Basic Information
ID: ALA3885768
Type: Binding
Description: Biochemical assay using fluorogenic peptide substrate.Step 1: Compound transfer to plate (50 nl).Step 2: Dilute FXa in Assay Buffer.Step 3: Dilute substrate in Assay Buffer.Step 4: Add equal volumes to Assay plate (total assay volume 10 uL).Step 5: Centrifuge plate, 1 min, 1000 rpm.Step 6: Detect fluorescence signal: ex: 320 nm; Em. 405 nm (EnVision), every 10 min. 60 min pre-incubation of enzyme with compound and 30 min reaction time after substrate addition.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3885768
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 446.51 | 2.42 | 2.42 |
| 462.98 | 2.87 | 2.87 |
| 453.55 | 2.51 | 2.51 |
| 424.55 | 4.83 | 4.83 |
| 455.57 | 2.17 | 2.17 |
| 497.06 | 5.97 | 5.97 |
| 421.91 | 2.99 | 2.99 |
| 423.90 | 3.90 | 3.90 |
| 379.87 | 3.22 | 3.22 |
| 438.91 | 2.71 | 2.71 |
| 419.89 | 3.28 | 3.28 |
| 453.88 | 2.66 | 2.66 |
| 503.89 | 3.54 | 3.54 |
| 435.89 | 2.52 | 2.52 |
| 480.34 | 2.63 | 2.63 |
| 415.47 | 2.17 | 2.17 |
| 464.27 | 2.16 | 2.16 |
| 450.90 | 2.10 | 2.10 |
| 435.93 | 3.30 | 3.30 |
| 455.96 | 3.85 | 3.85 |
| 439.90 | 3.13 | 3.13 |
| 429.86 | 2.46 | 2.46 |
| 429.86 | 2.46 | 2.46 |
| 429.86 | 2.46 | 2.46 |
| 436.88 | 1.91 | 1.91 |
| 430.85 | 1.85 | 1.85 |
| 430.85 | 1.85 | 1.85 |
| 449.92 | 2.86 | 2.86 |
| 471.94 | 2.07 | 2.07 |
| 472.89 | 3.17 | 3.17 |
| 492.94 | 3.08 | 3.08 |
| 474.95 | 3.13 | 3.13 |
| 427.51 | 2.25 | 2.25 |
| 529.04 | 3.35 | 3.35 |
| 526.02 | 4.51 | 4.51 |
| 497.44 | 4.65 | 4.65 |
| 475.88 | 3.54 | 3.54 |
| 473.87 | 3.26 | 3.26 |
| 513.96 | 3.41 | 3.41 |
| 563.99 | 2.48 | 2.48 |
| 465.54 | 2.39 | 2.39 |
| 490.94 | 2.11 | 2.11 |
| 407.88 | 2.52 | 2.52 |
| 499.92 | 3.52 | 3.52 |
| 504.97 | 3.60 | 3.60 |
| 438.92 | 2.12 | 2.12 |
| 532.00 | 2.57 | 2.57 |
| 517.95 | 4.62 | 4.62 |
| 444.51 | 3.22 | 3.22 |
| 489.96 | 3.85 | 3.85 |
| 486.96 | 3.35 | 3.35 |
| 552.06 | 4.27 | 4.27 |
| 618.11 | 2.65 | 2.65 |
| 504.97 | 2.76 | 2.76 |
| 458.90 | 2.31 | 2.31 |