Basic Information
ID: ALA3706347
Type: Binding
Description: Inhibition Assay: To test the efficacy of caspase-3 inhibitors at the cellular level, the ability of selected compounds to inhibit the proteolytic cleavage of PARP (poly ADP-ribose polymerase) was evaluated in live Hela cells.Briefly, in this assay Hela cells are seeded in 96 well plates and incubated for 4 hours with staurosporine, a well characterized inducer of apoptosis, alone or together with different concentrations of compound (50, 25, 10 and 3 uM). After formaldehyde-based fixation, the cells are stained with a fluorescein-labeled anti-cleaved PARP antibody (Cell signaling, Cat#: 9547) and counterstained with Hoechst33342 (Invitrogen, Cat#: H3570) to mark all nuclei.
Organism: Homo sapiens
Cell type: HeLa
Activity Charts
Bioactivity
Activity Types for Assay ALA3706347
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 426.49 | 1.46 | 1.46 |
| 555.63 | 3.10 | 3.10 |
| 249.33 | 0.60 | 0.60 |
| 411.52 | 3.60 | 3.60 |
| 311.40 | 2.03 | 2.03 |
| 774.25 | 2.26 | 2.26 |
| 679.22 | 2.66 | 2.66 |
| 742.85 | 1.49 | 1.49 |
| 674.73 | 1.27 | 1.27 |
| 739.80 | 1.69 | 1.69 |
| 714.79 | 1.31 | 1.31 |
| 670.69 | -0.24 | -0.24 |
| 801.88 | 3.12 | 3.12 |
| 817.87 | 3.05 | 3.05 |
| 923.10 | 3.82 | 3.82 |
| 742.85 | 2.09 | 2.09 |
| 736.80 | 2.70 | 2.70 |
| 801.88 | 3.12 | 3.12 |
| 739.80 | 1.69 | 1.69 |
| 704.76 | 0.85 | 0.85 |
| 718.78 | 1.24 | 1.24 |
| 751.82 | 1.97 | 1.97 |
| 727.79 | 1.62 | 1.62 |
| 193.22 | -0.65 | -0.65 |
| 722.82 | 2.82 | 2.82 |
| 702.78 | 1.45 | 1.45 |