Basic Information
ID: ALA3707582
Type: Binding
Description: Kinase Inhibition Assay: The buffer for PIM-2 assay was composed of HEPES 50 mM, at pH 7.5, with 1 mM MgCl2, 1 mM DTT, 3 microM Na3VO4, and 0.2 mg/mL BSAFull-length human PIM-2 was expressed and purified as described in Fedorov O, et al., PNAS 2007 104, 51, 20523-28.Assay Conditions (Final Concentrations)Enzyme concentration=1.5 nMAktide substrate (Chemical Abstract Service Registry Number 324029-01-8)=5 microMATP=4 microM33P-γ-ATP=1 nM.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3707582
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 243.27 | 0.26 | 0.26 |
| 303.32 | 2.46 | 2.46 |
| 319.37 | 1.93 | 1.93 |
| 317.35 | 2.47 | 2.47 |
| 647.87 | 7.08 | 7.08 |
| 461.57 | 2.49 | 2.49 |
| 302.40 | 2.45 | 2.45 |
| 304.38 | 0.86 | 0.86 |
| 316.39 | 1.71 | 1.71 |
| 314.41 | 3.29 | 3.29 |
| 343.46 | 1.79 | 1.79 |
| 357.48 | 2.31 | 2.31 |
| 355.47 | 2.85 | 2.85 |
| 341.44 | 2.33 | 2.33 |
| 258.31 | 1.93 | 1.93 |
| 343.46 | 2.39 | 2.39 |
| 593.78 | 5.85 | 5.85 |