Basic Information
ID: ALA3706152
Type: Binding
Description: Enzyme Inhibition Assay: An IMAP TR-FRET assay was used to analyze the enzyme activity (Molecular Devices Corp., Sunnyvale Calif.). 5 μL of serial diluted PDE10A (BPS Bioscience, San Diego, Calif.) or tissue homogenate was incubated with equal volumes of diluted fluorescein labeled cAMP or cGMP for 60 min in 384-well polystyrene assay plates (Corning, Corning, N.Y.) at room temperature. After incubation, the reaction was stopped by adding 60 μL of diluted binding reagents and was incubated for 3 hours to overnight at room temperature. The plates were read on an Envision (Perkin Elmer, Waltham, Mass.) for time resolved fluorescence resonance energy transfer.
Organism: Rattus norvegicus
Activity Charts
Bioactivity
Activity Types for Assay ALA3706152
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 432.53 | 4.58 | 4.58 |
| 349.39 | 3.24 | 3.24 |
| 331.30 | 5.03 | 5.03 |
| 345.32 | 5.34 | 5.34 |
| 365.74 | 5.68 | 5.68 |
| 363.31 | 5.48 | 5.48 |
| 399.29 | 6.05 | 6.05 |
| 403.40 | 6.21 | 6.21 |
| 361.32 | 5.04 | 5.04 |
| 399.29 | 6.05 | 6.05 |
| 365.74 | 5.68 | 5.68 |
| 345.32 | 5.34 | 5.34 |
| 430.43 | 4.86 | 4.86 |
| 332.29 | 4.43 | 4.43 |
| 335.38 | 5.34 | 5.34 |
| 374.83 | 5.73 | 5.73 |
| 358.38 | 5.21 | 5.21 |
| 408.38 | 6.09 | 6.09 |
| 264.29 | 2.91 | 2.91 |
| 365.40 | 4.95 | 4.95 |
| 407.40 | 6.70 | 6.70 |
| 364.41 | 5.55 | 5.55 |
| 357.39 | 5.82 | 5.82 |
| 407.40 | 6.70 | 6.70 |
| 353.43 | 5.99 | 5.99 |
| 372.40 | 5.52 | 5.52 |
| 341.37 | 4.47 | 4.47 |
| 409.37 | 5.49 | 5.49 |
| 359.36 | 4.61 | 4.61 |
| 409.37 | 5.49 | 5.49 |
| 409.37 | 5.49 | 5.49 |
| 366.38 | 4.34 | 4.34 |
| 339.40 | 5.68 | 5.68 |
| 376.46 | 4.63 | 4.63 |
| 376.46 | 4.49 | 4.49 |
| 348.41 | 3.58 | 3.58 |
| 348.41 | 3.58 | 3.58 |
| 371.44 | 4.75 | 4.75 |
| 371.44 | 4.75 | 4.75 |
| 362.43 | 3.83 | 3.83 |
| 362.43 | 3.97 | 3.97 |
| 357.42 | 4.36 | 4.36 |
| 362.43 | 4.24 | 4.24 |
| 414.47 | 4.70 | 4.70 |
| 346.43 | 5.00 | 5.00 |
| 362.43 | 3.97 | 3.97 |
| 362.43 | 3.97 | 3.97 |
| 376.46 | 4.63 | 4.63 |
| 404.47 | 4.40 | 4.40 |
| 404.51 | 5.00 | 5.00 |
| 390.49 | 4.88 | 4.88 |
| 360.42 | 4.18 | 4.18 |
| 390.44 | 4.01 | 4.01 |
| 361.45 | 3.76 | 3.76 |
| 391.48 | 3.13 | 3.13 |
| 447.54 | 5.12 | 5.12 |
| 376.46 | 4.22 | 4.22 |
| 418.50 | 4.79 | 4.79 |
| 403.49 | 3.97 | 3.97 |
| 355.40 | 5.15 | 5.15 |
| 412.45 | 5.54 | 5.54 |
| 376.46 | 4.22 | 4.22 |
