Basic Information
ID: ALA3707599
Type: Binding
Description: Enzyme Inhibition Assay: Enzyme activity is measured by observing the increase in fluorescence intensity caused by cleavage of a peptide substrate containing a fluorophore whose emission is quenched in the intact peptide. Assay buffer: 100 mM potassium phosphate pH 6.5, EDTA-Na 5 mM, Triton X-100 0.001%, DTT 5 mM. Enzymes (all at 1 nM): human and mouse Cathepsin S, Cat K, Cat B, Cat L. Substrate (20 μM): Z-Val-Val-Arg-AMC, except for Cat K which uses Z-Leu-Arg-AMC (both from Bachem).Z=Benzyloxycarbonyl.AMC=7-Amino-4-Methyl-Coumarin. DTT=dithiothreitol. Final volume: 100 μL. Excitation 360 nm, Emission 465 nm.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3707599
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 535.66 | 2.20 | 2.20 |
| 556.08 | 2.54 | 2.54 |
| 596.58 | 2.78 | 2.78 |
| 627.66 | 3.24 | 3.24 |
| 630.60 | 4.95 | 4.95 |
| 688.57 | 3.86 | 3.86 |
| 611.56 | 2.53 | 2.53 |
| 620.55 | 3.21 | 3.21 |
| 634.58 | 3.60 | 3.60 |
| 737.56 | 3.69 | 3.69 |
| 752.13 | 4.52 | 4.52 |
| 654.57 | 5.39 | 5.39 |
| 638.54 | 3.35 | 3.35 |
| 683.00 | 3.46 | 3.46 |
| 665.01 | 3.32 | 3.32 |
| 679.03 | 3.71 | 3.71 |
| 712.01 | 3.16 | 3.16 |
| 726.03 | 3.55 | 3.55 |
| 673.09 | 3.11 | 3.11 |
| 687.11 | 3.50 | 3.50 |
| 654.11 | 3.58 | 3.58 |
| 668.13 | 3.97 | 3.97 |
| 683.98 | 2.85 | 2.85 |
| 653.56 | 3.14 | 3.14 |
| 639.53 | 2.75 | 2.75 |
| 620.55 | 3.21 | 3.21 |
| 618.54 | 2.96 | 2.96 |
| 634.58 | 3.46 | 3.46 |
| 648.61 | 3.85 | 3.85 |
| 688.67 | 4.77 | 4.77 |
| 636.60 | 3.85 | 3.85 |
| 650.63 | 4.24 | 4.24 |
| 670.62 | 4.25 | 4.25 |
| 606.53 | 2.82 | 2.82 |
| 684.64 | 4.64 | 4.64 |
| 650.63 | 4.24 | 4.24 |
| 664.65 | 4.63 | 4.63 |
| 655.00 | 3.86 | 3.86 |
| 638.54 | 3.35 | 3.35 |
| 676.54 | 4.00 | 4.00 |
| 636.60 | 3.85 | 3.85 |
| 669.03 | 4.25 | 4.25 |
| 608.54 | 3.07 | 3.07 |
| 622.57 | 3.46 | 3.46 |
| 732.61 | 4.54 | 4.54 |
| 746.63 | 4.93 | 4.93 |
| 648.61 | 3.94 | 3.94 |
| 652.57 | 3.74 | 3.74 |
| 684.64 | 4.29 | 4.29 |
| 734.70 | 5.44 | 5.44 |
| 734.70 | 5.44 | 5.44 |
| 720.67 | 5.40 | 5.40 |
| 664.61 | 3.23 | 3.23 |
| 638.57 | 2.69 | 2.69 |
| 636.60 | 3.70 | 3.70 |
| 705.66 | 3.05 | 3.05 |
| 719.69 | 3.44 | 3.44 |
| 653.56 | 2.99 | 2.99 |
| 667.59 | 3.38 | 3.38 |
| 655.58 | 3.38 | 3.38 |
| 669.60 | 3.77 | 3.77 |
| 703.62 | 4.17 | 4.17 |