Assay Report Card

Basic Information

ID: ALA3706373

Type: Binding

Description: Homogeneous Time Resolved Fluorescence Assay: The assay below is used to test the modulatory activity of compounds against FLAP. Human and mouse FLAP-encoding DNA was amplified by polymerase chain reaction and cloned into pFastBacl (Invitrogen) with a NH2-terminal 6-His tag for expression in Spodoptera frugiperda (Sf-9) cells. FLAP-containing membranes were prepared as was a FITC-labeled FLAP modulator [5-[({[2-(2-{3-[3-(tert-Butylsulfanyl)-1-(4-chlorobenzyl)-5-(quinolin-2-ylmethoxy)-1H-indol-2-yl]-2,2-dimethylpropanoyl}hydrazino)-2-oxoethyl]sulfanyl}acetyl)amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid]. The FLAP binding assay is performed in HTRF format (homogeneous time resolved fluorescence). FLAP-containing membranes (1 ug/well final for human) are incubated in the presence of the HTRF ligand, [5-[({[2-(2-{3-[3-(tert-butylsulfanyl)-1-(4-chlorobenzyl)-5-(quinolin-2-ylmethoxy)-1H-indol-2-yl]-2,2-dimethylpropanoyl}hydrazino)-2-oxoethyl]sulfanyl}acetyl)amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid].

Organism: Homo sapiens

Activity Charts

Bioactivity

Activity Types for Assay ALA3706373

Total(833)