| 405.50 | 4.39 | 4.39 |
| 363.42 | 3.37 | 3.37 |
| 363.42 | 3.37 | 3.37 |
| 333.39 | 4.01 | 4.01 |
| 377.45 | 4.02 | 4.02 |
| 377.45 | 4.02 | 4.02 |
| 363.42 | 3.37 | 3.37 |
| 363.38 | 2.93 | 2.93 |
| 455.52 | 4.99 | 4.99 |
| 455.52 | 4.99 | 4.99 |
| 377.45 | 4.02 | 4.02 |
| 363.42 | 3.37 | 3.37 |
| 377.45 | 3.62 | 3.62 |
| 391.48 | 4.27 | 4.27 |
| 377.45 | 3.76 | 3.76 |
| 391.43 | 3.41 | 3.41 |
| 319.37 | 3.98 | 3.98 |
| 333.40 | 4.23 | 4.23 |
| 307.36 | 3.84 | 3.84 |
| 321.38 | 4.23 | 4.23 |
| 369.43 | 5.02 | 5.02 |
| 370.42 | 4.41 | 4.41 |
| 370.42 | 4.41 | 4.41 |
| 370.42 | 4.41 | 4.41 |
| 383.45 | 5.06 | 5.06 |
| 384.44 | 4.46 | 4.46 |
| 384.44 | 4.46 | 4.46 |
| 384.44 | 4.46 | 4.46 |
| 363.42 | 4.00 | 4.00 |
| 335.41 | 4.48 | 4.48 |
| 349.39 | 3.61 | 3.61 |
| 390.49 | 4.31 | 4.31 |
| 476.58 | 5.47 | 5.47 |
| 337.38 | 3.47 | 3.47 |
| 376.46 | 3.82 | 3.82 |
| 323.36 | 2.81 | 2.81 |
| 347.42 | 4.40 | 4.40 |
| 349.39 | 2.98 | 2.98 |
| 362.44 | 3.16 | 3.16 |
| 348.41 | 2.82 | 2.82 |
| 377.45 | 4.02 | 4.02 |
| 349.39 | 2.98 | 2.98 |
| 363.42 | 3.23 | 3.23 |
| 363.42 | 3.23 | 3.23 |
| 363.42 | 3.63 | 3.63 |
| 404.47 | 3.47 | 3.47 |
| 437.55 | 5.87 | 5.87 |
| 370.41 | 5.08 | 5.08 |
| 370.41 | 5.08 | 5.08 |
| 364.41 | 6.23 | 6.23 |
| 369.42 | 5.69 | 5.69 |
| 355.40 | 5.39 | 5.39 |
| 355.40 | 5.39 | 5.39 |
| 369.42 | 5.17 | 5.17 |
| 383.41 | 5.38 | 5.38 |
| 358.38 | 5.21 | 5.21 |
| 368.44 | 5.14 | 5.14 |
| 364.41 | 5.55 | 5.55 |
| 444.54 | 6.04 | 6.04 |
| 329.36 | 4.40 | 4.40 |
| 370.41 | 5.08 | 5.08 |
| 341.37 | 4.47 | 4.47 |
| 370.41 | 5.08 | 5.08 |
| 369.42 | 5.69 | 5.69 |
| 365.40 | 4.95 | 4.95 |
| 358.38 | 5.21 | 5.21 |
| 385.49 | 6.40 | 6.40 |
| 385.49 | 6.40 | 6.40 |
| 353.43 | 5.99 | 5.99 |
| 357.39 | 5.82 | 5.82 |
| 375.38 | 5.96 | 5.96 |
| 375.38 | 5.96 | 5.96 |
| 345.43 | 5.74 | 5.74 |
| 387.41 | 5.83 | 5.83 |
| 343.43 | 5.97 | 5.97 |
| 345.45 | 6.06 | 6.06 |
| 329.40 | 5.58 | 5.58 |
| 331.42 | 5.67 | 5.67 |
| 388.40 | 5.22 | 5.22 |
| 376.37 | 5.35 | 5.35 |
| 390.45 | 6.23 | 6.23 |
| 356.39 | 4.05 | 4.05 |
| 390.45 | 6.23 | 6.23 |
| 393.45 | 5.57 | 5.57 |
| 373.42 | 3.92 | 3.92 |
| 390.45 | 6.23 | 6.23 |
| 414.47 | 6.70 | 6.70 |
| 372.40 | 5.52 | 5.52 |
| 400.46 | 6.08 | 6.08 |
| 331.38 | 4.01 | 4.01 |
| 329.40 | 5.02 | 5.02 |
| 345.40 | 4.13 | 4.13 |
| 331.38 | 3.74 | 3.74 |
| 377.35 | 4.75 | 4.75 |
| 389.39 | 4.62 | 4.62 |
| 344.38 | 3.81 | 3.81 |
