Basic Information
ID: ALA3706321
Type: Binding
Description: Enzyme Assay: The assays were all performed in a buffer consisting of 20 mM bicine (pH=7.6), 0.5 mM DTT, 0.005% BSG and 0.002% Tween20, prepared on the day of use. Compounds in 100% DMSO (1 uL) were spotted into polypropylene 384-well V-bottom plates (Greiner) using a Platemate 2x3 outfitted with a 384-channel pipet head (Thermo). DMSO (1 uL) was added to columns 11, 12, 23, 24, rows A-H for the maximum signal control, and SAH, a known product and inhibitor of PRC2 (1 uL) was added to columns 11, 12, 23, 24, rows I-P for the minimum signal control, A cocktail (40 uL) containing the wild-type PRC2 enzyme and H3K27me0 peptide or any of the Y641 mutant enzymes and H3K27me2 peptide was added by Multidrop Combi (Thermo). The compounds were allowed to incubate with PRC2 for 30 min at 25 C., then a cocktail (10 uL) containing a mixture of non-radioactive and 3H-SAM was added to initiate the reaction (final volume=51 uL).
Activity Charts
Bioactivity
Activity Types for Assay ALA3706321
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 572.75 | 4.73 | 4.73 |
| 500.69 | 5.34 | 5.34 |
| 542.72 | 5.11 | 5.11 |
| 486.66 | 5.31 | 5.31 |
| 528.70 | 5.08 | 5.08 |
| 470.62 | 5.00 | 5.00 |
| 473.62 | 4.04 | 4.04 |
| 447.58 | 3.87 | 3.87 |
| 562.72 | 3.04 | 3.04 |
| 463.58 | 3.25 | 3.25 |
| 461.61 | 4.40 | 4.40 |
| 557.74 | 3.92 | 3.92 |
| 558.72 | 4.34 | 4.34 |
| 516.69 | 4.57 | 4.57 |
| 556.75 | 5.50 | 5.50 |
| 514.71 | 5.73 | 5.73 |
| 543.71 | 4.50 | 4.50 |
| 546.72 | 3.80 | 3.80 |
| 447.58 | 4.01 | 4.01 |
| 613.80 | 4.61 | 4.61 |
| 613.80 | 4.61 | 4.61 |
| 518.66 | 3.51 | 3.51 |
| 518.66 | 3.51 | 3.51 |
| 558.73 | 3.31 | 3.31 |
| 561.73 | 2.61 | 2.61 |
| 462.60 | 2.82 | 2.82 |
| 515.70 | 4.15 | 4.15 |
| 559.71 | 3.74 | 3.74 |
| 490.60 | 3.40 | 3.40 |
| 517.67 | 3.97 | 3.97 |
| 557.74 | 4.89 | 4.89 |
| 515.70 | 5.12 | 5.12 |
| 501.68 | 4.73 | 4.73 |
| 556.75 | 5.50 | 5.50 |
| 617.79 | 3.31 | 3.31 |
| 617.79 | 3.31 | 3.31 |
| 516.69 | 3.54 | 3.54 |
| 571.77 | 4.44 | 4.44 |
| 571.77 | 4.44 | 4.44 |
| 476.63 | 3.34 | 3.34 |
| 476.63 | 3.34 | 3.34 |
| 529.73 | 4.66 | 4.66 |
| 575.76 | 3.13 | 3.13 |
| 572.75 | 3.83 | 3.83 |
| 572.75 | 3.83 | 3.83 |
| 575.76 | 3.13 | 3.13 |
| 530.72 | 4.06 | 4.06 |
| 530.72 | 4.06 | 4.06 |
| 614.79 | 4.01 | 4.01 |
| 614.79 | 4.01 | 4.01 |
| 586.78 | 5.12 | 5.12 |
| 600.80 | 5.37 | 5.37 |
