Basic Information
ID: ALA3706310
Type: Binding
Description: LANCE ULight TR-FRET Kinase Assay: CDK9 enzyme activities were measured using LANCE ULight TR-FRET kinase assay reagents (PerkinElmer, Waltham, Mass.). Compounds were directly added in 100% DMSO to white low volume assay plates (Perkin Elmer Proxiplate 6008289) using a Labcyte Echo acoustic dispenser. Assay reagents in serine/threonine kinase assay buffer containing 20 mM HEPES, 10 mM MgCl2, 100 mM Na3VO4, and 0.0075% Triton X-100. were added for final reaction mixture concentrations of 1000 uM ATP, 100 nM U-light MBP peptide (Perkin Elmer TRF0109M) and reaction initiated with 4 nM CDK9/Cyclin T1 (Carna Biosciences 04-110). The kinase reaction was carried out for 30 minutes before addition of stopping buffer to a final of 20 mM EDTA and 0.5 nM of LANCE Ultra Europium anti-phospho-MBP antibody (PerkinElmer TRF0201M) in LANCE detection buffer (PerkinElmer CR97-100). The reaction was equilibrated for 1 hour and the signal read in the Perkin Elmer Envision in TR-FRET mode.
Format: BAO_0000357
Organism: Homo sapiens
Target: Cyclin-dependent kinase 9(ALA3116)
Document: ALA3639207
BindingDB Source: 7031
Activity Charts
Bioactivity
Activity Types for Assay ALA3706310
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 325.39 | 3.84 | 3.84 |
| 325.39 | 3.84 | 3.84 |
| 325.39 | 3.84 | 3.84 |
| 325.39 | 3.84 | 3.84 |
| 323.40 | 2.68 | 2.68 |
| 375.48 | 3.06 | 3.06 |
| 347.46 | 4.58 | 4.58 |
| 339.41 | 4.19 | 4.19 |
| 339.41 | 4.19 | 4.19 |
| 367.42 | 4.10 | 4.10 |
| 417.94 | 5.36 | 5.36 |
| 431.97 | 5.70 | 5.70 |
| 397.53 | 5.05 | 5.05 |
| 413.53 | 4.71 | 4.71 |
| 524.09 | 5.81 | 5.81 |
| 475.98 | 5.84 | 5.84 |
| 325.39 | 3.84 | 3.84 |
| 325.39 | 3.84 | 3.84 |
| 341.84 | 4.36 | 4.36 |
| 308.39 | 3.10 | 3.10 |
| 323.40 | 2.68 | 2.68 |
| 343.38 | 3.98 | 3.98 |
| 341.84 | 4.36 | 4.36 |
| 321.42 | 4.01 | 4.01 |
| 341.84 | 4.36 | 4.36 |
| 326.38 | 3.24 | 3.24 |
| 307.40 | 3.71 | 3.71 |
| 339.41 | 4.19 | 4.19 |
| 339.41 | 4.19 | 4.19 |
| 335.45 | 4.36 | 4.36 |
| 322.41 | 3.44 | 3.44 |
| 417.94 | 5.36 | 5.36 |
| 435.93 | 5.50 | 5.50 |
| 418.93 | 4.75 | 4.75 |
| 452.39 | 6.01 | 6.01 |
| 453.92 | 5.64 | 5.64 |
| 436.92 | 4.89 | 4.89 |
| 435.93 | 5.50 | 5.50 |
| 453.92 | 5.64 | 5.64 |
| 418.93 | 4.75 | 4.75 |
| 424.98 | 4.16 | 4.16 |
| 424.98 | 4.16 | 4.16 |
| 388.90 | 5.41 | 5.41 |
| 431.93 | 5.00 | 5.00 |
| 431.97 | 5.38 | 5.38 |
| 446.00 | 5.77 | 5.77 |
| 425.96 | 4.58 | 4.58 |
| 425.96 | 4.58 | 4.58 |
| 466.99 | 5.15 | 5.15 |
| 400.50 | 5.45 | 5.45 |
| 417.94 | 5.36 | 5.36 |
| 416.96 | 5.96 | 5.96 |
| 431.97 | 5.70 | 5.70 |
| 431.97 | 5.92 | 5.92 |
| 397.53 | 5.05 | 5.05 |
| 449.96 | 5.84 | 5.84 |
| 418.93 | 5.32 | 5.32 |
| 311.36 | 3.45 | 3.45 |
| 311.36 | 3.45 | 3.45 |
| 311.36 | 3.45 | 3.45 |
| 311.36 | 3.45 | 3.45 |
| 325.39 | 3.80 | 3.80 |
| 403.92 | 4.97 | 4.97 |
| 411.94 | 4.19 | 4.19 |
| 327.36 | 3.04 | 3.04 |
| 327.36 | 3.04 | 3.04 |
| 341.39 | 3.38 | 3.38 |
| 341.39 | 3.38 | 3.38 |
| 419.92 | 4.55 | 4.55 |
| 355.41 | 3.82 | 3.82 |
| 355.41 | 3.82 | 3.82 |
| 371.87 | 4.33 | 4.33 |
| 339.41 | 4.36 | 4.36 |
| 339.41 | 4.36 | 4.36 |
| 339.41 | 4.15 | 4.15 |
| 417.94 | 5.36 | 5.36 |
| 329.81 | 4.49 | 4.49 |
| 330.79 | 3.88 | 3.88 |
| 417.94 | 5.36 | 5.36 |
| 425.96 | 4.58 | 4.58 |
| 431.97 | 5.70 | 5.70 |
| 475.98 | 5.84 | 5.84 |
| 524.09 | 5.81 | 5.81 |
| 343.83 | 5.01 | 5.01 |
| 431.97 | 5.88 | 5.88 |
| 323.37 | 3.75 | 3.75 |
| 391.52 | 3.93 | 3.93 |
| 462.53 | 3.20 | 3.20 |
| 434.52 | 4.09 | 4.09 |
| 434.93 | 3.99 | 3.99 |
| 429.52 | 3.76 | 3.76 |
| 464.54 | 3.45 | 3.45 |
| 402.50 | 3.50 | 3.50 |
| 537.60 | 5.58 | 5.58 |
| 459.53 | 3.96 | 3.96 |
| 438.55 | 3.89 | 3.89 |
| 427.50 | 3.96 | 3.96 |
| 452.51 | 4.23 | 4.23 |
| 425.58 | 5.48 | 5.48 |
| 440.51 | 3.24 | 3.24 |