| 355.40 | 4.66 | 4.66 |
| 375.82 | 5.12 | 5.12 |
| 366.38 | 5.02 | 5.02 |
| 365.40 | 4.95 | 4.95 |
| 365.40 | 4.95 | 4.95 |
| 359.36 | 4.61 | 4.61 |
| 355.40 | 4.66 | 4.66 |
| 340.39 | 5.07 | 5.07 |
| 341.37 | 4.47 | 4.47 |
| 341.37 | 4.47 | 4.47 |
| 370.41 | 4.57 | 4.57 |
| 391.43 | 5.62 | 5.62 |
| 374.83 | 5.73 | 5.73 |
| 394.44 | 4.96 | 4.96 |
| 390.45 | 6.23 | 6.23 |
| 357.38 | 3.45 | 3.45 |
| 372.39 | 3.87 | 3.87 |
| 425.88 | 6.28 | 6.28 |
| 413.49 | 4.20 | 4.20 |
| 396.48 | 6.29 | 6.29 |
| 344.42 | 5.36 | 5.36 |
| 330.39 | 4.97 | 4.97 |
| 354.37 | 3.96 | 3.96 |
| 355.36 | 3.35 | 3.35 |
| 383.40 | 5.18 | 5.18 |
| 381.86 | 5.56 | 5.56 |
| 348.41 | 4.30 | 4.30 |
| 366.40 | 4.44 | 4.44 |
| 376.46 | 4.86 | 4.86 |
| 378.43 | 4.31 | 4.31 |
| 331.38 | 4.64 | 4.64 |
| 334.38 | 3.91 | 3.91 |
| 264.29 | 3.41 | 3.41 |
| 361.45 | 4.89 | 4.89 |
| 361.45 | 4.89 | 4.89 |
| 406.46 | 4.88 | 4.88 |
| 406.46 | 4.88 | 4.88 |
| 402.50 | 5.05 | 5.05 |
| 422.92 | 5.39 | 5.39 |
| 406.46 | 4.88 | 4.88 |
| 418.50 | 4.75 | 4.75 |
| 417.51 | 4.77 | 4.77 |
| 425.51 | 3.55 | 3.55 |
| 405.46 | 4.35 | 4.35 |
| 458.56 | 6.35 | 6.35 |
| 460.58 | 6.44 | 6.44 |
| 438.55 | 4.46 | 4.46 |
| 418.50 | 5.27 | 5.27 |
| 432.52 | 4.67 | 4.67 |
| 439.54 | 3.85 | 3.85 |
| 431.54 | 5.08 | 5.08 |
| 433.51 | 5.05 | 5.05 |
| 429.52 | 4.83 | 4.83 |
| 402.50 | 5.05 | 5.05 |
| 320.35 | 3.37 | 3.37 |
| 346.39 | 3.93 | 3.93 |
| 344.37 | 3.38 | 3.38 |
| 402.50 | 5.32 | 5.32 |
| 334.38 | 3.71 | 3.71 |
| 378.43 | 3.78 | 3.78 |
| 378.43 | 3.78 | 3.78 |
| 410.48 | 4.99 | 4.99 |
| 424.50 | 5.29 | 5.29 |
| 478.47 | 6.00 | 6.00 |
| 440.50 | 4.35 | 4.35 |
| 440.50 | 4.35 | 4.35 |
| 454.53 | 5.00 | 5.00 |
| 424.50 | 5.55 | 5.55 |
| 379.42 | 3.17 | 3.17 |
| 411.47 | 4.38 | 4.38 |
| 479.46 | 5.40 | 5.40 |
| 453.55 | 5.58 | 5.58 |
| 454.53 | 4.97 | 4.97 |
| 412.45 | 3.78 | 3.78 |
| 439.52 | 5.12 | 5.12 |
| 440.51 | 4.51 | 4.51 |
| 350.38 | 2.73 | 2.73 |
| 351.37 | 2.13 | 2.13 |
| 425.49 | 4.77 | 4.77 |
| 426.48 | 4.16 | 4.16 |
| 436.52 | 5.52 | 5.52 |
| 437.50 | 4.91 | 4.91 |
| 470.53 | 4.35 | 4.35 |
| 456.50 | 4.05 | 4.05 |
| 424.50 | 5.55 | 5.55 |
| 422.49 | 4.91 | 4.91 |
| 456.52 | 5.90 | 5.90 |
| 442.49 | 5.51 | 5.51 |
| 442.49 | 5.51 | 5.51 |
| 442.49 | 5.51 | 5.51 |
| 360.42 | 4.15 | 4.15 |
| 386.41 | 4.54 | 4.54 |
| 360.42 | 4.32 | 4.32 |
| 364.40 | 3.39 | 3.39 |
| 404.47 | 4.26 | 4.26 |
| 411.47 | 4.38 | 4.38 |
| 380.47 | 4.11 | 4.11 |
