Inhibition Assay: Thoroughly mixed test compounds, enzyme and reaction buffer, preincubated the mixture for 15 minutes at 37° C. and then primed react...

Basic Information

ID: ALA3705997

Type: Binding

Description: Inhibition Assay: Thoroughly mixed test compounds, enzyme and reaction buffer, preincubated the mixture for 15 minutes at 37° C. and then primed reaction by adding substrate, successfully detecting fluorescence value at 460 nm for 5 minutes. At the same time, set the blank control group without substrate and solvent control group with DMSO replacing test compound, as well as positive control group of Vildagliptin (LAF-237) and Sitagliptin (MK-0431) [Bioorg. Med. Chem. Lett., 2005, 15, 4770-4773]. All final reaction volumes were 100 μL. Each concentration of each sample consisted of parallel wells in triplate.

Format: BAO_0000357

Organism: Rattus norvegicus

Target: Dipeptidyl peptidase IV(ALA4653)

Document: ALA3638422

BindingDB Source: 6404

Associated Items: Associated Bioactivities Associated Compounds

Activity Charts

Bioactivity

Activity Types for Assay ALA3705997

IC50

Total(9)

Parent Molecular Weight	ALogP	Polar Surface Area
407.32	2.02	2.02
341.38	1.53	1.53
398.43	1.58	1.58
415.43	2.17	2.17
395.43	1.83	1.83
420.44	1.73	1.73
411.47	2.47	2.47
509.55	2.14	2.14
303.41	1.17	1.17

Parent Molecular Weight

ALogP

Polar Surface Area

407.32

2.02

341.38

1.53

398.43

1.58

415.43

2.17

395.43

1.83

420.44

1.73

411.47

2.47

509.55

2.14

303.41

1.17

Inhibition Assay: Thoroughly mixed test compounds, enzyme and reaction buffer, preincubated the mixture for 15 minutes at 37° C. and then primed react...

Basic Information

Activity Charts

Compound Summary