Basic Information
ID: ALA3706271
Type: Binding
Description: Inhibition Assay: Inhibition of kinase activity of Mnk1 and Mnk2a was assessed with the same assay system, using pre-activated GST-Mnk1 or GST-Mnk2a, respectively. The kinase reaction contains 30 uM substrate peptide, 20 uM ATP, 60 nM ligand and one of either 25 nM pre-activated Mnk1 or 2.5 nM pre-activated Mnk2a. The reaction buffer conditions are 16 mM HEPES/KOH pH 7.4, 8 mM MgCl2, 0.4 mM DTT, 0.08% (w/v) bovine serum albumin (BSA, Sigma, Germany, cat. no. A3059), 0.008% (w/v) Pluronic F127 (Sigma, Germany, cat. no. P2443), 3% (v/v) DMSO (Applichem, Germany, cat. no. A3006). The kinase reaction is at 30 C. for 40 min. The kinase reaction is terminated by addition of 0.67 reaction volumes of 1 uM antibody in 20 mM HEPES/KOH pH 7.4, 50 mM ethylenediaminetetraacetic acid, disodium salt (EDTA, Sigma, Germany, cat. no. E5134), 0.5 mM DTT, 0.05% (w/v) polyoxyethylene-sorbitan monolaureate (Tween 20, Sigma, Germany, cat. no. P7949).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706271
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 476.87 | 5.87 | 5.87 |
| 375.43 | 4.46 | 4.46 |
| 445.56 | 4.35 | 4.35 |
| 476.53 | 4.87 | 4.87 |
| 489.57 | 4.53 | 4.53 |
| 560.70 | 4.86 | 4.86 |
| 519.60 | 3.90 | 3.90 |
| 390.44 | 3.40 | 3.40 |
| 460.58 | 3.29 | 3.29 |
| 419.48 | 2.33 | 2.33 |
| 389.46 | 2.97 | 2.97 |
| 468.53 | 2.99 | 2.99 |
| 496.59 | 3.76 | 3.76 |
| 538.67 | 2.88 | 2.88 |
| 432.48 | 3.57 | 3.57 |
| 460.53 | 4.21 | 4.21 |
| 418.49 | 4.00 | 4.00 |
| 475.55 | 4.27 | 4.27 |
| 475.55 | 4.27 | 4.27 |
| 375.43 | 2.71 | 2.71 |
| 376.41 | 3.31 | 3.31 |
| 471.48 | 4.29 | 4.29 |
| 376.41 | 3.01 | 3.01 |
| 404.47 | 3.79 | 3.79 |
| 373.44 | 2.65 | 2.65 |
| 404.47 | 3.79 | 3.79 |
| 482.56 | 3.38 | 3.38 |
| 446.50 | 3.96 | 3.96 |
| 474.56 | 4.60 | 4.60 |
| 503.60 | 4.93 | 4.93 |
| 432.52 | 4.39 | 4.39 |
| 387.47 | 3.04 | 3.04 |
| 373.44 | 2.95 | 2.95 |
| 457.60 | 2.94 | 2.94 |
| 372.45 | 2.35 | 2.35 |
| 405.46 | 2.33 | 2.33 |
| 447.49 | 2.51 | 2.51 |
| 483.55 | 1.92 | 1.92 |
| 433.51 | 4.60 | 4.60 |
| 419.48 | 4.51 | 4.51 |
| 503.64 | 4.49 | 4.49 |
| 398.37 | 4.17 | 4.17 |
| 397.38 | 3.57 | 3.57 |
| 494.54 | 4.29 | 4.29 |
| 482.53 | 4.15 | 4.15 |
| 411.41 | 3.83 | 3.83 |
| 492.53 | 3.76 | 3.76 |
| 441.44 | 3.19 | 3.19 |
| 448.52 | 3.80 | 3.80 |
| 411.41 | 4.36 | 4.36 |
| 397.38 | 4.27 | 4.27 |
| 396.39 | 3.67 | 3.67 |
| 410.42 | 3.93 | 3.93 |
| 551.59 | 4.96 | 4.96 |
| 481.54 | 4.25 | 4.25 |
| 467.52 | 3.86 | 3.86 |
| 440.45 | 3.29 | 3.29 |
| 491.54 | 3.86 | 3.86 |
| 507.58 | 4.78 | 4.78 |
| 474.49 | 3.26 | 3.26 |
