Basic Information
ID: ALA3706339
Type: Binding
Description: Inhibition Assay: A partial protein of only a kinase domain of FLT3 protein was purchased from Carna Biosciences Inc., Japan, and tests were conducted as in Test Example 5. Test Example 5: A partial protein of only a kinase domain of RET protein was purchased from Carna Biosciences Inc., Japan. The phosphorylation activity toward a peptide substrate was investigated using an EZ reader (Caliper). Test compounds were each mixed with a protein solution to give 8 final concentrations from 100 nM to 0.03 nM, followed by addition of a mixed liquid of ATP and substrate peptide (Caliper) and reaction for 30 minutes. The ATP concentration used was 100 µM. A reaction liquid which contained protein but no test compound (in which the solvent DMSO alone was added at 0.8% in place of the test compound) was prepared, followed by reaction in the same manner with or without ATP addition.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706339
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 552.72 | 2.70 | 2.70 |
| 453.59 | 2.35 | 2.35 |
| 536.73 | 2.81 | 2.81 |
| 467.62 | 2.91 | 2.91 |
| 604.72 | 3.83 | 3.83 |
| 550.75 | 3.12 | 3.12 |
| 618.75 | 4.39 | 4.39 |
| 564.78 | 3.51 | 3.51 |
| 495.67 | 3.44 | 3.44 |
| 564.78 | 3.51 | 3.51 |
| 536.73 | 3.00 | 3.00 |
| 557.14 | 3.21 | 3.21 |
| 522.70 | 2.69 | 2.69 |
| 536.73 | 3.25 | 3.25 |
| 457.55 | 2.36 | 2.36 |
| 483.62 | 2.79 | 2.79 |
| 453.59 | 2.79 | 2.79 |
| 536.73 | 3.00 | 3.00 |