Compound Summary

Parent Molecular Weight	ALogP	Polar Surface Area
343.38	2.94	2.94
358.40	2.90	2.90
368.39	2.81	2.81
400.48	3.61	3.61
344.37	2.18	2.18
426.52	4.14	4.14
400.48	3.47	3.47
440.42	3.76	3.76
412.49	3.61	3.61
438.53	4.00	4.00
412.49	3.75	3.75
414.51	4.00	4.00
454.45	4.15	4.15
412.49	3.75	3.75
358.40	2.44	2.44
372.43	2.83	2.83
448.52	4.05	4.05
502.49	5.11	5.11
440.42	3.76	3.76
460.53	3.98	3.98
460.53	4.50	4.50
420.47	4.33	4.33
483.57	3.21	3.21
455.52	2.48	2.48
469.54	2.82	2.82
497.64	4.46	4.46
421.46	3.73	3.73
402.45	2.19	2.19
359.39	2.15	2.15
386.45	3.68	3.68
372.43	3.29	3.29
386.45	3.68	3.68
400.48	3.93	3.93
384.44	3.44	3.44
428.53	4.85	4.85
398.46	3.83	3.83
412.37	3.79	3.79
372.43	2.78	2.78
412.49	3.71	3.71
414.46	2.55	2.55
462.53	1.95	1.95
413.48	2.13	2.13
400.48	3.56	3.56
398.46	3.32	3.32
448.47	3.95	3.95
448.47	3.95	3.95
434.44	3.56	3.56
420.42	3.17	3.17
426.52	4.10	4.10
427.51	2.47	2.47
496.49	3.61	3.61
455.52	2.38	2.38
481.55	2.77	2.77
457.53	1.83	1.83
453.54	3.00	3.00
453.54	3.00	3.00
441.53	2.90	2.90
437.50	3.46	3.46
437.50	3.60	3.60
427.51	2.64	2.64
427.46	1.65	1.65
428.49	2.68	2.68
442.52	2.92	2.92
456.54	3.32	3.32
428.49	2.68	2.68
456.54	3.32	3.32
441.53	2.90	2.90
455.56	3.25	3.25
427.51	2.64	2.64
469.54	2.82	2.82
442.52	2.92	2.92
527.62	4.21	4.21
494.55	2.90	2.90
444.53	3.22	3.22
402.45	2.19	2.19
464.52	3.54	3.54
464.52	3.54	3.54
478.55	3.93	3.93
444.53	3.22	3.22
442.52	3.11	3.11
416.48	2.58	2.58
442.52	3.11	3.11
388.42	1.80	1.80
384.44	2.97	2.97
398.46	3.22	3.22
413.48	2.25	2.25
428.49	2.68	2.68
422.46	1.77	1.77
402.45	2.19	2.19
456.54	3.51	3.51
416.48	2.58	2.58
416.48	2.58	2.58
418.45	1.16	1.16
430.46	1.26	1.26
427.46	1.70	1.70
441.49	2.09	2.09
442.52	3.11	3.11
428.49	2.53	2.53
441.53	2.90	2.90
478.55	3.41	3.41
400.44	1.90	1.90
442.52	3.11	3.11
456.54	3.36	3.36
427.51	2.56	2.56
413.48	2.11	2.11
413.48	2.03	2.03
450.50	3.00	3.00
495.54	2.44	2.44
425.49	2.27	2.27
430.51	2.97	2.97
444.53	3.36	3.36
441.53	3.08	3.08
470.57	3.85	3.85
427.51	2.64	2.64
432.48	1.51	1.51
427.46	1.78	1.78
468.55	3.86	3.86
430.51	2.92	2.92
444.49	1.91	1.91
427.51	2.51	2.51
432.48	1.55	1.55
430.51	2.83	2.83
444.53	3.22	3.22
487.56	3.33	3.33
387.44	1.77	1.77
416.48	2.58	2.58
413.48	2.17	2.17
429.48	1.95	1.95
416.43	2.28	2.28
430.47	1.48	1.48
476.53	3.47	3.47
489.45	4.75	4.75
478.55	3.82	3.82
465.51	2.69	2.69
482.50	3.14	3.14
436.47	3.43	3.43
457.53	2.71	2.71
397.44	2.72	2.72
411.46	3.11	3.11
437.50	3.60	3.60
414.46	2.60	2.60
466.54	2.75	2.75
416.48	2.85	2.85
280.31	3.11	3.11
490.56	3.81	3.81
413.48	3.28	3.28
387.44	2.75	2.75
429.48	2.52	2.52
414.46	2.29	2.29
414.46	2.29	2.29
483.57	2.36	2.36
499.57	1.60	1.60
365.36	5.14	5.14
353.47	5.42	5.42
357.41	3.33	3.33
371.44	3.72	3.72
385.46	3.96	3.96
385.46	4.10	4.10
397.48	4.25	4.25
383.45	3.86	3.86
