Basic Information
ID: ALA3706209
Type: Binding
Description: Inhibition Assay: The prepared substances were tested for TAFIa inhibition using the Actichrome plasma TAFI activity kit from American Diagnostica (Pr. No. 874). This entailed adding 29 μl of assay buffer (20 mM Hepes, 150 mM NaCl, pH 7.4) and 10 μl of TAFIa (American Diagnostica Pr. No. 874TAFIA; 2.5/ml) to 1 μl of 5 mM DMSO solution of the substance and incubating in a 96 half-well microtiter plate at room temperature for 15 minutes. The enzymic reaction was started by adding 10 μl of TAFIa developer (prediluted 1:2 with water). The time course of the reaction was followed at 420 nm in a microtiter plate reader (SpectraMax plus 384; Molecular Devices) for 15 minutes.
Organism: Homo sapiens
Tissue: Plasma
Activity Charts
Bioactivity
Activity Types for Assay ALA3706209
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 314.39 | 2.78 | 2.78 |
| 300.36 | 2.39 | 2.39 |
| 315.38 | 1.20 | 1.20 |
| 391.43 | 2.25 | 2.25 |
| 455.52 | 3.18 | 3.18 |
| 442.48 | 3.90 | 3.90 |
| 537.66 | 4.73 | 4.73 |
| 433.51 | 2.97 | 2.97 |
| 475.59 | 3.53 | 3.53 |
| 523.64 | 4.34 | 4.34 |
| 475.59 | 3.53 | 3.53 |
| 406.36 | 3.22 | 3.22 |
| 412.90 | 4.19 | 4.19 |
| 432.46 | 4.25 | 4.25 |
| 270.29 | 0.90 | 0.90 |
| 342.44 | 3.41 | 3.41 |
| 408.46 | 2.79 | 2.79 |