Basic Information
ID: ALA3887040
Type: Binding
Description: PARP Enzyme Assay: The PARP enzyme assay is set up on ice in a volume of 100 microliters consisting of 100 mM Tris-HCl (pH 8.0), 1 mM MgCl2, 28 mM KCl, 28 mM NaCl, 0.1 mg/ml of DNase I activated herring sperm DNA (Sigma, Mo.), 3.0 micromolar [3H]nicotinamide adenine dinucleotide (470 mci/mmole), 7 micrograms/ml PARP enzyme, and various concentrations of the compounds to be tested. The reaction is initiated by incubating the mixture at 25° C. After 15 minutes of incubation, the reaction is terminated by adding 500 microliters of ice cold 20% (w/v) trichloroacetic acid. The precipitate formed is transferred onto a glass fiber filter (Packard Unifilter-GF/B) and washed three times with ethanol. After the filter is dried, the radioactivity is determined by scintillation counting.
Activity Charts
Bioactivity
Activity Types for Assay ALA3887040
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 349.39 | 2.65 | 2.65 |
| 347.37 | 2.86 | 2.86 |
| 376.46 | 3.22 | 3.22 |
| 392.46 | 2.46 | 2.46 |
| 412.45 | 2.81 | 2.81 |
| 348.36 | 1.63 | 1.63 |
| 392.50 | 3.86 | 3.86 |
| 378.48 | 3.47 | 3.47 |
| 337.38 | 2.51 | 2.51 |
| 349.43 | 4.31 | 4.31 |
| 335.36 | 2.26 | 2.26 |
| 377.44 | 3.29 | 3.29 |
| 391.43 | 3.03 | 3.03 |
| 349.39 | 2.65 | 2.65 |
| 321.34 | 1.87 | 1.87 |
| 378.43 | 2.12 | 2.12 |
| 363.42 | 3.09 | 3.09 |