Basic Information
ID: ALA3706076
Type: Binding
Description: Binding Assay: Membranes from CHO/Galpha16 cells stably transfected with human hMCH-1R are resuspended using a syringe (needle 0.6x25 mm) and diluted in test buffer (50 mM HEPES, 10 mM MgCl2, 2 mM EGTA, pH 7.00; 0.1% bovine serum albumin (protease-free), 0.021% bacitracin, 1 ug/ml aprotinin, 1 ug/ml leupeptin and 1 uM phosphoramidone) to a concentration of 5 to 15 ug/ml. 200 microliters of this membrane fraction (contains 1 to 3 ug of protein) are incubated for 60 minutes at ambient temperature with 100 pM of 125I-tyrosyl melanin concentrating hormone (125I-MCH commercially obtainable from NEN) and increasing concentrations of the test compound in a final volume of 250 microliters. After the incubation the reaction is filtered using a cell harvester through 0.5% PEI treated fibreglass filters (GF/B, Unifilter Packard). The membrane-bound radioactivity retained on the filter is then determined after the addition of scintillator substance (Packard Microscint 20).
Format: BAO_0000219
Organism: Homo sapiens
Cell type: CHO/Galpha16
Target: Melanin-concentrating hormone receptor 1(ALA344)
Document: ALA3638848
BindingDB Source: 6631
Activity Charts
Bioactivity
Activity Types for Assay ALA3706076
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 390.48 | 3.68 | 3.68 |
| 425.92 | 3.73 | 3.73 |
| 470.37 | 3.84 | 3.84 |
| 409.46 | 3.21 | 3.21 |
| 391.47 | 3.08 | 3.08 |
| 426.90 | 3.12 | 3.12 |
| 471.36 | 3.23 | 3.23 |
| 418.49 | 2.80 | 2.80 |
| 446.55 | 3.58 | 3.58 |
| 416.52 | 4.21 | 4.21 |
| 446.55 | 3.58 | 3.58 |
| 435.50 | 3.75 | 3.75 |
| 495.02 | 3.80 | 3.80 |
| 540.46 | 4.12 | 4.12 |
| 447.54 | 2.97 | 2.97 |
| 436.49 | 3.14 | 3.14 |
| 482.97 | 3.02 | 3.02 |
| 467.96 | 2.47 | 2.47 |
| 513.39 | 2.74 | 2.74 |
| 376.46 | 3.34 | 3.34 |
| 432.52 | 3.45 | 3.45 |
| 445.56 | 3.37 | 3.37 |
| 461.56 | 2.69 | 2.69 |
| 430.55 | 4.60 | 4.60 |
| 432.52 | 3.19 | 3.19 |
| 390.48 | 3.73 | 3.73 |
| 446.55 | 3.58 | 3.58 |
| 432.52 | 3.19 | 3.19 |
| 446.55 | 3.58 | 3.58 |
| 460.57 | 3.97 | 3.97 |
| 446.55 | 3.58 | 3.58 |
| 458.56 | 3.43 | 3.43 |
| 449.53 | 4.14 | 4.14 |
| 465.53 | 3.11 | 3.11 |
| 451.50 | 2.72 | 2.72 |
| 409.46 | 3.26 | 3.26 |
| 481.98 | 3.62 | 3.62 |
| 467.95 | 3.23 | 3.23 |
| 467.95 | 3.23 | 3.23 |
| 481.00 | 3.41 | 3.41 |
| 465.98 | 4.65 | 4.65 |
| 481.98 | 3.62 | 3.62 |
| 451.95 | 4.26 | 4.26 |
| 411.89 | 3.39 | 3.39 |
| 425.92 | 3.78 | 3.78 |
| 496.01 | 3.87 | 3.87 |
| 481.98 | 3.62 | 3.62 |
| 496.01 | 4.01 | 4.01 |
| 453.93 | 2.84 | 2.84 |
| 526.43 | 3.73 | 3.73 |
| 540.46 | 3.98 | 3.98 |
| 526.43 | 3.73 | 3.73 |
| 526.43 | 3.73 | 3.73 |
| 470.37 | 3.89 | 3.89 |
| 496.41 | 4.37 | 4.37 |
| 512.40 | 3.34 | 3.34 |
| 539.47 | 3.91 | 3.91 |
| 512.40 | 3.61 | 3.61 |
| 526.43 | 3.73 | 3.73 |
| 456.34 | 3.50 | 3.50 |
| 525.45 | 3.52 | 3.52 |
| 484.39 | 4.27 | 4.27 |
| 510.43 | 4.76 | 4.76 |
| 498.42 | 4.62 | 4.62 |
| 484.39 | 4.23 | 4.23 |
| 511.42 | 4.16 | 4.16 |
| 499.41 | 4.01 | 4.01 |
| 483.37 | 3.38 | 3.38 |
| 447.54 | 2.97 | 2.97 |
| 433.51 | 2.58 | 2.58 |
| 433.51 | 2.58 | 2.58 |
| 391.47 | 3.12 | 3.12 |
| 419.48 | 2.19 | 2.19 |
| 447.54 | 2.97 | 2.97 |
| 447.54 | 3.23 | 3.23 |
| 462.55 | 2.52 | 2.52 |
| 461.56 | 3.22 | 3.22 |
| 461.56 | 3.36 | 3.36 |
| 447.54 | 2.97 | 2.97 |
| 447.54 | 2.97 | 2.97 |
| 450.51 | 3.53 | 3.53 |
| 410.45 | 2.66 | 2.66 |
| 466.51 | 2.50 | 2.50 |
| 410.45 | 2.61 | 2.61 |
| 452.49 | 2.11 | 2.11 |
| 496.01 | 3.20 | 3.20 |
| 454.91 | 2.24 | 2.24 |
| 468.94 | 2.63 | 2.63 |
| 466.97 | 4.05 | 4.05 |
| 412.88 | 2.78 | 2.78 |
| 426.90 | 3.17 | 3.17 |
| 497.00 | 3.27 | 3.27 |
| 468.94 | 2.63 | 2.63 |
| 482.97 | 3.02 | 3.02 |
| 482.97 | 3.02 | 3.02 |
| 497.00 | 3.41 | 3.41 |
| 452.94 | 3.66 | 3.66 |
| 499.37 | 2.35 | 2.35 |
| 481.98 | 2.81 | 2.81 |