369.42	3.47	3.47
413.52	4.89	4.89
371.44	3.71	3.71
354.41	3.11	3.11
410.52	4.49	4.49
424.50	3.48	3.48
396.45	3.73	3.73
444.54	5.05	5.05
465.58	5.23	5.23
412.37	3.20	3.20
411.38	3.95	3.95
495.46	2.67	2.67
440.42	3.85	3.85
495.50	3.49	3.49
375.43	4.47	4.47
375.43	4.47	4.47
390.45	4.06	4.06
390.45	4.06	4.06
390.45	4.06	4.06
375.43	4.47	4.47
390.45	4.06	4.06
372.43	2.58	2.58
402.45	1.94	1.94
414.46	2.04	2.04
441.49	1.79	1.79
416.48	2.33	2.33
416.48	2.33	2.33
456.54	3.25	3.25
416.48	2.33	2.33
416.48	2.33	2.33
441.53	2.78	2.78
358.40	2.32	2.32
386.45	2.97	2.97
484.56	2.49	2.49
483.57	2.96	2.96
339.44	4.79	4.79
325.41	4.39	4.39
353.47	5.17	5.17
404.47	4.20	4.20
404.47	4.20	4.20
389.46	4.61	4.61
404.47	4.20	4.20
461.54	2.88	2.88
390.45	4.06	4.06
389.46	4.61	4.61
427.62
459.62
449.53	2.74	2.74
427.51	2.51	2.51
416.48	2.58	2.58
437.50	3.60	3.60
397.44	2.72	2.72
430.51	2.83	2.83
444.49	1.91	1.91
427.51	2.51	2.51
369.42	3.47	3.47
432.48	1.55	1.55
441.53	2.90	2.90
441.53	2.90	2.90
443.50	1.49	1.49
442.52	3.11	3.11
442.52	3.11	3.11
430.51	2.92	2.92
442.52	3.11	3.11
427.46	1.70	1.70
462.53	1.95	1.95
400.44	1.90	1.90
441.49	2.09	2.09
441.49	2.09	2.09
440.42	3.76	3.76
444.53	3.22	3.22
444.53	3.22	3.22
430.51	2.83	2.83
416.48	2.58	2.58
430.51	2.83	2.83
388.42	1.80	1.80
428.49	2.68	2.68
428.49	2.68	2.68
402.45	2.19	2.19
416.48	2.58	2.58
453.54	3.00	3.00
457.53	1.83	1.83
398.46	3.22	3.22
384.44	2.97	2.97
420.47	4.33	4.33
402.45	2.19	2.19
414.51	3.92	3.92
358.40	2.44	2.44
413.48	2.13	2.13
502.49	5.11	5.11
385.46	4.10	4.10
400.48	3.47	3.47
372.43	2.83	2.83
454.45	4.15	4.15
440.42	3.76	3.76
398.46	3.32	3.32
372.43	2.78	2.78
400.48	3.56	3.56
412.49	3.71	3.71
344.37	2.18	2.18
414.46	2.55	2.55
343.38	2.94	2.94
400.48	3.61	3.61
427.46	1.65	1.65
472.54	3.54	3.54
416.43	2.28	2.28
413.48	3.28	3.28
429.48	2.52	2.52
387.44	2.75	2.75
426.47	2.55	2.55
474.54	1.95	1.95
409.45	2.68	2.68
468.43	2.83	2.83
461.54	2.88	2.88
487.52	3.74	3.74
503.56	4.04	4.04
517.65	4.44	4.44
459.51	4.25	4.25
451.48	3.77	3.77
412.37	3.20	3.20
411.38	3.95	3.95
495.46	2.67	2.67
426.39	3.46	3.46
456.42	2.82	2.82
440.42	3.85	3.85
495.50	3.49	3.49
390.45	4.06	4.06
390.45	4.06	4.06
390.45	4.06	4.06
375.43	4.47	4.47
375.43	4.47	4.47
390.45	4.06	4.06
390.45	4.06	4.06
390.45	4.06	4.06
422.45	2.74	2.74
459.62
422.45	2.74	2.74
427.62
342.40	3.54	3.54
399.49	4.21	4.21
399.49	4.17	4.17
397.48	3.92	3.92
411.50	4.31	4.31
425.53	4.75	4.75
401.46	2.80	2.80
399.49	4.07	4.07
413.52	4.60	4.60
453.46	4.76	4.76
501.51	5.72	5.72
411.50	4.36	4.36
461.54	2.56	2.56
439.43	4.37	4.37
423.51	4.31	4.31
528.61	3.40	3.40
428.49	1.75	1.75
455.56	3.92	3.92
427.50	3.28	3.28
441.53	3.53	3.53
425.49	3.70	3.70
425.49	3.70	3.70
450.50	3.20	3.20
392.46	4.97	4.97
411.46	3.31	3.31
423.51	5.11	5.11
367.40	3.68	3.68
382.42	3.80	3.80
