Basic Information
ID: ALA3706344
Type: Binding
Description: Fluorometric Assay: All measurements were performed with a BioAssay Reader HTS-7000Plus for microplates (Perkin Elmer) at 30C. QC activity was evaluated fluorometrically using H-Gln-beta NA. The samples consisted of 0.2 mM fluorogenic substrate, 0.25 U pyroglutamyl aminopeptidase (Unizyme, Horsholm, Denmark) in 0.2 M Tris/HCl, pH 8.0 containing 20 mM EDTA and an appropriately diluted aliquot of QC in a final volume of 250 ul. Excitation/emission wavelengths were 320/410 nm. The assay reactions were initiated by addition of glutaminyl cyclase. QC activity was determined from a standard curve of beta -naphthylamine under assay conditions. One unit is defined as the amount of QC catalyzing the formation of 1 umol pGlu-beta NA from H-Gln-beta NA per minute under the described conditions. In a second fluorometric assay, QC was activity determined using H-Gln-AMC as substrate. Reactions were carried out at 30C. utilizing the NOVOStar reader for microplates (BMG labtechnologies).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706344
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 361.40 | 2.38 | 2.38 |
| 391.43 | 2.38 | 2.38 |
| 361.40 | 2.38 | 2.38 |
| 321.43 | 2.56 | 2.56 |
| 345.36 | 2.09 | 2.09 |
| 335.80 | 3.01 | 3.01 |
| 361.40 | 2.38 | 2.38 |
| 391.43 | 2.38 | 2.38 |
| 361.40 | 2.38 | 2.38 |
| 345.36 | 2.09 | 2.09 |
| 351.41 | 3.51 | 3.51 |
| 359.39 | 1.95 | 1.95 |
| 307.40 | 2.18 | 2.18 |
| 335.80 | 3.02 | 3.02 |
| 369.35 | 3.39 | 3.39 |
| 351.41 | 3.52 | 3.52 |
| 359.39 | 2.14 | 2.14 |