Basic Information
ID: ALA3706282
Type: Binding
Description: Flashplate Assay: The kinase assay is performed as 384-well flashplate assay (for example for Topcount measurement).0.6 nM TANK binding kinase (TBK1), 800 nM biotinylated MELK-derived peptide (Biotin-Ah-Ah-AKPKGNKDYHLQTCCGSLAYRRR) and 10 uM ATP (spiked with 0.25 uCi of 33P-ATP/well) are incubated at 30 C. for 120 min in a total volume of 50 ul (10 mM MOPS, 10 mM Mg acetate, 0.1 mM EGTA, 1 mM DTT, 0.02% of Brij35, 0.1% of BSA, pH 7.5) with or without test compound. The reaction is stopped with 25 ul of 200 mM EDTA. After 30 min at room temperature, the liquid is removed, and each well is washed three times with 100 ul of 0.9% sodium chloride solution. Non-specific reaction is measured in the presence of 100 nM staurosporine. The radioactivity is measured in a Topcount (PerkinElmer).
Format: BAO_0000357
Target: Serine/threonine-protein kinase TBK1(ALA5408)
Document: ALA3638777
BindingDB Source: 6658
Activity Charts
Bioactivity
Activity Types for Assay ALA3706282
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 445.53 | 3.44 | 3.44 |
| 416.44 | 4.01 | 4.01 |
| 456.55 | 4.18 | 4.18 |
| 446.51 | 3.14 | 3.14 |
| 404.43 | 2.41 | 2.41 |
| 390.40 | 2.40 | 2.40 |
| 403.44 | 3.20 | 3.20 |
| 456.55 | 4.18 | 4.18 |
| 456.55 | 4.18 | 4.18 |
| 460.54 | 3.99 | 3.99 |
| 455.56 | 4.78 | 4.78 |
| 406.45 | 2.51 | 2.51 |
| 484.56 | 4.10 | 4.10 |
| 401.47 | 3.78 | 3.78 |
| 469.59 | 5.13 | 5.13 |
| 405.46 | 3.11 | 3.11 |
| 377.40 | 3.61 | 3.61 |
| 429.52 | 4.38 | 4.38 |
| 457.53 | 4.15 | 4.15 |
| 459.55 | 3.61 | 3.61 |
| 470.58 | 4.07 | 4.07 |
| 458.52 | 3.55 | 3.55 |
| 454.53 | 5.47 | 5.47 |
| 419.51 | 4.65 | 4.65 |
| 483.57 | 4.15 | 4.15 |
| 456.55 | 4.18 | 4.18 |
| 458.52 | 3.55 | 3.55 |
| 455.56 | 4.78 | 4.78 |
| 455.52 | 4.86 | 4.86 |
| 377.40 | 3.61 | 3.61 |
| 390.45 | 3.13 | 3.13 |
| 430.51 | 3.76 | 3.76 |
| 456.55 | 4.18 | 4.18 |
| 444.54 | 4.61 | 4.61 |
| 457.53 | 4.61 | 4.61 |
| 403.44 | 3.20 | 3.20 |
| 457.53 | 4.61 | 4.61 |
| 460.54 | 3.99 | 3.99 |
| 403.44 | 3.20 | 3.20 |
| 404.47 | 3.78 | 3.78 |
| 471.56 | 4.15 | 4.15 |
| 331.38 | 3.88 | 3.88 |
| 389.46 | 4.00 | 4.00 |
| 372.43 | 4.04 | 4.04 |
| 414.51 | 4.95 | 4.95 |
| 427.51 | 4.38 | 4.38 |
| 345.41 | 4.27 | 4.27 |
| 403.49 | 4.39 | 4.39 |
| 386.46 | 4.43 | 4.43 |
| 428.54 | 5.34 | 5.34 |
| 439.56 | 5.33 | 5.33 |
| 474.57 | 4.52 | 4.52 |
| 457.53 | 4.57 | 4.57 |
| 499.62 | 4.80 | 4.80 |
| 512.61 | 4.91 | 4.91 |
| 458.57 | 4.38 | 4.38 |
| 376.42 | 4.22 | 4.22 |
| 375.43 | 3.66 | 3.66 |
| 457.53 | 4.15 | 4.15 |
| 438.49 | 4.71 | 4.71 |
| 417.51 | 4.94 | 4.94 |
| 397.44 | 4.19 | 4.19 |
| 401.47 | 4.52 | 4.52 |
| 440.43 | 5.34 | 5.34 |
| 373.42 | 3.71 | 3.71 |
| 402.45 | 3.81 | 3.81 |
| 444.54 | 4.04 | 4.04 |
| 402.45 | 3.81 | 3.81 |
| 454.49 | 4.77 | 4.77 |
| 425.44 | 4.38 | 4.38 |
| 472.59 | 4.51 | 4.51 |
| 474.57 | 3.45 | 3.45 |
| 433.51 | 4.15 | 4.15 |
| 440.51 | 4.28 | 4.28 |
| 416.49 | 3.26 | 3.26 |
| 445.52 | 3.75 | 3.75 |
| 445.52 | 4.15 | 4.15 |
| 430.51 | 3.65 | 3.65 |
| 530.63 | 3.74 | 3.74 |
| 529.65 | 3.71 | 3.71 |
| 418.50 | 5.21 | 5.21 |
| 376.42 | 3.05 | 3.05 |
| 362.39 | 3.04 | 3.04 |
| 420.47 | 3.16 | 3.16 |
| 415.45 | 3.42 | 3.42 |
| 455.52 | 4.01 | 4.01 |
| 475.55 | 2.85 | 2.85 |
| 431.50 | 3.05 | 3.05 |
| 446.51 | 3.55 | 3.55 |
| 455.47 | 4.16 | 4.16 |
| 456.51 | 3.41 | 3.41 |
| 426.43 | 3.78 | 3.78 |
| 473.58 | 3.90 | 3.90 |