396.45	3.68	3.68
424.50	4.51	4.51
422.49	4.22	4.22
424.50	4.46	4.46
436.51	4.61	4.61
438.48	3.45	3.45
367.41	3.83	3.83
426.47	3.09	3.09
382.42	3.34	3.34
426.47	3.09	3.09
451.53	3.54	3.54
353.40	5.29	5.29
349.43	5.54	5.54
384.43	3.76	3.76
453.54	4.53	4.53
385.42	3.01	3.01
443.50	3.02	3.02
468.55	3.47	3.47
441.53	4.39	4.39
503.58	2.78	2.78
441.53	4.44	4.44
432.45	6.35	6.35
372.43	3.11	3.11
340.43	4.43	4.43
388.32	4.77	4.77
362.41	4.86	4.86
348.38	4.61	4.61
360.39	4.61	4.61
324.36	3.71	3.71
350.40	4.25	4.25
364.42	4.64	4.64
296.31	2.94	2.94
336.37	3.86	3.86
465.53	4.56	4.56
365.41	2.92	2.92
324.36	3.71	3.71
281.29	2.51	2.51
427.46	2.06	2.06
394.43	3.34	3.34
427.46	2.06	2.06
413.43	1.67	1.67
452.47	1.52	1.52
427.46	2.06	2.06
403.42	2.88	2.88
400.48	3.61	3.61
424.50	4.51	4.51
435.52	5.18	5.18
435.52	5.18	5.18
422.53	5.72	5.72
423.51	5.11	5.11
381.43	3.94	3.94
440.57	5.85	5.85
423.51	5.11	5.11
381.43	3.94	3.94
399.49	4.21	4.21
415.49	3.19	3.19
441.49	3.20	3.20
417.48	4.35	4.35
425.53	4.60	4.60
424.50	4.51	4.51
430.51	3.62	3.62
425.49	3.48	3.48
425.49	3.48	3.48
424.50	4.09	4.09
424.50	4.09	4.09
406.51	3.67	3.67
408.48	2.25	2.25
468.56	2.01	2.01
434.92	4.26	4.26
479.38	4.37	4.37
393.44	3.94	3.94
424.50	4.28	4.28
414.51	3.92	3.92
358.40	2.49	2.49
425.49	3.90	3.90
478.39	4.98	4.98
372.43	2.75	2.75
424.50	4.09	4.09
433.94	4.87	4.87
467.49	5.23	5.23
468.48	4.63	4.63
414.51	3.92	3.92
453.54	4.61	4.61
479.58	4.81	4.81
411.46	3.44	3.44
437.54	5.30	5.30
322.34	3.49	3.49
323.33	3.02	3.02
361.37	3.07	3.07
418.47	3.75	3.75
372.43	3.21	3.21
362.36	2.32	2.32
376.39	2.58	2.58
418.47	3.75	3.75
367.28	4.57	4.57
283.28	3.67	3.67
299.74	4.19	4.19
279.32	3.84	3.84
351.28	4.69	4.69
333.29	4.55	4.55
295.32	3.54	3.54
311.38	4.25	4.25
349.29	4.43	4.43
281.29	3.24	3.24
405.45	4.36	4.36
301.27	3.81	3.81
419.48	4.51	4.51
293.35	4.09	4.09
339.33	3.00	3.00
307.37	4.66	4.66
307.33	3.73	3.73
363.31	4.47	4.47
309.30	3.23	3.23
308.32	2.63	2.63
323.37	4.32	4.32
336.37	3.72	3.72
385.39	3.97	3.97
372.43	2.93	2.93
396.45	4.19	4.19
396.45	3.83	3.83
410.47	4.22	4.22
386.45	3.32	3.32
424.50	4.83	4.83
386.45	3.32	3.32
414.51	3.95	3.95
401.44	4.51	4.51
378.41	3.00	3.00
402.43	3.90	3.90
364.42	4.06	4.06
382.41	4.20	4.20
364.42	4.01	4.01
404.37	4.21	4.21
404.37	4.21	4.21
377.42	2.58	2.58
419.46	2.84	2.84
390.46	4.59	4.59
426.47	2.40	2.40
322.34	2.89	2.89
404.37	4.21	4.21
366.40	2.64	2.64
366.40	2.64	2.64
392.43	3.13	3.13
391.45	3.10	3.10
426.47	2.40	2.40
366.40	2.64	2.64
404.37	4.21	4.21
391.41	2.10	2.10
391.41	2.10	2.10
446.40	4.02	4.02
446.40	4.02	4.02
397.41	5.20	5.20
397.41	5.20	5.20
382.40	5.62	5.62
359.36	4.11	4.11
359.36	4.11	4.11
374.38	3.70	3.70
374.38	3.70	3.70
397.41	4.38	4.38
397.41	4.38	4.38
383.39	4.59	4.59
374.38	3.70	3.70
374.38	3.70	3.70
374.38	3.70	3.70
