Basic Information
ID: ALA3706356
Type: Binding
Description: In Vitro Cell-Free Assay: Test compounds in aqueous solution were added at a volume of 10 microliters, to a reaction mixture comprising 10 microliters Assay Dilution Buffer (ADB; 20 mM MOPS, pH 7.2, 25 mM beta-glycerolphosphate, 5 mM EGTA, 1 mM sodium orthovanadate and 1 mM dithiothreitol), 10 microliters of substrate peptide (RRRDDDSDDD, dissolved in ADB at a concentration of 1 mM), 10 microliters of recombinant human CK2 (25 ng dissolved in ADB; Upstate). Reactions were initiated by the addition of 10 microliters of ATP Solution (90% 75 mM MgCl2, 75 micromolar ATP dissolved in ADB; 10% [gamma-33P]ATP (stock 1 mCi/100 ul; 3000 Ci/mmol (Perkin Elmer) and maintained for 10 minutes at 30 degrees C. The reactions were quenched with 100 microliters of 0.75% phosphoric acid, then transferred to and filtered through a phosphocellulose filter plate (Millipore). After washing each well 5 times with 0.75% phosphoric acid, the plate was dried under vacuum for 5 min.
Format: BAO_0000357
Organism: Homo sapiens
Target: Casein kinase II alpha(ALA3629)
Document: ALA3638475
BindingDB Source: 7003
Activity Charts
Bioactivity
Activity Types for Assay ALA3706356
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 349.78 | 4.88 | 4.88 |
| 363.80 | 4.97 | 4.97 |
| 335.79 | 4.67 | 4.67 |
| 350.77 | 4.27 | 4.27 |
| 314.34 | 4.83 | 4.83 |
| 315.33 | 4.22 | 4.22 |
| 310.36 | 2.45 | 2.45 |
| 329.36 | 4.09 | 4.09 |
| 343.39 | 4.14 | 4.14 |
| 357.41 | 4.53 | 4.53 |
| 345.36 | 4.23 | 4.23 |
| 367.77 | 5.02 | 5.02 |
| 333.32 | 4.36 | 4.36 |
| 349.78 | 4.88 | 4.88 |
| 245.26 | 2.44 | 2.44 |
| 303.34 | 2.30 | 2.30 |
| 303.34 | 2.91 | 2.91 |
| 302.36 | 2.94 | 2.94 |
| 339.35 | 4.21 | 4.21 |
| 340.34 | 4.10 | 4.10 |
| 373.81 | 4.36 | 4.36 |
| 368.76 | 4.41 | 4.41 |
| 352.30 | 3.90 | 3.90 |
| 334.31 | 3.76 | 3.76 |
| 340.34 | 3.60 | 3.60 |
| 384.32 | 4.64 | 4.64 |
| 365.78 | 3.86 | 3.86 |
| 379.81 | 4.31 | 4.31 |
| 393.83 | 4.34 | 4.34 |
| 393.83 | 4.71 | 4.71 |
| 407.86 | 5.09 | 5.09 |
| 405.85 | 4.85 | 4.85 |
| 371.40 | 4.19 | 4.19 |
| 395.42 | 4.18 | 4.18 |
| 439.40 | 5.21 | 5.21 |
| 428.37 | 5.04 | 5.04 |
| 263.26 | 1.86 | 1.86 |
| 240.22 | 1.77 | 1.77 |
| 254.25 | 1.86 | 1.86 |
| 309.37 | 3.06 | 3.06 |
| 277.73 | 3.89 | 3.89 |
| 359.45 | 2.36 | 2.36 |
| 288.33 | 2.55 | 2.55 |
| 315.40 | 3.12 | 3.12 |
| 335.39 | 4.15 | 4.15 |
| 320.37 | 4.89 | 4.89 |
| 335.39 | 4.15 | 4.15 |
| 357.44 | 2.89 | 2.89 |
| 335.39 | 4.15 | 4.15 |
| 350.40 | 4.90 | 4.90 |
| 336.37 | 4.60 | 4.60 |
| 380.43 | 4.91 | 4.91 |
| 219.70 | 4.10 | 4.10 |
| 344.40 | 4.37 | 4.37 |
| 334.40 | 4.92 | 4.92 |
| 338.36 | 5.03 | 5.03 |
| 372.81 | 5.68 | 5.68 |
| 334.40 | 5.20 | 5.20 |
| 350.40 | 4.90 | 4.90 |
| 354.82 | 5.54 | 5.54 |
| 356.35 | 5.17 | 5.17 |
| 284.34 | 3.72 | 3.72 |
| 346.41 | 4.84 | 4.84 |
| 384.84 | 5.55 | 5.55 |
| 336.37 | 4.60 | 4.60 |
| 405.48 | 4.73 | 4.73 |
| 380.43 | 4.91 | 4.91 |
| 269.29 | 1.92 | 1.92 |
| 316.38 | 2.48 | 2.48 |
| 339.36 | 3.71 | 3.71 |
| 334.39 | 1.94 | 1.94 |
| 329.36 | 4.31 | 4.31 |
| 381.79 | 5.11 | 5.11 |
| 359.39 | 4.32 | 4.32 |
| 273.68 | 2.62 | 2.62 |
| 357.35 | 3.85 | 3.85 |
| 391.80 | 4.50 | 4.50 |
| 345.36 | 3.66 | 3.66 |
| 377.83 | 4.79 | 4.79 |
| 361.38 | 4.27 | 4.27 |
| 351.31 | 4.50 | 4.50 |
| 376.44 | 4.43 | 4.43 |
| 408.44 | 3.11 | 3.11 |
| 359.39 | 3.75 | 3.75 |
| 373.42 | 4.44 | 4.44 |
| 389.42 | 3.68 | 3.68 |
| 375.39 | 3.02 | 3.02 |
| 389.42 | 3.41 | 3.41 |
| 414.47 | 3.78 | 3.78 |
| 430.51 | 4.37 | 4.37 |
| 444.50 | 3.36 | 3.36 |
| 458.52 | 3.75 | 3.75 |
| 397.44 | 4.42 | 4.42 |
| 413.44 | 3.66 | 3.66 |
| 438.49 | 3.77 | 3.77 |
| 482.54 | 3.74 | 3.74 |
| 369.38 | 3.64 | 3.64 |
| 417.77 | 5.90 | 5.90 |
| 397.80 | 4.45 | 4.45 |
| 383.77 | 3.99 | 3.99 |