Inhibition Assay: Human recombinant adrenergic β1 receptors expressed in CHO-K1 cells are used in modified Tris-HCl buffer pH 7.4. A 25 aliquot is inc...

ID: ALA3706172

Type: Binding

Description: Inhibition Assay: Human recombinant adrenergic β1 receptors expressed in CHO-K1 cells are used in modified Tris-HCl buffer pH 7.4. A 25 aliquot is incubated with 0.03 nM [125I]Cyanopindolol for 120 minutes at 25° C. Non-specific binding is estimated in the presence of 100 μM S(−)-Propranolol. Receptors are filtered and washed, the filters are then counted to determine [125I]Cyanopindolol specifically bound. Compounds are screened at 10 μM.

Format: BAO_0000219

Organism: Homo sapiens

Cell type: CHO-K1

Target:  Beta-1 adrenergic receptor(ALA213)

Document:  ALA3639212

BindingDB Source:  7107