Basic Information
ID: ALA3706230
Type: Binding
Description: Competitive Binding Assay: Competitive Binding Test of Drug to the Receptor (Rat Heart TSPO) and Radioligand (3H-PK11195): (1) tubes were placed in reaction condition of 30 C.(2) all tubes were added with 20 ug of receptor protein in order;(3) the test tubes were added in order with 20 uL of drugs in certain concentrations;(4) the non-specific binding tubes were added with 20 uL of non-tagged ligand (PK11195), the final concentration of the non-tagged ligand was 0.1 mM, pre-reacted for 30 min;(5) all tubes were added in order with 25 uL 3H-PK11195, the final concentration of the labeled ligand was 1.5 nM;(6) Tris-HCl Buffer pH7.4 was used to complement the reaction volumes of all tubes to reach 200 uL;(7) the reaction was performed under 30 C. reaction condition for 1 h;(8) Then samples were applied to 49-type glass fibre filter, vacuum suction filtered, then washed with 2 ml ice-cold Tris-HCl buffer solution (50 mM Tris-HCl buffer solution, 1 mM EDTA, 5 mM MgCl2, 1 mM PMSF, 0.1% NaN3.
Organism: Rattus norvegicus
Activity Charts
Bioactivity
Activity Types for Assay ALA3706230
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 417.94 | 5.55 | 5.55 |
| 452.39 | 6.21 | 6.21 |
| 453.37 | 5.60 | 5.60 |
| 453.37 | 5.60 | 5.60 |
| 483.40 | 5.23 | 5.23 |
| 397.52 | 5.21 | 5.21 |
| 398.51 | 4.60 | 4.60 |