| 346.39 | 3.86 | 3.86 |
| 392.46 | 4.17 | 4.17 |
| 358.40 | 4.02 | 4.02 |
| 404.47 | 4.26 | 4.26 |
| 390.44 | 3.92 | 3.92 |
| 404.47 | 4.17 | 4.17 |
| 362.43 | 4.49 | 4.49 |
| 394.50 | 4.50 | 4.50 |
| 378.46 | 4.16 | 4.16 |
| 382.85 | 4.37 | 4.37 |
| 411.47 | 4.38 | 4.38 |
| 378.43 | 3.78 | 3.78 |
| 390.44 | 3.88 | 3.88 |
| 359.39 | 3.61 | 3.61 |
| 362.43 | 4.54 | 4.54 |
| 392.46 | 4.17 | 4.17 |
| 404.47 | 4.26 | 4.26 |
| 392.46 | 4.17 | 4.17 |
| 360.42 | 4.27 | 4.27 |
| 376.46 | 4.74 | 4.74 |
| 360.42 | 4.32 | 4.32 |
| 392.48 | 4.55 | 4.55 |
| 376.46 | 4.88 | 4.88 |
| 348.41 | 4.15 | 4.15 |
| 378.43 | 3.73 | 3.73 |
| 362.43 | 4.49 | 4.49 |
| 424.50 | 5.55 | 5.55 |
| 440.50 | 4.99 | 4.99 |
| 397.44 | 4.34 | 4.34 |
| 428.47 | 5.12 | 5.12 |
| 440.50 | 4.99 | 4.99 |
| 444.92 | 5.64 | 5.64 |
| 411.46 | 4.38 | 4.38 |
| 417.51 | 4.23 | 4.23 |
| 428.47 | 5.12 | 5.12 |
| 405.50 | 4.08 | 4.08 |
| 428.47 | 5.12 | 5.12 |
| 465.44 | 5.36 | 5.36 |
| 374.44 | 4.68 | 4.68 |
| 403.49 | 3.84 | 3.84 |
| 334.38 | 3.76 | 3.76 |
| 444.92 | 5.64 | 5.64 |
| 416.51 | 5.05 | 5.05 |
| 440.50 | 4.99 | 4.99 |
| 396.45 | 4.94 | 4.94 |
| 360.42 | 4.25 | 4.25 |
| 428.47 | 5.64 | 5.64 |
| 378.43 | 3.51 | 3.51 |
| 376.46 | 4.79 | 4.79 |
| 362.43 | 4.54 | 4.54 |
| 422.49 | 5.30 | 5.30 |
| 423.48 | 4.83 | 4.83 |
| 425.49 | 4.72 | 4.72 |
| 419.49 | 3.07 | 3.07 |
| 389.46 | 4.14 | 4.14 |
| 472.93 | 5.57 | 5.57 |
| 419.49 | 3.77 | 3.77 |
| 349.39 | 3.22 | 3.22 |
| 382.42 | 5.26 | 5.26 |
| 412.49 | 5.08 | 5.08 |
| 417.51 | 4.92 | 4.92 |
| 417.51 | 4.92 | 4.92 |
| 417.51 | 4.92 | 4.92 |
| 384.40 | 4.05 | 4.05 |
| 362.43 | 4.54 | 4.54 |
| 417.51 | 4.23 | 4.23 |
| 414.51 | 5.63 | 5.63 |
| 406.44 | 3.51 | 3.51 |
| 463.54 | 5.77 | 5.77 |
| 400.44 | 3.71 | 3.71 |
| 425.49 | 4.69 | 4.69 |
| 416.53 | 5.71 | 5.71 |
| 421.46 | 5.01 | 5.01 |
| 402.41 | 2.38 | 2.38 |
| 404.47 | 4.05 | 4.05 |
| 480.57 | 5.88 | 5.88 |
| 506.48 | 5.93 | 5.93 |
| 478.52 | 4.17 | 4.17 |
| 431.52 | 4.75 | 4.75 |
| 428.50 | 4.32 | 4.32 |
| 388.47 | 5.08 | 5.08 |
| 402.50 | 5.32 | 5.32 |
| 402.50 | 5.32 | 5.32 |
| 361.45 | 5.03 | 5.03 |
| 361.45 | 5.03 | 5.03 |
| 375.47 | 5.42 | 5.42 |
| 375.47 | 5.42 | 5.42 |
| 347.42 | 4.64 | 4.64 |
| 347.42 | 4.64 | 4.64 |
| 347.42 | 4.64 | 4.64 |
| 347.42 | 4.64 | 4.64 |
| 362.43 | 4.43 | 4.43 |
| 362.43 | 4.43 | 4.43 |
| 362.43 | 4.43 | 4.43 |
| 362.43 | 4.43 | 4.43 |
| 430.55 | 5.68 | 5.68 |