ID: ALA3707644
Type: Binding
Description: Enzyme Assay: The 11-β-HSD-1 enzyme assay was carried out in 96 well microtiter plates in a total volume of 100 μl containing 30 mM Hepes buffer, pH 7.4 with 1 mM EDTA, substrate mixture cortisone/NADPH (200 nM/200 μM), G-6-P (1 mM) and inhibitors in serial dilutions. Reactions were initiated by addition of 10 μl 11-β-HSD-1 (3 μg) from E. coli, either as microsome fractions from rat and mice liver (2.5 μg). Following mixing, the plates were shaken for 150 minutes at 37° C. The reactions were terminated with 10 μl Acid-18beta glycyrrhetinic stop solution. Determinations of cortisol levels in 11-β-HSD-1 preparations were monitored by HTRF (HTRF cortisol assay from Cis bio international).
Organism: Rattus norvegicus
Tissue: Liver
Bioactivity
Activity Types for Assay ALA3707644
IC50
Parent Molecular Weight | ALogP | Polar Surface Area |
---|---|---|
361.42 | 3.68 | 3.68 |
361.42 | 3.53 | 3.53 |
377.88 | 4.19 | 4.19 |