Assay Report Card

Basic Information

ID: ALA3885767

Type: Binding

Description: Biochemical assay using fluorogenic peptide substrate.Step 1: Compound transfer to plate (50 nl).Step 2: Dilute FXa in Assay Buffer.Step 3: Dilute substrate in Assay Buffer.Step 4: Add equal volumes to Assay plate (total assay volume 10 uL).Step 5: Centrifuge plate, 1 min, 1000 rpm.Step 6: Detect fluorescence signal: ex: 320 nm; Em. 405 nm (EnVision), every 10 min. 60 min pre-incubation of enzyme with compound and 45 min reaction time after substrate addition.

Organism: Homo sapiens

Activity Charts

Bioactivity

Activity Types for Assay ALA3885767

IC50

Total(55)

Compound Summary

Parent Molecular Weight	ALogP	Polar Surface Area
446.51	2.42	2.42
462.98	2.87	2.87
453.55	2.51	2.51
424.55	4.83	4.83
455.57	2.17	2.17
497.06	5.97	5.97
421.91	2.99	2.99
423.90	3.90	3.90
379.87	3.22	3.22
438.91	2.71	2.71
419.89	3.28	3.28
453.88	2.66	2.66
503.89	3.54	3.54
435.89	2.52	2.52
480.34	2.63	2.63
415.47	2.17	2.17
464.27	2.16	2.16
450.90	2.10	2.10
435.93	3.30	3.30
455.96	3.85	3.85
439.90	3.13	3.13
429.86	2.46	2.46
429.86	2.46	2.46
429.86	2.46	2.46
436.88	1.91	1.91
430.85	1.85	1.85
430.85	1.85	1.85
449.92	2.86	2.86
471.94	2.07	2.07
472.89	3.17	3.17
492.94	3.08	3.08
474.95	3.13	3.13
427.51	2.25	2.25
529.04	3.35	3.35
526.02	4.51	4.51
497.44	4.65	4.65
475.88	3.54	3.54
473.87	3.26	3.26
513.96	3.41	3.41
563.99	2.48	2.48
465.54	2.39	2.39
490.94	2.11	2.11
407.88	2.52	2.52
499.92	3.52	3.52
504.97	3.60	3.60
438.92	2.12	2.12
532.00	2.57	2.57
517.95	4.62	4.62
444.51	3.22	3.22
489.96	3.85	3.85
486.96	3.35	3.35
552.06	4.27	4.27
618.11	2.65	2.65
504.97	2.76	2.76
458.90	2.31	2.31