Basic Information
ID: ALA3706111
Type: Binding
Description: Radioligand Binding Assay: Radioligand binding: human or rat P2X7-1321N1 cells were collected and frozen @ −80° C. On the day of the experiment, cell membrane preparations were made according to standard published methods. The total assay volume was 100 μl:10 μl compound (10×)+(b) 40 μl tracer (2.5×)+50 μl membrane (2×). The tracer used for the assay was tritiated A-804598. The compound can be prepared as described in the literature. (Donnelly-Roberts, D. Neuropharmacology 2009, 56 (1), 223-229.) Compounds, tracer and membranes were incubated for 1 hour @ 4° C. The assay was terminated by filtration (GF/B filters pre-soaked with 0.3% PEI) and washed with washing buffer (Tris-HCl 50 mM).
Format: BAO_0000219
Cell type: P2X7-1321N1
Target: P2X purinoceptor 7(ALA4805)
Document: ALA3638702
BindingDB Source: 6928
Activity Charts
Bioactivity
Activity Types for Assay ALA3706111
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 424.79 | 4.41 | 4.41 |
| 375.22 | 2.70 | 2.70 |
| 408.77 | 3.06 | 3.06 |
| 374.23 | 3.30 | 3.30 |
| 407.78 | 3.67 | 3.67 |
| 389.25 | 3.09 | 3.09 |
| 389.25 | 3.09 | 3.09 |
| 389.25 | 3.09 | 3.09 |
| 422.80 | 3.45 | 3.45 |
| 422.80 | 3.45 | 3.45 |
| 422.80 | 3.45 | 3.45 |
| 438.81 | 4.80 | 4.80 |
| 421.81 | 4.06 | 4.06 |
| 436.83 | 3.76 | 3.76 |
| 411.77 | 3.65 | 3.65 |
| 411.77 | 3.65 | 3.65 |
| 411.77 | 3.65 | 3.65 |
| 424.81 | 3.40 | 3.40 |
| 424.81 | 3.40 | 3.40 |
| 410.79 | 3.38 | 3.38 |
| 424.81 | 3.69 | 3.69 |
| 410.79 | 3.38 | 3.38 |
| 421.81 | 4.06 | 4.06 |
| 439.80 | 4.20 | 4.20 |
| 427.84 | 4.12 | 4.12 |
| 427.84 | 4.12 | 4.12 |
| 422.80 | 3.45 | 3.45 |
| 440.79 | 3.59 | 3.59 |
| 451.84 | 3.55 | 3.55 |
| 441.87 | 4.43 | 4.43 |
| 427.84 | 4.12 | 4.12 |
| 455.89 | 4.74 | 4.74 |
| 441.87 | 4.43 | 4.43 |
| 411.77 | 3.65 | 3.65 |
| 501.69 | 4.21 | 4.21 |
| 439.80 | 4.20 | 4.20 |
| 435.84 | 4.37 | 4.37 |
| 421.81 | 4.06 | 4.06 |
| 435.84 | 4.37 | 4.37 |
| 456.26 | 4.71 | 4.71 |
| 490.80 | 4.47 | 4.47 |
| 489.81 | 5.08 | 5.08 |
| 456.26 | 4.71 | 4.71 |
| 435.84 | 4.37 | 4.37 |
| 513.95 | 4.72 | 4.72 |
| 427.86 | 3.46 | 3.46 |
| 448.84 | 3.84 | 3.84 |
| 448.84 | 3.84 | 3.84 |
| 448.84 | 3.84 | 3.84 |
| 448.84 | 3.84 | 3.84 |
| 448.84 | 3.84 | 3.84 |
| 447.85 | 4.45 | 4.45 |
| 436.83 | 3.78 | 3.78 |
| 465.84 | 4.59 | 4.59 |
| 466.83 | 3.98 | 3.98 |
| 436.83 | 3.84 | 3.84 |
| 435.84 | 4.45 | 4.45 |
| 424.81 | 3.78 | 3.78 |
| 454.82 | 3.98 | 3.98 |
| 453.83 | 4.59 | 4.59 |
| 372.79 | 2.57 | 2.57 |
| 354.80 | 2.43 | 2.43 |
| 432.89 | 1.84 | 1.84 |
| 402.38 | 3.11 | 3.11 |
| 389.25 | 3.09 | 3.09 |
| 348.41 | 2.40 | 2.40 |
| 388.35 | 2.80 | 2.80 |
| 334.38 | 2.34 | 2.34 |
| 436.83 | 4.01 | 4.01 |
| 334.38 | 2.34 | 2.34 |
| 422.80 | 3.45 | 3.45 |