Basic Information
ID: ALA3706359
Type: Binding
Description: Scintillation Proximity Assay (SPA): The kinase assay is based on the LanthaScreen technology. LanthaScreen is the detection of Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) using lanthanide chelates to measure interactions between various binding partners. In a TR-FRET kinase assay, a long-lifetime lanthanide donor species is conjugated to an antibody that specifically binds to a phosphorylated product of a kinase reaction that is labeled with a suitable acceptor fluorophore. This antibody-mediated interaction brings the lanthanide donor and the acceptor into proximity such that resonance energy transfer can take place, resulting in a detectable increase in the FRET signal.The kinase reactions were performed in 384 well microtiter plates in a total reaction volume of 10.05 μL. The assay plates were prepared with 0.05 μL per well of test compound in the appropriate test concentration, as described under preparation of compound dilutions.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3706359
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 359.42 | 3.22 | 3.22 |
| 372.39 | 1.67 | 1.67 |
| 377.36 | 3.21 | 3.21 |
| 460.54 | 1.75 | 1.75 |
| 470.61 | 3.97 | 3.97 |
| 470.61 | 3.58 | 3.58 |
| 488.60 | 4.47 | 4.47 |
| 456.58 | 3.76 | 3.76 |
| 488.60 | 4.11 | 4.11 |
| 470.61 | 4.34 | 4.34 |
| 456.58 | 3.76 | 3.76 |
| 556.70 | 5.64 | 5.64 |
| 456.58 | 3.99 | 3.99 |
| 442.55 | 3.24 | 3.24 |
| 524.70 | 5.28 | 5.28 |
| 542.69 | 5.42 | 5.42 |
| 524.70 | 5.28 | 5.28 |
| 518.65 | 5.16 | 5.16 |
| 457.56 | 4.05 | 4.05 |
| 470.61 | 4.02 | 4.02 |
| 471.59 | 4.44 | 4.44 |
| 474.57 | 3.77 | 3.77 |
| 497.63 | 4.84 | 4.84 |
| 475.55 | 3.93 | 3.93 |
| 443.54 | 4.03 | 4.03 |
| 417.50 | 3.23 | 3.23 |
| 435.49 | 3.37 | 3.37 |
| 391.44 | 3.55 | 3.55 |
| 373.45 | 3.41 | 3.41 |
| 429.51 | 3.66 | 3.66 |
| 447.50 | 3.79 | 3.79 |
| 457.56 | 4.05 | 4.05 |
| 477.54 | 5.06 | 5.06 |
| 512.69 | 5.55 | 5.55 |
| 457.56 | 4.42 | 4.42 |
| 443.54 | 3.67 | 3.67 |
| 461.53 | 3.80 | 3.80 |
| 456.58 | 3.58 | 3.58 |
| 478.58 | 3.12 | 3.12 |
| 420.50 | 3.46 | 3.46 |
| 464.55 | 3.00 | 3.00 |
| 434.53 | 3.46 | 3.46 |
| 478.58 | 2.99 | 2.99 |
| 447.57 | 3.77 | 3.77 |
| 478.58 | 3.75 | 3.75 |
| 434.53 | 4.22 | 4.22 |
| 509.61 | 3.44 | 3.44 |
| 477.59 | 2.91 | 2.91 |
| 358.43 | 3.83 | 3.83 |
| 456.51 | 4.37 | 4.37 |
| 468.55 | 3.69 | 3.69 |