| 451.47 | 3.40 | 3.40 |
| 501.63 | 4.37 | 4.37 |
| 362.39 | 3.57 | 3.57 |
| 405.46 | 2.60 | 2.60 |
| 446.55 | 3.56 | 3.56 |
| 373.44 | 2.65 | 2.65 |
| 417.49 | 2.27 | 2.27 |
| 458.59 | 3.23 | 3.23 |
| 345.38 | 1.61 | 1.61 |
| 430.53 | 2.19 | 2.19 |
| 366.35 | 3.48 | 3.48 |
| 365.37 | 2.88 | 2.88 |
| 409.42 | 2.50 | 2.50 |
| 450.52 | 3.46 | 3.46 |
| 432.48 | 3.57 | 3.57 |
| 418.45 | 3.48 | 3.48 |
| 417.47 | 2.88 | 2.88 |
| 431.49 | 3.15 | 3.15 |
| 502.62 | 3.47 | 3.47 |
| 475.55 | 3.11 | 3.11 |
| 409.47 | 4.00 | 4.00 |
| 394.46 | 3.31 | 3.31 |
| 406.41 | 3.96 | 3.96 |
| 391.40 | 3.27 | 3.27 |
| 358.42 | 3.71 | 3.71 |
| 428.56 | 3.61 | 3.61 |
| 454.60 | 4.14 | 4.14 |
| 415.52 | 3.43 | 3.43 |
| 371.47 | 3.68 | 3.68 |
| 470.60 | 3.38 | 3.38 |
| 442.55 | 2.64 | 2.64 |
| 398.49 | 2.75 | 2.75 |
| 414.49 | 2.40 | 2.40 |
| 424.49 | 3.84 | 3.84 |
| 454.60 | 4.14 | 4.14 |
| 440.57 | 3.75 | 3.75 |
| 401.49 | 3.04 | 3.04 |
| 387.47 | 2.65 | 2.65 |
| 401.49 | 3.04 | 3.04 |
| 442.55 | 2.64 | 2.64 |
| 437.53 | 3.59 | 3.59 |
| 438.56 | 3.22 | 3.22 |
| 441.56 | 3.96 | 3.96 |
| 382.45 | 3.18 | 3.18 |
| 410.50 | 2.62 | 2.62 |
| 461.52 | 2.51 | 2.51 |
| 416.51 | 1.98 | 1.98 |
| 494.60 | 1.57 | 1.57 |
| 451.53 | 2.54 | 2.54 |
| 450.55 | 1.94 | 1.94 |
| 535.70 | 2.53 | 2.53 |
| 418.43 | 4.09 | 4.09 |
| 514.60 | 4.52 | 4.52 |
| 488.56 | 3.98 | 3.98 |
| 500.58 | 4.13 | 4.13 |
| 447.47 | 3.02 | 3.02 |
| 498.56 | 3.60 | 3.60 |
| 442.45 | 3.55 | 3.55 |
| 371.40 | 3.27 | 3.27 |
| 405.41 | 3.52 | 3.52 |
| 461.52 | 2.90 | 2.90 |
| 490.56 | 1.84 | 1.84 |
| 447.49 | 2.51 | 2.51 |
| 476.53 | 1.45 | 1.45 |
| 404.47 | 3.52 | 3.52 |
| 456.55 | 3.85 | 3.85 |
| 412.45 | 4.65 | 4.65 |
| 383.35 | 4.22 | 4.22 |
| 409.39 | 4.61 | 4.61 |
| 409.41 | 3.94 | 3.94 |
| 379.39 | 4.32 | 4.32 |
| 363.37 | 2.95 | 2.95 |
| 391.42 | 3.99 | 3.99 |
| 415.37 | 4.91 | 4.91 |
| 379.39 | 4.32 | 4.32 |
| 489.59 | 4.69 | 4.69 |
| 489.59 | 4.83 | 4.83 |
| 475.57 | 4.44 | 4.44 |
| 455.52 | 3.68 | 3.68 |
| 496.61 | 4.64 | 4.64 |
| 485.51 | 4.51 | 4.51 |
| 382.37 | 3.63 | 3.63 |
| 426.42 | 3.25 | 3.25 |
| 467.52 | 4.21 | 4.21 |
| 452.46 | 3.64 | 3.64 |
| 408.41 | 4.02 | 4.02 |
| 493.56 | 4.60 | 4.60 |
| 452.48 | 2.97 | 2.97 |
| 493.58 | 3.93 | 3.93 |
| 463.55 | 4.30 | 4.30 |
| 422.46 | 3.34 | 3.34 |
| 378.40 | 3.72 | 3.72 |
| 436.47 | 1.34 | 1.34 |
| 392.41 | 1.71 | 1.71 |
| 477.56 | 2.30 | 2.30 |
| 362.39 | 2.35 | 2.35 |
| 406.44 | 1.98 | 1.98 |
| 447.54 | 2.94 | 2.94 |
| 475.59 | 3.98 | 3.98 |
| 434.49 | 3.02 | 3.02 |
| 390.44 | 3.40 | 3.40 |
| 499.53 | 4.90 | 4.90 |
| 463.55 | 4.30 | 4.30 |
| 525.57 | 5.43 | 5.43 |
| 539.60 | 5.82 | 5.82 |