Basic Information
ID: ALA3706031
Type: Binding
Description: Kinase Assay: Approximately 5-20 μg of purified GST-RET proteins were incubated with 1 mM ATP in 20 μL kinase buffer (10 mM Tris-HCl, 5 mM MgCl2, pH 7.4), at 30° C. for 30 minutes. The kinase reactions were terminated by boiling the samples in SDS-PAGE sample buffer. Samples were resolved on 10% acrylamide gels by SDS-PAGE followed by Western blotting. Phosphorylation was detected using a pan-phosphotyrosine pY99 antibody (available from Santa Cruz Biotechnology) or RET phospho-specific antibody (pY905, available from Cell Signaling, Beverly, Mass., USA).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706031
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 263.38 | 3.83 | 3.83 |
| 293.41 | 4.18 | 4.18 |
| 277.41 | 4.17 | 4.17 |
| 275.40 | 4.55 | 4.55 |
| 279.38 | 3.96 | 3.96 |
| 223.32 | 2.88 | 2.88 |
| 418.97 | 5.78 | 5.78 |
| 291.44 | 4.71 | 4.71 |
| 293.41 | 3.75 | 3.75 |
| 279.38 | 3.84 | 3.84 |
| 363.50 | 4.97 | 4.97 |
| 252.36 | 2.91 | 2.91 |
| 280.42 | 3.43 | 3.43 |