Basic Information
ID: ALA3706065
Type: Binding
Description: Radioligand Binding Assay: Radioligand binding assays were performed in CHO cells expressing human CCR2B and Galpha±16 coupling protein. All compounds were dissolved in DMSO and assays run at a final DMSO concentration of 0.5% (v/v). [125I]-labeled human MCP-1 was purchased from PerkinElmer. Unlabelled human MCP-1 was purchased from PeproTech.Compounds were serially diluted in DMSO before diluting into assay buffer (25 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.5% BSA) with cryo preserved CHO cells expressing human CCR2B and the Galpha±16 coupling protein (30x103/well) and [125I]-MCP-1 (50 pM). The reaction was incubated at room temperature for 90 minutes before transferring to GF/C filter membrane (PerkinElmer) pre-treated with 0.3% polyethyleneimine for 2 hours at 4 C. The filter membrane was washed six times with ice cold wash buffer (25 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 500 mM NaCl, 0.01% (m/v) azide), dried, and sealed in a RLB sample bag (Agilent Technologies).
Format: BAO_0000219
Cell type: CHO
Target: C-C chemokine receptor type 2(ALA4015)
Document: ALA3638389
BindingDB Source: 6587
Activity Charts
Bioactivity
Activity Types for Assay ALA3706065
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 508.59 | 3.23 | 3.23 |
| 508.59 | 3.23 | 3.23 |
| 508.59 | 3.23 | 3.23 |
| 508.59 | 3.23 | 3.23 |
| 509.57 | 2.63 | 2.63 |
| 509.57 | 2.63 | 2.63 |
| 522.61 | 3.57 | 3.57 |
| 522.61 | 3.62 | 3.62 |
| 582.67 | 5.42 | 5.42 |
| 582.67 | 5.42 | 5.42 |
| 522.61 | 3.54 | 3.54 |
| 509.57 | 2.63 | 2.63 |
| 509.57 | 2.63 | 2.63 |
| 525.59 | 3.98 | 3.98 |
| 525.59 | 3.98 | 3.98 |
| 521.62 | 4.15 | 4.15 |
| 525.59 | 3.98 | 3.98 |
| 507.60 | 3.84 | 3.84 |
| 521.62 | 4.15 | 4.15 |
| 492.04 | 3.61 | 3.61 |
| 526.58 | 3.37 | 3.37 |
| 508.59 | 3.23 | 3.23 |
| 521.62 | 4.15 | 4.15 |
| 521.62 | 4.15 | 4.15 |
| 525.59 | 3.98 | 3.98 |
| 508.59 | 3.23 | 3.23 |
| 509.57 | 2.63 | 2.63 |
| 532.61 | 3.71 | 3.71 |
| 523.60 | 3.72 | 3.72 |
| 514.61 | 3.29 | 3.29 |
| 537.62 | 3.85 | 3.85 |
| 543.03 | 3.89 | 3.89 |
| 508.59 | 3.23 | 3.23 |
| 508.59 | 3.23 | 3.23 |
| 475.03 | 2.87 | 2.87 |
| 532.61 | 3.71 | 3.71 |
| 532.61 | 3.71 | 3.71 |
| 522.61 | 3.54 | 3.54 |
| 532.61 | 3.71 | 3.71 |
| 508.49 | 4.13 | 4.13 |
| 522.61 | 3.62 | 3.62 |
| 532.61 | 3.71 | 3.71 |
| 575.59 | 4.86 | 4.86 |
| 499.06 | 3.34 | 3.34 |
| 532.61 | 3.71 | 3.71 |
| 542.04 | 4.49 | 4.49 |
| 499.06 | 3.34 | 3.34 |
| 562.68 | 6.20 | 6.20 |
| 538.66 | 5.72 | 5.72 |
| 575.59 | 4.86 | 4.86 |
| 556.07 | 4.83 | 4.83 |
| 589.62 | 5.20 | 5.20 |
| 546.63 | 4.05 | 4.05 |
| 546.63 | 4.05 | 4.05 |
| 589.62 | 5.20 | 5.20 |
| 546.63 | 4.05 | 4.05 |
| 546.63 | 4.05 | 4.05 |
| 513.08 | 3.69 | 3.69 |
| 464.53 | 3.17 | 3.17 |
| 463.62 | 2.55 | 2.55 |
| 492.59 | 3.85 | 3.85 |
| 492.59 | 3.85 | 3.85 |
| 492.59 | 3.85 | 3.85 |
| 492.59 | 3.85 | 3.85 |
| 506.61 | 4.24 | 4.24 |
| 506.61 | 4.24 | 4.24 |
| 479.59 | 4.41 | 4.41 |
| 465.56 | 4.07 | 4.07 |