Basic Information
ID: ALA3706106
Type: Binding
Description: FLIPR Calcium Assay: Ca2+ influx was measured using a FLIPR calcium assay kit (R8033; Molecular Devices, Sunnyvale, Calif.). The Ca2+ indicator dye was dissolved in Hanks' balanced salt solution supplemented with 20 mM HEPES buffer (HBSS/HEPES) according to the manufacturer instructions. Prior to start of the assay, the medium was removed by aspiration, and cells were loaded with 100 μL Ca2+ dye for 2 to 3 hours at room temperature. AITC (allyl isothiocyanate) was used to activate and open the channels. (4×) solutions of the test compounds were prepared in HBSS/HEPES, and 50 μL were added to the cells at a delivery rate of 10 μL/sec. Changes in fluorescence were measured over time in a fluorometric imaging plate reader (FLIPR, Molecular Devices). Two additions were made over the course of an experimental run. For agonist experiments, assay buffer was added at the 10 s time point, followed by addition of agonist at the 3 minute 10 sec time point.
Activity Charts
Bioactivity
Activity Types for Assay ALA3706106
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 207.25 | 3.47 | 3.47 |
| 221.28 | 3.64 | 3.64 |
| 207.25 | 3.47 | 3.47 |
| 221.28 | 3.86 | 3.86 |
| 235.30 | 4.11 | 4.11 |
| 235.30 | 4.25 | 4.25 |
| 221.28 | 3.72 | 3.72 |
| 221.28 | 3.72 | 3.72 |
| 241.69 | 4.12 | 4.12 |
| 207.25 | 3.47 | 3.47 |
| 179.19 | 2.69 | 2.69 |
| 207.25 | 3.47 | 3.47 |
| 193.22 | 3.08 | 3.08 |
| 225.24 | 3.61 | 3.61 |
| 243.23 | 3.75 | 3.75 |
| 221.28 | 3.86 | 3.86 |
| 221.27 | 3.78 | 3.78 |
| 275.24 | 4.49 | 4.49 |
| 232.26 | 3.34 | 3.34 |