ID: ALA3706110
Type: Binding
Description: FLIPR Assay: Ca2+ Flux: 1321N1 cells expressing the recombinant human, rat or mouse P2X7 channel was cultured in HyQ DME/(HyClone/Dulbecco's Modified Eagle Medium) high glucose supplemented with 10% Fetal Bovine Serum (FBS) and appropriate selection marker. Cells were seeded at a density of 25000 cells/well (96-well clear bottom black walled plates) in 100 ul volume/well. On the day of the experiment, cell plates were washed with assay buffer, containing (in mM): 130 NaCl, 2 KCl, 1 CaCl2, 1 MgCl2, 10 HEPES, 5 glucose; pH 7.40 and 300 mOs. After the wash, cells were loaded with the Calcium-4 dye (Molecular Device) and incubated in the dark for 60 minutes. Test compounds were prepared at 250x the test concentration in neat DMSO. Intermediate 96-well compound plates were prepared by transferring 1.2 uL of the compound into 300 uL of assay buffer. A further 3x dilution occurred when transferring 50 uL/well of the compound plate to 100 uL/well in the cell plate.
Format: BAO_0000219
Cell type: 1321-N1
Target: P2X purinoceptor 7(ALA4805)
Document: ALA3638702
BindingDB Source: 6928
Bioactivity
Activity Types for Assay ALA3706110
IC50
Parent Molecular Weight | ALogP | Polar Surface Area |
---|---|---|
424.79 | 4.41 | 4.41 |
375.22 | 2.70 | 2.70 |
408.77 | 3.06 | 3.06 |
422.80 | 3.62 | 3.62 |
375.22 | 2.70 | 2.70 |
342.31 | 1.67 | 1.67 |
418.87 | 1.45 | 1.45 |
374.23 | 3.30 | 3.30 |
407.78 | 3.67 | 3.67 |
389.25 | 3.09 | 3.09 |
389.25 | 3.09 | 3.09 |
389.25 | 3.09 | 3.09 |
422.80 | 3.45 | 3.45 |
422.80 | 3.45 | 3.45 |
422.80 | 3.45 | 3.45 |
438.81 | 4.80 | 4.80 |
421.81 | 4.06 | 4.06 |
436.83 | 3.76 | 3.76 |
411.77 | 3.65 | 3.65 |
411.77 | 3.65 | 3.65 |
411.77 | 3.65 | 3.65 |
424.81 | 3.40 | 3.40 |
424.81 | 3.40 | 3.40 |
410.79 | 3.38 | 3.38 |
424.81 | 3.69 | 3.69 |
410.79 | 3.38 | 3.38 |
421.81 | 4.06 | 4.06 |
439.80 | 4.20 | 4.20 |
427.84 | 4.12 | 4.12 |
427.84 | 4.12 | 4.12 |
422.80 | 3.45 | 3.45 |
440.79 | 3.59 | 3.59 |
451.84 | 3.55 | 3.55 |
441.87 | 4.43 | 4.43 |
427.84 | 4.12 | 4.12 |
495.84 | 5.14 | 5.14 |
455.89 | 4.74 | 4.74 |
441.87 | 4.43 | 4.43 |
411.77 | 3.65 | 3.65 |
501.69 | 4.21 | 4.21 |
422.80 | 3.45 | 3.45 |
439.80 | 4.20 | 4.20 |
435.84 | 4.37 | 4.37 |
421.81 | 4.06 | 4.06 |
435.84 | 4.37 | 4.37 |
398.82 | 4.26 | 4.26 |
384.79 | 3.87 | 3.87 |
426.87 | 5.04 | 5.04 |
456.26 | 4.71 | 4.71 |
428.84 | 3.89 | 3.89 |
490.80 | 4.47 | 4.47 |
489.81 | 5.08 | 5.08 |
456.26 | 4.71 | 4.71 |
435.84 | 4.37 | 4.37 |
513.95 | 4.72 | 4.72 |
527.98 | 5.11 | 5.11 |
427.86 | 3.46 | 3.46 |
448.84 | 3.84 | 3.84 |
448.84 | 3.84 | 3.84 |
448.84 | 3.84 | 3.84 |
448.84 | 3.84 | 3.84 |
448.84 | 3.84 | 3.84 |
448.84 | 3.84 | 3.84 |
447.85 | 4.45 | 4.45 |
436.83 | 3.78 | 3.78 |
465.84 | 4.59 | 4.59 |
466.83 | 3.98 | 3.98 |
436.83 | 3.84 | 3.84 |
435.84 | 4.45 | 4.45 |
424.81 | 3.78 | 3.78 |
454.82 | 3.98 | 3.98 |
453.83 | 4.59 | 4.59 |
372.79 | 2.57 | 2.57 |
320.36 | 1.78 | 1.78 |
354.80 | 2.43 | 2.43 |
432.89 | 1.84 | 1.84 |
404.35 | 2.68 | 2.68 |
398.81 | 2.16 | 2.16 |
402.38 | 3.11 | 3.11 |
389.25 | 3.09 | 3.09 |
348.41 | 2.40 | 2.40 |
388.35 | 2.80 | 2.80 |
365.35 | 1.69 | 1.69 |
399.80 | 2.34 | 2.34 |
369.82 | 2.02 | 2.02 |
334.38 | 2.34 | 2.34 |
436.83 | 4.01 | 4.01 |
334.38 | 2.34 | 2.34 |
334.38 | 2.34 | 2.34 |
422.80 | 3.45 | 3.45 |