Basic Information
ID: ALA3707813
Type: Binding
Description: Luciferase-Based Assay: Recombinant human c-Src or Yes (28 ng/well, Panvera/Invitrogen, Madison Wis.), ATP (3 μM), a tyrosine kinase substrate (PTK2, 250 μM, Promega Corp., Madison Wis.), and test agents (at concentrations ranging from about 1 nM/l to about 100 μM/l), in the presence of Src kinase reaction buffer (Upstate USA, Lake Placid N.Y.). After reacting for about 90 minutes at room temperature, residual ATP was determined using a luciferase-based assay (KinaseGlo, Promega Corp.) as a measure of kinase activity.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3707813
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 340.43 | 5.36 | 5.36 |
| 494.43 | 6.53 | 6.53 |
| 516.67 | 4.73 | 4.73 |
| 516.67 | 4.73 | 4.73 |
| 502.64 | 4.43 | 4.43 |
| 520.63 | 4.57 | 4.57 |
| 482.63 | 4.99 | 4.99 |
| 496.62 | 5.49 | 5.49 |
| 532.67 | 4.90 | 4.90 |
| 475.98 | 5.58 | 5.58 |
| 474.95 | 4.15 | 4.15 |
| 539.06 | 4.48 | 4.48 |
| 553.09 | 4.82 | 4.82 |
| 538.07 | 5.36 | 5.36 |
| 510.02 | 4.63 | 4.63 |
| 573.51 | 5.13 | 5.13 |
| 469.59 | 5.54 | 5.54 |
| 496.66 | 5.38 | 5.38 |
| 496.66 | 5.38 | 5.38 |
| 470.58 | 4.78 | 4.78 |
| 487.63 | 4.64 | 4.64 |
| 503.01 | 4.79 | 4.79 |
| 487.01 | 5.09 | 5.09 |
| 488.98 | 4.49 | 4.49 |
| 457.55 | 5.67 | 5.67 |
| 441.54 | 4.92 | 4.92 |
| 363.81 | 4.50 | 4.50 |
| 559.70 | 4.38 | 4.38 |
| 492.97 | 4.52 | 4.52 |
| 518.64 | 4.13 | 4.13 |
| 476.97 | 4.39 | 4.39 |
| 405.89 | 5.17 | 5.17 |
| 539.06 | 4.48 | 4.48 |
| 448.91 | 4.33 | 4.33 |
| 475.98 | 5.58 | 5.58 |
| 452.60 | 7.32 | 7.32 |
| 488.62 | 3.99 | 3.99 |