Basic Information
ID: ALA3706187
Type: Binding
Description: BIOMOL Fluorescent-Based HDAC Assay: The BIOMOL fluorescent-based HDAC activity assay has been applied in In vitro HDACi assay for determination of IC50 values of the compounds of this invention. The assay has been carried out in white 96 well formats according to the BIOMOL's method. In order to optimize the experiment conditions and optinal, the exact concentration range of the Deacetylated Standard (biomol, KI-142) that will vary depending on the flurimeter model, the gain setting and the exact excitation and emission wavelengths used. We recommend diluting some of the standard to a relatively low concentration with Assay Buffer (an appropriate series of Deacetylated Standard dilutions from 20 uM to 0.3125 uM), and Assay Buffer as a time zero to a set of wells on the microtiter plate. Experimental to samples (five dilute groups: 100 uM, 20 uM, 4 uM, 0.8 uM, 0.16 uM) should be compared to a time zero.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706187
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 422.60 | 3.95 | 3.95 |
| 427.61 | 4.68 | 4.68 |
| 379.57 | 4.24 | 4.24 |
| 351.52 | 3.46 | 3.46 |
| 351.47 | 2.60 | 2.60 |
| 339.42 | 2.55 | 2.55 |
| 399.56 | 4.25 | 4.25 |
| 349.50 | 3.24 | 3.24 |
| 323.46 | 2.68 | 2.68 |
| 309.44 | 2.22 | 2.22 |
| 463.63 | 3.75 | 3.75 |
| 377.55 | 4.02 | 4.02 |
| 420.36 | 3.38 | 3.38 |