Basic Information
ID: ALA3706275
Type: Binding
Description: Inhibition Assay: After the present compound dissolved in DMSO was added to become 0.5% DMSO to the reaction buffer consisting of 20 mM tris hydrochloric acid (pH7.4), bovine serum albumin (0.5%), calcium chloride (4 mM), sodium chloride (150 mM) and human HDL (2 mg/ml), the EL enzyme was added (total volume was 20 ul).After 4-hour reaction at 37 C., non-esterified fatty acid (NEFA) generated from HDL by EL was measured with a commercially available assay kit and the amount of NEFA was used as an index of enzyme activity. Considering the enzyme activity without the inhibitor as a control value, the inhibition rate of each concentration of the present compound was calculated, and 50% inhibitory concentration (IC50 value) was calculated from an inhibition curve.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706275
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 437.55 | 3.47 | 3.47 |
| 429.55 | 4.52 | 4.52 |
| 356.45 | 2.63 | 2.63 |
| 470.47 | 5.54 | 5.54 |
| 409.49 | 3.98 | 3.98 |
| 390.47 | 2.79 | 2.79 |
| 383.47 | 2.12 | 2.12 |
| 394.46 | 2.27 | 2.27 |
| 363.44 | 2.37 | 2.37 |
| 448.43 | 3.16 | 3.16 |
| 402.48 | 4.52 | 4.52 |
| 364.43 | 2.26 | 2.26 |