ID: ALA3706196
Type: Binding
Description: Inhibition Assay: Standard ALDH2 reaction mixtures contained 150 uM formaldehyde, 2.5 mM NAD+, 10 mM MgCl2 and 10 nM recombinant human ALDH2 in 50 mM Hepes buffer, pH 7.4, 0.01% Tween 20 in a final volume of 50 ul using 384-well plates. After 60 min of pre-incubation of compound with ALDH2 and formaldehyde, the reaction was started by adding NAD+ and the reaction mixture was allowed to proceed for 90 minutes. Activity of the enzyme was determined by monitoring NADH formation using Perkin-Elmer Envision Reader with excitation and emission wavelengths set at 340 and 460 nm, respectively.
Format: BAO_0000357
Organism: Homo sapiens
Target: Aldehyde dehydrogenase(ALA1935)
Document: ALA3638424
BindingDB Source: 6845
Bioactivity
Activity Types for Assay ALA3706196
IC50
Parent Molecular Weight | ALogP | Polar Surface Area |
---|---|---|
447.32 | 4.30 | 4.30 |
373.24 | 4.28 | 4.28 |
356.78 | 3.76 | 3.76 |
352.82 | 3.93 | 3.93 |
332.40 | 3.59 | 3.59 |
387.27 | 4.59 | 4.59 |
374.77 | 3.90 | 3.90 |
387.27 | 4.59 | 4.59 |
387.27 | 4.29 | 4.29 |
340.33 | 3.25 | 3.25 |
356.78 | 3.76 | 3.76 |
391.23 | 4.42 | 4.42 |
391.23 | 4.42 | 4.42 |
483.24 | 3.82 | 3.82 |