Basic Information
ID: ALA3706156
Type: Binding
Description: FLIPR Assay: 1321N1 cells expressing the recombinant human, rat or mouse P2X7 channel was cultured in HyQ DME/(HyClone/Dulbecco's Modified Eagle Medium) high glucose supplemented with 10% Fetal Bovine Serum (FBS) and appropriate selection marker. Cells were seeded at a density of 25000 cells/well (96-well clear bottom black walled plates) in 100 ul volume/well. On the day of the experiment, cell plates were washed with assay buffer, containing (in mM): 130 NaCl, 2 KCl, 1 CaCl2, 1 MgCl2, 10 HEPES, 5 glucose; pH 7.40 and 300 mOs. After the wash, cells were loaded with the Calcium-4 dye (Molecular Device) and incubated in the dark for 60 minutes. Test compounds were prepared at 250x the test concentration in neat DMSO. Intermediate 96-well compound plates were prepared by transferring 1.2 uL of the compound into 300 uL of assay buffer. A further 3x dilution occurred when transferring 50 uL/well of the compound plate to 100 uL/well in the cell plate.
Cell type: 1321-N1
Activity Charts
Bioactivity
Activity Types for Assay ALA3706156
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 417.82 | 5.01 | 5.01 |
| 384.27 | 4.65 | 4.65 |
| 372.43 | 2.94 | 2.94 |
| 374.23 | 3.37 | 3.37 |
| 388.26 | 3.38 | 3.38 |
| 374.23 | 3.37 | 3.37 |
| 353.81 | 3.03 | 3.03 |
| 387.37 | 3.39 | 3.39 |
| 385.25 | 4.04 | 4.04 |
| 418.81 | 4.41 | 4.41 |
| 386.24 | 3.44 | 3.44 |
| 399.38 | 3.46 | 3.46 |
| 387.78 | 3.06 | 3.06 |
| 407.78 | 3.74 | 3.74 |
| 421.81 | 3.75 | 3.75 |
| 392.22 | 3.51 | 3.51 |
| 357.78 | 2.86 | 2.86 |
| 374.23 | 3.17 | 3.17 |
| 375.22 | 2.56 | 2.56 |
| 408.77 | 2.93 | 2.93 |
| 398.29 | 4.96 | 4.96 |
| 416.28 | 5.10 | 5.10 |
| 399.28 | 4.23 | 4.23 |
| 431.85 | 5.32 | 5.32 |
| 421.81 | 4.05 | 4.05 |
| 388.26 | 3.68 | 3.68 |
| 439.80 | 4.18 | 4.18 |
| 424.83 | 4.47 | 4.47 |
| 391.28 | 4.11 | 4.11 |
| 489.81 | 5.06 | 5.06 |
| 406.80 | 4.34 | 4.34 |
| 424.83 | 4.47 | 4.47 |
| 438.86 | 4.78 | 4.78 |
| 422.80 | 3.24 | 3.24 |
| 422.80 | 3.24 | 3.24 |
| 406.25 | 3.82 | 3.82 |
| 450.85 | 3.80 | 3.80 |
| 422.80 | 3.32 | 3.32 |
| 435.84 | 4.43 | 4.43 |
| 447.85 | 4.61 | 4.61 |
| 435.84 | 4.43 | 4.43 |
| 435.84 | 4.43 | 4.43 |
| 475.78 | 4.76 | 4.76 |
| 372.43 | 2.94 | 2.94 |
| 421.81 | 4.13 | 4.13 |
| 436.83 | 3.71 | 3.71 |
| 453.83 | 4.57 | 4.57 |
| 453.83 | 4.57 | 4.57 |
| 453.83 | 4.57 | 4.57 |
| 402.29 | 4.07 | 4.07 |
| 402.29 | 4.07 | 4.07 |
| 402.29 | 4.07 | 4.07 |
| 420.28 | 4.21 | 4.21 |
| 420.28 | 4.21 | 4.21 |
| 420.28 | 4.21 | 4.21 |
| 449.86 | 4.44 | 4.44 |
| 419.38 | 3.92 | 3.92 |
| 419.38 | 3.92 | 3.92 |
| 419.38 | 3.92 | 3.92 |
| 415.42 | 4.09 | 4.09 |
| 415.42 | 4.09 | 4.09 |
| 415.42 | 4.09 | 4.09 |
| 420.28 | 4.21 | 4.21 |
| 420.28 | 4.21 | 4.21 |
| 420.28 | 4.21 | 4.21 |
| 402.29 | 4.07 | 4.07 |
| 402.29 | 4.07 | 4.07 |
| 402.29 | 4.07 | 4.07 |
| 385.83 | 3.55 | 3.55 |
| 385.83 | 3.55 | 3.55 |
| 385.83 | 3.55 | 3.55 |
| 385.83 | 3.55 | 3.55 |
| 385.83 | 3.55 | 3.55 |
| 369.38 | 3.04 | 3.04 |
| 369.38 | 3.04 | 3.04 |
| 369.38 | 3.04 | 3.04 |
| 367.84 | 3.42 | 3.42 |
| 367.84 | 3.42 | 3.42 |
| 449.86 | 4.69 | 4.69 |
| 449.86 | 4.69 | 4.69 |
| 449.86 | 4.69 | 4.69 |
| 463.89 | 5.25 | 5.25 |
| 463.89 | 5.25 | 5.25 |
| 463.89 | 5.25 | 5.25 |
| 434.30 | 4.46 | 4.46 |
| 408.77 | 2.93 | 2.93 |
| 408.77 | 2.93 | 2.93 |
| 391.33 | 3.22 | 3.22 |
| 421.81 | 4.30 | 4.30 |
| 421.81 | 4.30 | 4.30 |
| 421.81 | 4.30 | 4.30 |
| 439.80 | 4.44 | 4.44 |
| 439.80 | 4.44 | 4.44 |
| 439.80 | 4.44 | 4.44 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 406.25 | 4.07 | 4.07 |
| 481.88 | 4.88 | 4.88 |
| 493.92 | 4.55 | 4.55 |
| 493.92 | 4.55 | 4.55 |
| 421.23 | 4.32 | 4.32 |
| 436.80 | 4.55 | 4.55 |
| 361.45 | 3.38 | 3.38 |
| 361.45 | 3.38 | 3.38 |
| 365.41 | 3.21 | 3.21 |
| 365.41 | 3.21 | 3.21 |
| 365.41 | 3.21 | 3.21 |