Basic Information
ID: ALA3706283
Type: Binding
Description: Radioligand Binding Assay: Human Embryonic Kidney 293 cells (HEK293) stably expressing rat orexin 1 receptor(Genebank accession number NM_001525) or Chinese ovary cells (CHO) stably expressing human orexin 1 receptor (Genebank accession number NM_001526) were grown to confluency in DMEM (Hyclone, cat # SH30022), 10% FBS, IX Pen/Strep, IX sodium pyruvate, 10 mM HEPES, 600 ug/mL G418 and DMEM/F12 (Gibco, Cat #1 1039), 10%FBS, IX Pen/Strep, 600 ug/mL G418 media, respectively on 150 cm2 tissue culture plates, washed with 5 mM EDTA in PBS (HyClone Dulbecco's Phoshpate Buffered Saline IX with Calcium and Magnesium, Cat # SH30264.01, hereafter referred to simply as PBS) and scraped into 50 ml tubes. After centrifugation (2K xG, 5 min at 4 C), the supernatant was aspirated and the pellets frozen and stored at -80C. Cells were resuspended in PBS in the presence of 1 tablet of protease inhibitor cocktail (Roche, Cat. #1 1836145001) per 50 mL. Each cell pellet from a 15 cm plate was resuspended in 10 mL.
Organism: Rattus norvegicus
Cell type: ovary
Activity Charts
Bioactivity
Activity Types for Assay ALA3706283
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 464.88 | 4.44 | 4.44 |
| 445.45 | 4.06 | 4.06 |
| 420.49 | 4.40 | 4.40 |
| 416.52 | 4.65 | 4.65 |
| 411.40 | 4.35 | 4.35 |
| 407.44 | 4.60 | 4.60 |
| 430.43 | 3.79 | 3.79 |
| 441.33 | 3.53 | 3.53 |
| 422.30 | 4.10 | 4.10 |
| 418.33 | 4.35 | 4.35 |
| 436.90 | 4.17 | 4.17 |
| 413.90 | 4.99 | 4.99 |
| 417.87 | 4.73 | 4.73 |
| 413.49 | 3.32 | 3.32 |
| 394.45 | 3.88 | 3.88 |
| 390.49 | 4.13 | 4.13 |
| 439.52 | 3.83 | 3.83 |
| 420.49 | 4.40 | 4.40 |
| 416.52 | 4.65 | 4.65 |
| 446.44 | 2.88 | 2.88 |
| 445.45 | 3.49 | 3.49 |
| 446.44 | 2.88 | 2.88 |
| 417.47 | 3.22 | 3.22 |
| 417.47 | 3.22 | 3.22 |
| 417.47 | 3.22 | 3.22 |
| 411.89 | 4.20 | 4.20 |
| 457.46 | 3.76 | 3.76 |
| 445.45 | 3.49 | 3.49 |
| 456.34 | 4.31 | 4.31 |
| 428.50 | 4.09 | 4.09 |
| 461.45 | 3.19 | 3.19 |
| 446.43 | 3.96 | 3.96 |
| 457.46 | 3.76 | 3.76 |
| 412.88 | 3.59 | 3.59 |
| 417.47 | 4.29 | 4.29 |
| 461.45 | 3.19 | 3.19 |
| 449.41 | 3.32 | 3.32 |
| 411.90 | 3.12 | 3.12 |
| 456.35 | 3.23 | 3.23 |
| 445.45 | 3.49 | 3.49 |
| 461.45 | 3.19 | 3.19 |
| 446.44 | 2.88 | 2.88 |
| 460.46 | 3.79 | 3.79 |
| 445.45 | 3.49 | 3.49 |
| 410.91 | 3.73 | 3.73 |
| 447.42 | 2.85 | 2.85 |
| 446.43 | 3.46 | 3.46 |
| 432.41 | 3.15 | 3.15 |
| 447.42 | 2.85 | 2.85 |
| 416.53 | 3.96 | 3.96 |
| 430.43 | 3.79 | 3.79 |
| 444.46 | 4.09 | 4.09 |
| 444.46 | 4.09 | 4.09 |
| 422.45 | 4.31 | 4.31 |
| 445.45 | 3.49 | 3.49 |
| 460.46 | 3.79 | 3.79 |
| 444.46 | 4.09 | 4.09 |
| 431.42 | 3.18 | 3.18 |
| 445.45 | 4.56 | 4.56 |
| 456.47 | 4.37 | 4.37 |
| 448.42 | 3.93 | 3.93 |
| 445.45 | 3.49 | 3.49 |
| 456.47 | 4.37 | 4.37 |
| 411.40 | 4.35 | 4.35 |
| 407.44 | 4.60 | 4.60 |
| 397.87 | 2.82 | 2.82 |
| 377.45 | 2.47 | 2.47 |
| 396.88 | 3.42 | 3.42 |
| 431.42 | 3.18 | 3.18 |
| 380.43 | 2.91 | 2.91 |
| 431.42 | 3.18 | 3.18 |
| 362.44 | 2.77 | 2.77 |
| 376.46 | 3.08 | 3.08 |
| 431.42 | 3.18 | 3.18 |
| 413.49 | 3.32 | 3.32 |
| 381.42 | 2.30 | 2.30 |
| 402.46 | 3.51 | 3.51 |
| 441.33 | 3.53 | 3.53 |
| 422.30 | 4.10 | 4.10 |
| 418.33 | 4.35 | 4.35 |
| 436.90 | 4.17 | 4.17 |
| 413.90 | 4.99 | 4.99 |
| 417.87 | 4.73 | 4.73 |
| 413.49 | 3.32 | 3.32 |
| 390.49 | 4.13 | 4.13 |
| 394.45 | 3.88 | 3.88 |
| 439.52 | 3.83 | 3.83 |
| 446.44 | 2.88 | 2.88 |