374.38	3.70	3.70
383.39	4.59	4.59
383.39	4.59	4.59
414.44	4.32	4.32
414.44	4.32	4.32
373.39	4.42	4.42
373.39	4.42	4.42
382.40	5.62	5.62
383.39	5.01	5.01
373.39	4.42	4.42
373.39	4.42	4.42
427.36	5.13	5.13
427.36	5.13	5.13
389.39	4.12	4.12
389.39	4.12	4.12
427.36	5.13	5.13
363.31	4.56	4.56
349.29	4.26	4.26
442.38	4.72	4.72
442.38	4.72	4.72
479.55
349.29	4.26	4.26
442.38	4.72	4.72
442.38	4.72	4.72
442.38	4.72	4.72
401.29	5.57	5.57
358.30	4.42	4.42
410.36	4.39	4.39
425.37	3.97	3.97
342.36	3.37	3.37
357.38	2.95	2.95
357.38	2.95	2.95
396.52	3.78	3.78
412.52	3.48	3.48
382.49	3.47	3.47
354.44	2.64	2.64
396.47	2.41	2.41
400.48	3.61	3.61
372.43	2.78	2.78
389.45	3.81	3.81
405.52	4.28	4.28
405.52	4.23	4.23
403.50	3.98	3.98
441.50	5.02	5.02
438.53	4.47	4.47
466.54	3.98	3.98
448.52	4.76	4.76
448.52	4.76	4.76
491.51	5.90	5.90
495.58	5.06	5.06
453.54	4.89	4.89
325.39	2.80	2.80
339.42	3.10	3.10
368.46	2.95	2.95
342.38	1.75	1.75
340.41	2.76	2.76
326.38	2.04	2.04
438.52	4.73	4.73
467.52	3.77	3.77
438.53	4.70	4.70
444.49	3.31	3.31
386.41	1.79	1.79
442.52	3.17	3.17
456.50	2.16	2.16
452.47	5.38	5.38
377.30	4.25	4.25
361.30	4.36	4.36
432.42	4.38	4.38
445.46	4.47	4.47
406.38	3.63	3.63
387.42	4.73	4.73
387.42	4.73	4.73
426.37	5.74	5.74
426.37	5.74	5.74
427.36	5.13	5.13
427.36	5.13	5.13
383.39	4.59	4.59
383.39	4.59	4.59
373.39	4.30	4.30
373.39	4.30	4.30
388.41	4.00	4.00
388.41	4.00	4.00
426.37	5.74	5.74
383.39	4.59	4.59
383.39	4.59	4.59
325.34	2.90	2.90
325.34	2.90	2.90
364.42	3.66	3.66
364.42	3.66	3.66
364.42	3.66	3.66
364.42	3.66	3.66
399.43	4.99	4.99
399.43	4.99	4.99
373.39	4.30	4.30
373.39	4.30	4.30
324.36	2.87	2.87
324.36	2.87	2.87
320.33	3.43	3.43
320.33	3.43	3.43
339.33	3.00	3.00
326.33	2.30	2.30
326.33	2.30	2.30
379.44	3.58	3.58
379.44	3.58	3.58
366.40	3.48	3.48
366.40	3.48	3.48
340.36	2.69	2.69
340.36	2.69	2.69
357.41	3.51	3.51
413.47	3.16	3.16
357.41	3.05	3.05
343.38	2.78	2.78
425.49	3.30	3.30
429.48	1.14	1.14
415.49	3.19	3.19
491.59	4.88	4.88
433.48	3.33	3.33
445.49	3.42	3.42
509.58	5.02	5.02
486.54	3.57	3.57
473.55	4.06	4.06
459.52	3.67	3.67
403.44	1.59	1.59
441.41	3.24	3.24
401.47	3.00	3.00
415.42	3.64	3.64
361.41	2.26	2.26
278.31	2.80	2.80
431.47	3.21	3.21
417.44	2.82	2.82
409.52	3.35	3.35
431.47	3.21	3.21
355.42	2.09	2.09
421.53	3.25	3.25
423.42	3.02	3.02
423.42	3.02	3.02
410.46	0.91	0.91
385.45	1.45	1.45
385.45	1.45	1.45
439.42	2.08	2.08
352.42	2.72	2.72
423.42	3.10	3.10
383.48	2.86	2.86
385.45	1.45	1.45
399.39	3.57	3.57
362.18	4.43	4.43
385.21	5.94	5.94
363.31	4.56	4.56
327.38	3.27	3.27
290.30	3.40	3.40
349.37	3.06	3.06
425.49	3.48	3.48
487.54	1.82	1.82
441.49	1.90	1.90
413.43	1.67	1.67
427.46	2.06	2.06
427.46	2.06	2.06
455.52	2.42	2.42
427.46	2.06	2.06
427.46	2.06	2.06
481.43	2.69	2.69
465.43	3.72	3.72
455.52	2.70	2.70
520.47	2.54	2.54
476.53	3.74	3.74
