Basic Information
ID: ALA3706302
Type: Binding
Description: ADP Glo Assay: The ATPase assay is performed according the following protocol: Purified enzyme (20 nM p97), substrate (20 uM ATP) and a dose titration of compounds are mixed in buffer (50 mM TRIS pH 7.5, 20 mM MgCl2, 0.02% TX-100, 1 mM DTT, 0.2% (v/v) glycerol) and incubated at 37 C. for 15 minutes. The reaction is terminated and the level of product generated is measured using the ADP Glo Assay Kit (Promega, Madison Wis.).
Activity Charts
Bioactivity
Activity Types for Assay ALA3706302
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 373.46 | 4.36 | 4.36 |
| 386.50 | 5.49 | 5.49 |
| 355.44 | 4.84 | 4.84 |
| 369.47 | 5.04 | 5.04 |
| 335.45 | 4.84 | 4.84 |
| 398.47 | 4.78 | 4.78 |
| 414.44 | 4.31 | 4.31 |
| 395.47 | 4.90 | 4.90 |
| 381.44 | 4.59 | 4.59 |
| 411.47 | 4.60 | 4.60 |
| 357.42 | 4.39 | 4.39 |
| 403.47 | 3.63 | 3.63 |
| 425.50 | 3.50 | 3.50 |
| 440.51 | 4.19 | 4.19 |
| 439.48 | 3.03 | 3.03 |
| 410.48 | 4.31 | 4.31 |
| 393.49 | 4.95 | 4.95 |
| 423.52 | 4.22 | 4.22 |
| 408.58 | 5.62 | 5.62 |
| 388.46 | 4.05 | 4.05 |
| 356.39 | 2.89 | 2.89 |
| 356.43 | 3.50 | 3.50 |
| 374.45 | 3.46 | 3.46 |
| 346.39 | 3.72 | 3.72 |
| 410.48 | 4.63 | 4.63 |
| 424.51 | 4.66 | 4.66 |
| 438.49 | 4.55 | 4.55 |
| 395.47 | 4.98 | 4.98 |
| 396.49 | 5.33 | 5.33 |
| 399.45 | 4.47 | 4.47 |
| 396.45 | 4.47 | 4.47 |
| 386.42 | 3.76 | 3.76 |
| 386.42 | 3.76 | 3.76 |
| 397.44 | 3.88 | 3.88 |
| 411.47 | 3.89 | 3.89 |
| 416.51 | 4.11 | 4.11 |
| 394.48 | 5.50 | 5.50 |
| 369.47 | 5.40 | 5.40 |
| 382.47 | 4.44 | 4.44 |
| 382.47 | 4.81 | 4.81 |
| 428.47 | 5.21 | 5.21 |
| 386.46 | 3.08 | 3.08 |
| 369.47 | 3.99 | 3.99 |
| 369.47 | 3.99 | 3.99 |
| 400.44 | 2.72 | 2.72 |
| 355.45 | 3.94 | 3.94 |
| 396.49 | 4.79 | 4.79 |
| 408.51 | 5.76 | 5.76 |
| 410.48 | 5.20 | 5.20 |
| 409.49 | 4.65 | 4.65 |
| 380.45 | 5.19 | 5.19 |
| 394.48 | 5.50 | 5.50 |
| 395.46 | 5.97 | 5.97 |
| 407.48 | 4.59 | 4.59 |
| 356.43 | 4.37 | 4.37 |
| 389.46 | 5.37 | 5.37 |
| 469.59 | 5.63 | 5.63 |
| 397.48 | 4.20 | 4.20 |
| 411.51 | 4.25 | 4.25 |
| 410.52 | 4.54 | 4.54 |
| 499.57 | 4.97 | 4.97 |
| 368.48 | 5.22 | 5.22 |
| 382.51 | 5.47 | 5.47 |
| 383.50 | 4.37 | 4.37 |
| 397.53 | 4.93 | 4.93 |
| 455.52 | 4.33 | 4.33 |
| 455.52 | 4.61 | 4.61 |
| 451.53 | 4.83 | 4.83 |
| 463.54 | 5.00 | 5.00 |
| 409.49 | 5.15 | 5.15 |
| 384.48 | 4.92 | 4.92 |
| 384.48 | 4.40 | 4.40 |
| 398.51 | 5.06 | 5.06 |
| 411.55 | 4.97 | 4.97 |
| 425.54 | 4.55 | 4.55 |
| 426.52 | 4.08 | 4.08 |
| 441.54 | 4.77 | 4.77 |
| 461.59 | 3.96 | 3.96 |
| 425.54 | 4.19 | 4.19 |
| 412.54 | 3.87 | 3.87 |
| 442.52 | 3.62 | 3.62 |
| 426.52 | 3.53 | 3.53 |
| 462.58 | 2.81 | 2.81 |
| 441.58 | 3.07 | 3.07 |
| 455.57 | 2.60 | 2.60 |
| 431.50 | 5.73 | 5.73 |
| 474.52 | 4.59 | 4.59 |
| 484.58 | 5.21 | 5.21 |