Basic Information
ID: ALA3706210
Type: Binding
Description: Fluorometric Assay: Compounds were tested for their ability to inhibit histone deacetylase 8 using an in vitro deacetylation assay. In a detailed procedure, 8 μl of HDAC8 enzyme solution (0.125 μg/μl) was transferred to assay plates (CORNING 3676). 1 μl of half-log diluted compounds were added into wells and incubated for 15 min at rt. After that, 8 μl of substrate solution was transferred to the wells and incubated for 30 min at rt. After deacetylation of the substrate by incubation with HDAC8 enzyme, subsequent exposure to a developing reagent produced a fluorophore that was directly proportional to the level of deacetylation. So 4 μl of developer regent (Cayman 10006394) was added into the wells in the assay plates and incubated for 15 min. The fluorescence signal was measured by a FlexStation3 plate reader (excitation wave length 340-360 nm; emission wave length 440-465 nm).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706210
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 300.33 | 2.91 | 2.91 |
| 341.80 | 3.30 | 3.30 |
| 283.33 | 2.17 | 2.17 |
| 284.32 | 1.56 | 1.56 |
| 360.42 | 3.23 | 3.23 |
| 394.86 | 3.89 | 3.89 |
| 394.86 | 3.89 | 3.89 |
| 394.86 | 3.89 | 3.89 |
| 361.41 | 2.63 | 2.63 |
| 375.43 | 2.65 | 2.65 |
| 364.40 | 1.78 | 1.78 |
| 380.85 | 2.29 | 2.29 |
| 362.39 | 2.11 | 2.11 |
| 361.24 | 3.54 | 3.54 |
| 327.34 | 2.68 | 2.68 |
| 328.33 | 2.08 | 2.08 |
| 352.32 | 2.58 | 2.58 |
| 342.40 | 2.27 | 2.27 |
| 374.44 | 3.54 | 3.54 |
| 378.41 | 3.37 | 3.37 |
| 403.49 | 3.30 | 3.30 |
| 392.43 | 3.68 | 3.68 |
| 420.47 | 3.25 | 3.25 |
| 428.41 | 4.25 | 4.25 |
| 429.31 | 4.54 | 4.54 |
| 408.89 | 4.19 | 4.19 |
| 412.85 | 4.02 | 4.02 |
| 452.51 | 5.02 | 5.02 |
| 375.43 | 2.94 | 2.94 |
| 375.43 | 2.94 | 2.94 |
| 391.43 | 2.64 | 2.64 |
| 391.43 | 2.64 | 2.64 |
| 395.85 | 3.28 | 3.28 |
| 395.85 | 3.28 | 3.28 |
| 446.51 | 2.46 | 2.46 |
| 390.44 | 3.67 | 3.67 |
| 410.48 | 4.39 | 4.39 |
| 411.47 | 3.78 | 3.78 |
| 352.37 | 2.86 | 2.86 |
| 337.38 | 1.89 | 1.89 |
| 341.37 | 1.98 | 1.98 |
| 326.42 | 1.38 | 1.38 |
| 380.85 | 2.29 | 2.29 |
| 414.41 | 2.66 | 2.66 |
| 382.39 | 1.92 | 1.92 |
| 406.46 | 1.66 | 1.66 |
| 415.30 | 2.95 | 2.95 |
| 415.30 | 2.95 | 2.95 |
| 347.40 | 1.04 | 1.04 |
| 379.39 | 2.77 | 2.77 |
| 398.84 | 2.43 | 2.43 |
| 398.84 | 2.43 | 2.43 |
| 341.37 | 1.58 | 1.58 |
| 345.79 | 2.23 | 2.23 |
| 345.79 | 2.23 | 2.23 |
| 379.34 | 2.59 | 2.59 |
| 380.23 | 2.88 | 2.88 |
| 387.44 | 3.24 | 3.24 |
| 381.84 | 1.77 | 1.77 |
| 381.84 | 1.77 | 1.77 |
| 365.39 | 1.26 | 1.26 |
| 431.39 | 2.02 | 2.02 |
| 407.45 | 1.14 | 1.14 |
| 395.87 | 1.80 | 1.80 |
| 363.38 | 2.07 | 2.07 |