Inhibition Assay: Standard ALDH2 reaction mixtures contained 150 uM formaldehyde, 2.5 mM NAD+, 10 mM MgCl2 and 10 nM recombinant human ALDH2 in 50 mM ...

Basic Information

ID: ALA3706196

Type: Binding

Description: Inhibition Assay: Standard ALDH2 reaction mixtures contained 150 uM formaldehyde, 2.5 mM NAD+, 10 mM MgCl2 and 10 nM recombinant human ALDH2 in 50 mM Hepes buffer, pH 7.4, 0.01% Tween 20 in a final volume of 50 ul using 384-well plates. After 60 min of pre-incubation of compound with ALDH2 and formaldehyde, the reaction was started by adding NAD+ and the reaction mixture was allowed to proceed for 90 minutes. Activity of the enzyme was determined by monitoring NADH formation using Perkin-Elmer Envision Reader with excitation and emission wavelengths set at 340 and 460 nm, respectively.

Format: BAO_0000357

Organism: Homo sapiens

Target:  Aldehyde dehydrogenase(ALA1935)

Document:  ALA3638424

BindingDB Source:  6845

Activity Charts

Compound Summary

Parent Molecular WeightALogPPolar Surface Area
447.324.304.30
373.244.284.28
356.783.763.76
352.823.933.93
332.403.593.59
387.274.594.59
374.773.903.90
387.274.594.59
387.274.294.29
340.333.253.25
356.783.763.76
391.234.424.42
391.234.424.42
483.243.823.82