Basic Information
ID: ALA3885779
Type: Binding
Description: Protein was prepared by dialysis for 24 hours against 1 liter of 30 mM Hepes pH 7.5, 150mM NaCl and 0.5mM TCEP. The final protein concentration was determined by Bradford Assay. The final protein concentration in the sample cell was 50 uM. Ligand stock solutions of 10 mM in DMSO were diluted to 5uM concentrations with ITC buffer and adjusted to 2% (v/v) DMSO. Both the titrate and titrant solutions were degassed prior to loading the calorimeter cell and injection syringe.The integrated heat data were fit with a one-site binding model using the Origin-7 software provided with the MicroCal VP-ITC. Protein concentration was corrected by titration of a reference compound and normalized for the concentration of each ligand. All ligands bound to N-HSP90 have a stoichiometry of approximately unity.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3885779
deltaH
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 283.29 | 1.95 | 1.95 |
| 287.25 | 1.78 | 1.78 |
| 366.15 | 2.54 | 2.54 |
| 430.46 | 3.22 | 3.22 |
| 403.44 | 4.12 | 4.12 |
| 436.49 | 3.28 | 3.28 |
| 386.38 | 2.26 | 2.26 |
| 303.71 | 2.29 | 2.29 |
| 297.31 | 2.20 | 2.20 |
| 407.51 | 4.91 | 4.91 |
| 424.46 | 2.20 | 2.20 |
| 422.44 | 1.81 | 1.81 |
| 400.41 | 2.65 | 2.65 |
| 382.42 | 2.51 | 2.51 |
| 352.39 | 3.88 | 3.88 |
| 382.42 | 2.43 | 2.43 |
| 396.16 | 3.69 | 3.69 |
| 438.89 | 2.32 | 2.32 |
| 312.34 | 4.16 | 4.16 |
| 422.44 | 1.81 | 1.81 |