| 490.63 | 4.99 | 4.99 |
| 394.52 | 3.90 | 3.90 |