Basic Information
ID: ALA3706135
Type: Binding
Description: ADP-Glo Kinase Assay: In this assay, the inhibitory activity of a test substance against the tyrosine kinase activity of FGFR1 protein is measured.To each well of a flat bottom 96 well white plate (Sumitomo Bakelite Co., Ltd., MS-8496W), 10 ul of FGFR1 protein (Cama Biosciences, Inc., 08-133) solution diluted to 1 ug/mL with an assay buffer (20 mM HEPES NaOH, 0.01% Triton X-100, 2 mM DTT, and 5 mM MgCl2), 10 ul, of an assay buffer solution containing CSK-tide substrate (Ana Spec Inc., 63843) in a final concentration of 1000 nM and ATP (Promega Corporation, V9102) in a final concentration of 58.3 uM, and 5 ul of a test substance diluted with the assay buffer were added, and the reaction was performed at room temperature for 1 hour (kinase reaction). For measuring kinase activity, ADP-Glo Kinase Assay (Promega Corporation, V9102) was used. After the reaction, 25 uL of ADP-Glo reagent was added to each well of the plate, and the reaction was performed at morn temperature for 40 minutes.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706135
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 383.45 | 2.92 | 2.92 |
| 527.63 | 5.48 | 5.48 |
| 543.62 | 4.45 | 4.45 |
| 557.65 | 4.84 | 4.84 |
| 559.64 | 5.82 | 5.82 |
| 517.56 | 4.69 | 4.69 |
| 517.56 | 4.83 | 4.83 |
| 568.68 | 5.34 | 5.34 |
| 529.60 | 4.23 | 4.23 |
| 545.60 | 3.47 | 3.47 |
| 556.67 | 4.46 | 4.46 |
| 559.62 | 4.11 | 4.11 |
| 517.59 | 4.13 | 4.13 |
| 559.62 | 4.11 | 4.11 |
| 513.60 | 5.13 | 5.13 |
| 541.65 | 5.87 | 5.87 |
| 527.63 | 5.52 | 5.52 |
| 585.71 | 5.62 | 5.62 |
| 565.58 | 4.83 | 4.83 |
| 543.62 | 4.76 | 4.76 |
| 557.65 | 5.10 | 5.10 |
| 587.68 | 4.47 | 4.47 |
| 585.71 | 5.88 | 5.88 |
| 557.65 | 5.15 | 5.15 |
| 601.70 | 4.86 | 4.86 |
| 557.65 | 5.10 | 5.10 |
| 573.65 | 4.08 | 4.08 |
| 586.69 | 5.28 | 5.28 |
| 587.68 | 4.64 | 4.64 |
| 600.72 | 4.87 | 4.87 |
| 579.68 | 4.31 | 4.31 |
| 543.62 | 4.71 | 4.71 |
| 573.67 | 6.21 | 6.21 |
| 535.63 | 4.30 | 4.30 |
| 535.63 | 4.30 | 4.30 |
| 548.67 | 4.52 | 4.52 |
| 548.67 | 4.48 | 4.48 |
| 535.63 | 4.30 | 4.30 |
| 573.65 | 4.50 | 4.50 |
| 543.62 | 5.14 | 5.14 |
| 547.62 | 3.49 | 3.49 |
| 547.62 | 3.49 | 3.49 |
| 531.62 | 4.52 | 4.52 |
| 529.60 | 4.23 | 4.23 |
| 529.60 | 4.23 | 4.23 |
| 515.57 | 3.84 | 3.84 |
| 515.57 | 3.84 | 3.84 |
| 557.65 | 4.84 | 4.84 |
| 571.68 | 5.23 | 5.23 |
| 541.65 | 5.86 | 5.86 |
| 573.65 | 4.50 | 4.50 |
| 513.60 | 5.08 | 5.08 |
| 543.62 | 4.45 | 4.45 |
| 499.57 | 4.70 | 4.70 |
| 572.67 | 4.29 | 4.29 |
| 543.62 | 4.28 | 4.28 |
| 513.60 | 5.09 | 5.09 |
| 585.71 | 5.62 | 5.62 |
| 555.68 | 6.25 | 6.25 |
| 571.68 | 5.23 | 5.23 |
| 571.68 | 5.23 | 5.23 |
| 582.70 | 5.73 | 5.73 |
| 557.65 | 4.84 | 4.84 |
| 527.63 | 5.48 | 5.48 |
| 543.62 | 4.62 | 4.62 |
| 585.71 | 5.62 | 5.62 |
| 571.68 | 5.23 | 5.23 |
| 555.68 | 6.25 | 6.25 |
| 541.65 | 5.86 | 5.86 |
| 557.65 | 5.01 | 5.01 |
| 561.61 | 4.57 | 4.57 |
| 561.61 | 4.57 | 4.57 |
| 575.64 | 4.96 | 4.96 |
| 603.70 | 5.57 | 5.57 |
| 603.70 | 5.57 | 5.57 |
| 559.64 | 5.82 | 5.82 |
| 586.69 | 5.28 | 5.28 |
| 572.67 | 4.89 | 4.89 |
| 587.68 | 4.46 | 4.46 |
| 573.65 | 4.07 | 4.07 |
| 573.65 | 4.07 | 4.07 |
| 579.68 | 4.31 | 4.31 |
| 579.68 | 4.31 | 4.31 |
| 565.65 | 3.92 | 3.92 |
| 565.65 | 3.92 | 3.92 |
| 579.68 | 4.31 | 4.31 |
| 529.60 | 4.32 | 4.32 |
| 557.65 | 5.10 | 5.10 |
| 543.62 | 4.71 | 4.71 |
| 601.70 | 4.85 | 4.85 |
| 601.70 | 4.85 | 4.85 |
| 571.68 | 5.49 | 5.49 |
| 573.65 | 4.25 | 4.25 |
| 600.72 | 5.67 | 5.67 |
| 601.70 | 4.85 | 4.85 |
| 587.68 | 4.46 | 4.46 |
| 587.68 | 4.46 | 4.46 |
| 571.68 | 5.49 | 5.49 |
| 587.68 | 4.64 | 4.64 |
| 573.65 | 4.25 | 4.25 |
| 599.73 | 6.27 | 6.27 |
| 615.73 | 5.24 | 5.24 |
| 615.73 | 5.24 | 5.24 |
| 585.71 | 5.88 | 5.88 |
| 593.71 | 4.70 | 4.70 |
| 593.71 | 4.70 | 4.70 |
| 593.71 | 4.70 | 4.70 |
| 573.65 | 4.25 | 4.25 |
| 589.67 | 5.62 | 5.62 |
| 600.72 | 4.82 | 4.82 |
| 629.76 | 5.63 | 5.63 |
| 571.68 | 5.49 | 5.49 |
| 587.68 | 4.64 | 4.64 |
| 587.68 | 4.64 | 4.64 |
| 587.68 | 4.64 | 4.64 |
| 587.68 | 4.46 | 4.46 |
| 587.68 | 4.46 | 4.46 |
| 573.65 | 4.25 | 4.25 |
| 573.65 | 4.25 | 4.25 |
| 587.68 | 4.64 | 4.64 |
| 571.68 | 5.49 | 5.49 |
| 557.65 | 5.10 | 5.10 |
| 615.73 | 5.24 | 5.24 |
| 615.73 | 5.24 | 5.24 |
| 599.73 | 6.27 | 6.27 |
| 601.70 | 4.86 | 4.86 |
| 585.71 | 5.88 | 5.88 |
| 571.68 | 5.49 | 5.49 |