Basic Information
ID: ALA3706090
Type: Binding
Description: Inhibition Assay: Zero point five μL of the test compounds (dissolved in N,N′-dimethylsulfoxide) were incubated with 48.5 μL of the fluorescence-quenched peptide substrate solution (Biotin-XSEVNLDAEFRHDSGC-Eu: X=εe-amino-n-capronic acid,Eu=Europium cryptate) and 1 μL of recombinant human BACE-1 protein (R&D systems) for 3 h at 30° C. in the 96 well half-area plate (black color plate,Costar). The substrate peptide was synthesized by reacting with Biotin-XSEVNLDAEFRHDSGC (Peptide Institute) and Cryptate TBPCOOH mono SMP (CIS bio international). The final concentration of the substrate peptide and recombinant human BACE-1 protein were 18 nM and 7.4 nM, respectively. The enzymatic reaction was performed in sodium acetate buffer (50 mM sodium acetate (pH 5.0), 0.008% Triton X-100). After the reaction, a 50 μL of 8.0 μg/mL Streptavidin-XL665 (CIS bio international) dissolved in phosphate buffer (150 mM K2HPO4KH2PO4 (pH 7.0), 0.008% Triton X-100, 0.8 M KF) was added.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706090
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 505.76 | 7.79 | 7.79 |
| 304.36 | 3.20 | 3.20 |
| 398.47 | 4.01 | 4.01 |
| 348.20 | 3.12 | 3.12 |
| 358.43 | 3.83 | 3.83 |
| 239.32 | 3.61 | 3.61 |
| 342.90 | 5.18 | 5.18 |
| 439.77 | 4.42 | 4.42 |
| 389.91 | 4.02 | 4.02 |
| 371.46 | 3.08 | 3.08 |
| 422.35 | 4.58 | 4.58 |
| 410.36 | 4.43 | 4.43 |
| 386.91 | 4.05 | 4.05 |
| 461.41 | 4.98 | 4.98 |
| 343.88 | 4.57 | 4.57 |
| 385.49 | 3.38 | 3.38 |
| 345.90 | 4.62 | 4.62 |
| 394.29 | 3.96 | 3.96 |
| 394.48 | 4.29 | 4.29 |
| 462.42 | 5.04 | 5.04 |
| 400.45 | 4.37 | 4.37 |
| 387.48 | 3.82 | 3.82 |
| 340.49 | 4.37 | 4.37 |
| 395.28 | 3.36 | 3.36 |
| 405.32 | 3.76 | 3.76 |
| 373.45 | 3.51 | 3.51 |
| 374.94 | 5.02 | 5.02 |
| 483.23 | 5.13 | 5.13 |
| 360.87 | 3.65 | 3.65 |
| 460.42 | 5.58 | 5.58 |
| 428.87 | 4.67 | 4.67 |
| 360.87 | 3.65 | 3.65 |
| 394.33 | 4.91 | 4.91 |
| 350.45 | 3.48 | 3.48 |
| 448.39 | 4.51 | 4.51 |
| 403.94 | 4.41 | 4.41 |
| 387.48 | 3.89 | 3.89 |
| 437.49 | 4.77 | 4.77 |
| 453.49 | 4.65 | 4.65 |
| 359.45 | 3.35 | 3.35 |
| 359.52 | 3.91 | 3.91 |
| 438.78 | 5.02 | 5.02 |
| 416.96 | 4.87 | 4.87 |
| 349.84 | 3.85 | 3.85 |
| 395.32 | 4.31 | 4.31 |
| 415.97 | 5.47 | 5.47 |
| 405.32 | 3.76 | 3.76 |
| 346.84 | 3.48 | 3.48 |
| 422.35 | 4.58 | 4.58 |
| 378.86 | 3.79 | 3.79 |
| 395.32 | 4.31 | 4.31 |
| 369.45 | 2.10 | 2.10 |
| 395.32 | 4.31 | 4.31 |
| 341.44 | 2.70 | 2.70 |
| 418.95 | 4.44 | 4.44 |
| 390.90 | 3.66 | 3.66 |
| 322.41 | 3.44 | 3.44 |
| 411.55 | 3.01 | 3.01 |
| 304.42 | 3.14 | 3.14 |
| 385.92 | 4.87 | 4.87 |