| 598.79 | 5.12 | 5.12 |
| 600.80 | 5.51 | 5.51 |
| 612.82 | 5.51 | 5.51 |
| 573.74 | 4.13 | 4.13 |
| 530.71 | 4.96 | 4.96 |
| 585.79 | 4.65 | 4.65 |
| 584.76 | 4.88 | 4.88 |
| 584.76 | 4.88 | 4.88 |
| 556.75 | 5.50 | 5.50 |
| 572.75 | 4.47 | 4.47 |
| 572.75 | 4.47 | 4.47 |
| 574.74 | 5.45 | 5.45 |
| 571.77 | 4.31 | 4.31 |
| 574.74 | 5.45 | 5.45 |
| 570.78 | 5.89 | 5.89 |
| 586.78 | 4.86 | 4.86 |
| 586.78 | 4.86 | 4.86 |
| 586.78 | 4.86 | 4.86 |
| 588.77 | 5.84 | 5.84 |
| 588.77 | 5.84 | 5.84 |
| 606.76 | 6.13 | 6.13 |
| 542.72 | 5.11 | 5.11 |
| 558.72 | 4.08 | 4.08 |
| 560.71 | 5.06 | 5.06 |
| 585.79 | 4.70 | 4.70 |
| 599.82 | 5.04 | 5.04 |
| 586.78 | 5.12 | 5.12 |
| 502.66 | 4.36 | 4.36 |
| 516.69 | 4.62 | 4.62 |
| 530.71 | 5.01 | 5.01 |
| 544.74 | 5.40 | 5.40 |
| 556.75 | 5.40 | 5.40 |
| 558.77 | 5.74 | 5.74 |
| 576.74 | 3.35 | 3.35 |
| 576.74 | 3.35 | 3.35 |
| 570.78 | 5.74 | 5.74 |
| 546.71 | 3.98 | 3.98 |
| 560.74 | 4.37 | 4.37 |
| 590.77 | 3.69 | 3.69 |
| 545.73 | 3.95 | 3.95 |
| 559.76 | 4.34 | 4.34 |
| 566.69 | 5.26 | 5.26 |
| 584.68 | 5.55 | 5.55 |
| 584.76 | 4.73 | 4.73 |
| 503.64 | 4.39 | 4.39 |
| 620.82 | 4.48 | 4.48 |
| 588.75 | 4.75 | 4.75 |
| 602.78 | 4.36 | 4.36 |
| 608.73 | 4.98 | 4.98 |
| 518.71 | 4.33 | 4.33 |
| 640.92 | 5.73 | 5.73 |
| 514.71 | 5.60 | 5.60 |
| 528.74 | 5.90 | 5.90 |
| 582.71 | 6.62 | 6.62 |
| 592.81 | 5.00 | 5.00 |
| 516.69 | 4.39 | 4.39 |
| 504.68 | 5.19 | 5.19 |
| 565.76 | 6.14 | 6.14 |
| 531.71 | 3.97 | 3.97 |
| 515.70 | 4.99 | 4.99 |
| 520.74 | 5.66 | 5.66 |
| 529.73 | 5.30 | 5.30 |
| 533.72 | 5.20 | 5.20 |
| 532.73 | 4.64 | 4.64 |
| 518.71 | 4.33 | 4.33 |
| 515.70 | 4.99 | 4.99 |
| 582.75 | 4.78 | 4.78 |
| 530.72 | 4.69 | 4.69 |
| 532.73 | 4.64 | 4.64 |
| 521.73 | 5.05 | 5.05 |
| 520.74 | 5.66 | 5.66 |
| 521.73 | 5.05 | 5.05 |
| 518.71 | 4.33 | 4.33 |
| 613.85 | 4.74 | 4.74 |
| 572.75 | 4.05 | 4.05 |
| 599.82 | 4.40 | 4.40 |
| 558.73 | 3.71 | 3.71 |
| 516.69 | 4.39 | 4.39 |
| 530.72 | 4.69 | 4.69 |
| 600.80 | 5.38 | 5.38 |
| 651.65 | 5.50 | 5.50 |
| 593.17 | 5.08 | 5.08 |
| 590.74 | 4.87 | 4.87 |
| 518.71 | 4.33 | 4.33 |
| 597.80 | 5.44 | 5.44 |
| 615.82 | 4.71 | 4.71 |
| 600.81 | 4.69 | 4.69 |
| 586.78 | 4.35 | 4.35 |
| 586.78 | 4.52 | 4.52 |
| 600.81 | 4.86 | 4.86 |
| 683.94 | 5.33 | 5.33 |
| 683.94 | 5.16 | 5.16 |
| 601.79 | 4.94 | 4.94 |
| 601.79 | 4.77 | 4.77 |
| 600.81 | 4.83 | 4.83 |
| 628.86 | 5.64 | 5.64 |
| 628.86 | 5.47 | 5.47 |