427.50	3.92	3.92
407.43	3.15	3.15
407.43	3.15	3.15
407.43	3.15	3.15
422.45	2.74	2.74
422.45	2.74	2.74
422.45	2.74	2.74
422.45	2.74	2.74
422.45	2.74	2.74
422.45	2.74	2.74
383.45	3.72	3.72
394.43	3.34	3.34
422.49	4.14	4.14
440.50	3.10	3.10
396.45	3.61	3.61
414.46	2.57	2.57
400.48	3.04	3.04
396.45	3.61	3.61
414.46	2.57	2.57
400.48	3.04	3.04
422.49	4.14	4.14
440.50	3.10	3.10
426.52	3.58	3.58
390.45	4.06	4.06
390.45	4.06	4.06
375.43	4.47	4.47
390.45	4.06	4.06
399.39	3.57	3.57
452.37	5.00	5.00
384.37	3.99	3.99
478.57	3.40	3.40
371.44	3.47	3.47
357.41	3.08	3.08
385.46	3.86	3.86
421.46	3.30	3.30
446.40	3.94	3.94
364.38	2.35	2.35
392.43	3.44	3.44
494.47	3.42	3.42
422.46	2.75	2.75
422.46	2.75	2.75
352.37	2.25	2.25
352.37	2.25	2.25
392.43	3.13	3.13
406.46	3.57	3.57
392.43	3.13	3.13
406.46	3.57	3.57
446.40	3.94	3.94
392.43	3.13	3.13
392.43	3.13	3.13
378.41	2.74	2.74
378.41	2.74	2.74
378.41	2.74	2.74
378.41	2.74	2.74
406.46	3.78	3.78
392.43	3.39	3.39
392.43	3.39	3.39
406.46	3.78	3.78
402.43	3.90	3.90
379.40	2.40	2.40
379.40	2.40	2.40
432.42	5.08	5.08
434.39	3.66	3.66
434.39	3.66	3.66
472.36	5.23	5.23
472.36	5.23	5.23
472.36	5.23	5.23
472.36	5.23	5.23
364.38	2.35	2.35
405.48	3.36	3.36
494.47	3.42	3.42
432.42	5.08	5.08
460.43	4.15	4.15
460.43	4.15	4.15
392.43	3.44	3.44
408.43	2.37	2.37
408.43	2.37	2.37
365.41	3.91	3.91
365.41	3.91	3.91
388.45	5.42	5.42
399.45	2.06	2.06
399.45	2.06	2.06
384.44	3.25	3.25
384.44	3.25	3.25
412.49	4.03	4.03
412.49	4.03	4.03
398.46	3.64	3.64
398.46	3.64	3.64
386.45	3.50	3.50
386.45	3.50	3.50
386.45	3.50	3.50
386.45	3.50	3.50
455.52	2.71	2.71
455.52	2.71	2.71
402.45	2.74	2.74
402.45	2.74	2.74
462.53	2.28	2.28
462.53	2.28	2.28
443.50	2.03	2.03
443.50	2.03	2.03
428.49	3.13	3.13
428.49	3.13	3.13
372.43	3.11	3.11
395.46	4.61	4.61
413.48	2.45	2.45
370.41	2.86	2.86
370.41	2.86	2.86
414.46	2.88	2.88
414.46	2.88	2.88
413.48	2.45	2.45
283.28	3.67	3.67
295.32	3.54	3.54
373.41	2.94	2.94
430.51	3.62	3.62
388.42	2.91	2.91
343.37	3.99	3.99
343.37	3.99	3.99
358.38	3.57	3.57
358.38	3.57	3.57
358.38	3.57	3.57
358.38	3.57	3.57
358.38	3.57	3.57
426.38	4.59	4.59
373.39	4.00	4.00
345.38	3.93	3.93
343.37	3.99	3.99
368.38	3.86	3.86
428.47	3.83	3.83
403.46	4.17	4.17
414.41	4.93	4.93
426.38	4.59	4.59
359.37	2.97	2.97
341.38	2.83	2.83
384.44	3.07	3.07
398.46	3.46	3.46
384.44	3.07	3.07
335.39	3.53	3.53
349.37	3.05	3.05
448.52	4.57	4.57
428.49	2.68	2.68
427.51	2.64	2.64
402.45	2.19	2.19
418.45	1.16	1.16
430.51	2.83	2.83
428.49	2.53	2.53
423.45	2.15	2.15
444.53	3.22	3.22
526.64	1.95	1.95
425.49	2.11	2.11
464.52	3.54	3.54
340.36	2.69	2.69
364.42	3.66	3.66
411.46	3.11	3.11
364.42	3.66	3.66
340.36	2.69	2.69
427.51	2.51	2.51
441.53
402.45	2.19	2.19
427.51	2.51	2.51
441.53	2.90	2.90
476.53	3.47	3.47
502.49	5.11	5.11
443.50	1.49	1.49
427.51	2.56	2.56