Basic Information
ID: ALA3706155
Type: Binding
Description: Radioligand Binding Assay: human or rat P2X7-1321N1 cells were collected and frozen @ −80° C. On the day of the experiment, cell membrane preparations were made according to standard published methods. The total assay volume was 100 μl:10 μl compound (10×)+(b) 40 μl tracer (2.5x)+50 μl membrane (2×). The tracer used for the assay was tritiated A-804598. The compound can be prepared as described in the literature. (Donnelly-Roberts, D. Neuropharmacology 2008, 56 (1), 223-229.) Compounds, tracer and membranes were incubated for 1 hour @ 4° C. The assay was terminated by filtration (GF/B filters pre-soaked with 0.3% PEI) and washed with washing buffer (Tris-HCl 50 mM).
Cell type: P2X7-1321N1
Activity Charts
Bioactivity
Activity Types for Assay ALA3706155
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 417.82 | 5.01 | 5.01 |
| 384.27 | 4.65 | 4.65 |
| 374.23 | 3.37 | 3.37 |
| 387.37 | 3.39 | 3.39 |
| 385.25 | 4.04 | 4.04 |
| 418.81 | 4.41 | 4.41 |
| 386.24 | 3.44 | 3.44 |
| 399.38 | 3.46 | 3.46 |
| 407.78 | 3.74 | 3.74 |
| 421.81 | 3.75 | 3.75 |
| 392.22 | 3.51 | 3.51 |
| 357.78 | 2.86 | 2.86 |
| 375.22 | 2.56 | 2.56 |
| 408.77 | 2.93 | 2.93 |
| 398.29 | 4.96 | 4.96 |
| 421.81 | 4.05 | 4.05 |
| 388.26 | 3.68 | 3.68 |
| 439.80 | 4.18 | 4.18 |
| 406.25 | 3.82 | 3.82 |
| 424.83 | 4.47 | 4.47 |
| 391.28 | 4.11 | 4.11 |
| 489.81 | 5.06 | 5.06 |
| 406.80 | 4.34 | 4.34 |
| 424.83 | 4.47 | 4.47 |
| 438.86 | 4.78 | 4.78 |
| 422.80 | 3.24 | 3.24 |
| 422.80 | 3.24 | 3.24 |
| 406.25 | 3.82 | 3.82 |
| 450.85 | 3.80 | 3.80 |
| 422.80 | 3.32 | 3.32 |
| 435.84 | 4.43 | 4.43 |
| 447.85 | 4.61 | 4.61 |
| 435.84 | 4.43 | 4.43 |
| 475.78 | 4.76 | 4.76 |
| 421.81 | 4.13 | 4.13 |
| 436.83 | 3.71 | 3.71 |
| 453.83 | 4.57 | 4.57 |
| 453.83 | 4.57 | 4.57 |
| 402.29 | 4.07 | 4.07 |
| 402.29 | 4.07 | 4.07 |
| 420.28 | 4.21 | 4.21 |
| 420.28 | 4.21 | 4.21 |
| 449.86 | 4.44 | 4.44 |
| 419.38 | 3.92 | 3.92 |
| 419.38 | 3.92 | 3.92 |
| 415.42 | 4.09 | 4.09 |
| 415.42 | 4.09 | 4.09 |
| 420.28 | 4.21 | 4.21 |
| 420.28 | 4.21 | 4.21 |
| 402.29 | 4.07 | 4.07 |
| 402.29 | 4.07 | 4.07 |
| 402.29 | 4.07 | 4.07 |
| 385.83 | 3.55 | 3.55 |
| 385.83 | 3.55 | 3.55 |
| 385.83 | 3.55 | 3.55 |
| 369.38 | 3.04 | 3.04 |
| 369.38 | 3.04 | 3.04 |
| 367.84 | 3.42 | 3.42 |
| 367.84 | 3.42 | 3.42 |
| 449.86 | 4.69 | 4.69 |
| 449.86 | 4.69 | 4.69 |
| 463.89 | 5.25 | 5.25 |
| 463.89 | 5.25 | 5.25 |
| 434.30 | 4.46 | 4.46 |
| 408.77 | 2.93 | 2.93 |
| 391.33 | 3.22 | 3.22 |
| 421.81 | 4.30 | 4.30 |
| 421.81 | 4.30 | 4.30 |
| 421.81 | 4.30 | 4.30 |
| 439.80 | 4.44 | 4.44 |
| 439.80 | 4.44 | 4.44 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 481.88 | 4.88 | 4.88 |
| 493.92 | 4.55 | 4.55 |
| 421.23 | 4.32 | 4.32 |
| 436.80 | 4.55 | 4.55 |